Citation: WAN Chuan-Ling, XUE Rong, ZHAN You-Yang, LI Xiao-Jing, PEI Feng-Kui. Urinary Metabonomic Study on N-Acetylcysteine-Protected Rats with Chronic Renal Failure from Gd-Diethylenetriamine Pentaacetic Acid Administration[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(9): 1271-1277. doi: 10.11895/j.issn.0253-3820.170307 shu

Urinary Metabonomic Study on N-Acetylcysteine-Protected Rats with Chronic Renal Failure from Gd-Diethylenetriamine Pentaacetic Acid Administration

1.
Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun 130022, China;2 University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 17 April 2017
Revised Date: 20 June 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21305134).

N-Acetylcysteine (NAC) protects rats administrated with gadolinium-based contrast agents from renal injury, however, the underlying mechanisms remain unclear. A 1H NMR-based metabolomics approach coupled with OPLS-DA (orthogonal projection to latent structure with discriminant analysis) was used to analyze the effect of NAC on urinary metabolic changes for Chronic Renal Failure Rats administrated with Gd-DTPA (Gd-Diethylenetriamine pentaacetic acid). Combined with univariate analysis of integral area, the significantly changed metabolites were selected to screen out the potential metabolic disturbances that induced by Gd-DTPA and NAC. These researches may attribute to study of the protective effect of NAC from renal failure induced by gadolinium-based contrast agents. The disturbance of energy metabolism, urea cycle and kynurenine metabolism were observed from the CRF group. Gd-DTPA caused the reduction of urinary choline, TMAO, o-HPA, p-HPA, hippurate, glycine, nicotinate and taurine accompanied with the elevation of allantoin. Metabonomic recovery in the NAC group was observed, which implied that NAC protects rats with chronic renal failure from Gd-DTPA induced disturbances of gut microbiota metabolism, liver mitochondria metabolism and kynurenine metabolism. The replenishment of glutathione in cells and the recovery of urea cycle that caused by NAC may protect rats from oxidative damage and renal injury.
- Metabonomics,
- Nuclear magnetic resonance,
- Chronic renal failure,
- Gd-diethylenetriamine pentaacetic acid,
- N-Acetylcysteine
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沈阳化工大学材料科学与工程学院沈阳 110142