基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用

引用本文: 丁乔棋, 李丽, 范文韬, 吕亚楠, 胡建华, 闫丽萍, 宋素泉. 基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用[J]. 分析化学, 2017, 45(11): 1686-1693. doi: 10.11895/j.issn.0253-3820.170300 shu

Citation: DING Qiao-Qi, LI Li, FAN Wen-Tao, LYU Ya-Nan, HU Jian-Hua, YAN Li-Ping, SONG Su-Quan. Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol[J]. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1686-1693. doi: 10.11895/j.issn.0253-3820.170300 shu

基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用

南京农业大学动物医学院, 南京 210095
基金项目:
本文系国家重点研发计划（Nos.2016YFD0501200，2016YFD0501009）、中央高校基本业务费（No.Y0201500195）、江苏省自然基金面上项目（No.BK20161452）、广东省科技计划重点项目（No.2017B020202010）和江苏高校优势学科建设工程资助项目资助

摘要: 以羧基化CdTe/ZnSe量子点荧光微球为标记物，通过1-乙基-（3-二甲基氨基丙基）碳二亚胺/N-羟基琥珀酰亚胺（EDC/NHS）活化法将氯霉素（CAP）单克隆抗体与量子点荧光微球偶联制备荧光探针。氯霉素全抗原（CAP-HS-BSA）及羊抗鼠二抗分别喷涂硝酸纤维素膜，形成检测线（T线）和质控线（C线），组装成新型氯霉素量子点荧光微球免疫层析试纸条，建立了快速、定量检测牛奶中CAP的方法。本研究开发的量子点荧光微球试纸条可在15 min内完成牛奶样品中CAP的定量检测，线性范围为0.1~100.0μg/L，检出限为0.1μg/L。牛奶样品CAP的加标回收率为93.3%~97.9%，相对标准偏差在4.9%~6.9%之间。

关键词:

English

Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Received Date: 09 May 2017
Revised Date: 04 September 2017
Fund Project:
This work was supported by the National Key R&D Program (Nos. 2016YFD0501200, 2016YFD0501009), the Fundamental Research Funds for the Central Universities (No. Y0201500195), the Natural Science Foundation of Jiangsu Province (No. BK20161452), the Key Program of Science and Technology Planning of Guangdong Province, China (No. 2017B020202010), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (Jiangsu, China).

Abstract: A fluorescent immunochromatographic test strip based on the quantum dots submicrobeads (QBs) was developed for quantitative detection of chloramphenicol (CAP). In this method, monoclonal antibody of CAP and OBs complex fluorescent probe was first prepared using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide coupling approach, then complete antigen CAP-HS-BSA was synthesized and sprayed on nitrocellulose membrane as test line (T line). Similarly, goat anti-mouse antibody was sprayed as control line (C line). The time required for the analysis was 15 min, and the limit of detection (LOD) for CAP was 0.1 μg/L, with a working range of 0.1-100 μg/L. In spiked milk samples, the test strip demonstrated high recoveries in the range from 93.3% to 97.9% with relative standard deviations of less than 7%.

Key words:

1. [1]
  Fraunfelder F T, Bagby G C, Kelly D J. Am. J. Ophthalmol., 1982, 93(3):420-422
2. [2]
  Kucers A. J. Antimicrob. Chemother., 1980, 6(1):1-4
3. [3]
  Samanidou V, Kehagia M, Kabir A, Furton K G. Analy. Chim. Acta, 2016, 914:62-74
4. [4]
  WU Li-Yuan.The Chinese Livestock and Poultry Breeding, 2014, 10(4):43-44 吴丽媛. 中国畜禽种业, 2014, 10(4):43-44
5. [5]
  YANG Cheng-Dui, SONG Li-Hui, MAO Li-Ha, LIU Mi-Xin.Chinese J. Anal. Chem., 2004, 32(7):905-907 杨成对, 宋莉晖, 毛利哈, 刘密新. 分析化学, 2004, 32(7):905-907
6. [6]
  Schwarm E F, Dabner C, Wilson J W, Boghosian M P. J. Pharmaceut. Sci., 1966, 55(7):744-745
7. [7]
  Anna Y K, Jeanne V S, Alexey M. Egorov. Food Agri. Immunol., 2000, 12:115-120
8. [8]
  ZHANG Xiao-Jun, ZHENG Bin. Chinese Journal of Analysis Laboratory, 2010, 29(6):115-118 张小军, 郑斌. 分析试验室, 2010, 29(6):115-118
9. [9]
  Huang X, Aguilar Z P, Xu H, Lai W, Xiong Y. Biosens. Bioelectron., 2016, 75:166-180
10. [10]
  Sakamoto S, Yusakul G, Nuntawong P, Kitisripanya T, Putalun W, Miyamoto T, Tanaka H, Morimoto S. J. Chromatogr. B, 2017, 1048:150-154
11. [11]
  Song C, Zhi A, Liu Q, Yang J, Jia G, Shervin J, Tang L, Hu X, Deng R, Xu C, Zhang G.Biosens. Bioelectron., 2013, 50:62-65
12. [12]
  Wang C, Hou F, Ma Y.Biosens. Bioelectron., 2015, 68:156-162
13. [13]
  Taranova N A, Berlina A N, Zherdev A V, Dzantiev B B.Biosens. Bioelectron., 2015, 63(2):255-261
14. [14]
  Di Nardo F, Anfossi L, Giovannoli C, Passini C, Goftman V V, Goryacheva I Y, Baggianic C. Talanta, 2016, 150:463-468
15. [15]
  DUAN Hong, CHEN Xue-Lan, JIANG Hu, SHEN Jun, DONG Sheng-Ming, XIONG Yong-Hua, Andrew Wang. Chinese J. Anal. Chem., 2015, 43(3):338-343 段宏, 陈学岚, 江湖, 沈骏, 董胜明, 熊勇华, Andrew Wang. 分析化学, 2015, 43(3):338-343
16. [16]
  ZHOU Yao-Feng,XIONG Si-Cheng, JIANG Hu, DUAN Hong, XIONG Yong-Hua. Chinese J. Anal. Chem., 2015, 43(12):1837-1843 周耀峰, 熊斯诚, 江湖, 段宏, 熊勇华. 分析化学, 2015, 43(12):1837-1843
17. [17]
  Campbell G S, Mageau R P, Schwab B, Johnston R W.Antimicrob. Agents Chemother., 1984, 25(2):205-211
18. [18]
  Kolosova A Y, Samsonova A M. Food Agri. Immunol., 2000, 12(2):115-125
19. [19]
  WANG Hong-Jiang, LIU Ting, XIE Ji, LI Wan-Yuan, GUO Ai-Ling, CAI Zhao-Xia.Chinese J. Anal. Chem., 2010, 38(5):632-637 王洪江, 柳婷, 谢跻, 栗婉媛, 郭爱玲, 蔡朝霞. 分析化学, 2010, 38(5):632-637
20. [20]
  XU Sheng-Nan,YU Qiong-Wei, YUAN Bi-Feng, FENG Yu-Qi. Journal of Analytical Science, 2016, 32(4):471-474 许胜男, 余琼卫, 袁必锋, 冯钰锜. 分析科学学报, 2016, 32(4):471-474
21. [21]
  XIAO Zhi-Li, ZENG Wen-Liang, WANG Hong, SHEN Yu-Dong, SUN Yuan-Ming, YANG Jin-Yi.Food & Machinery, 2013, 29(4):54-58 肖治理, 曾文亮,王弘, 沈玉栋,孙远明,杨金易. 食品与机械,2013, 29(4):54-58

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沈阳化工大学材料科学与工程学院沈阳 110142

基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用

English

Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于新型量子点荧光微球的氯霉素免疫层析试纸条的制备和应用

English

Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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