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Citation: CHEN Cheng, WANG Hong-Xi, KANG Hong-Jian, YAO Yao, QING Guang-Yan, LI Xiu-Ling, LIANG Xin-Miao. Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High-efficiency Glycopeptide Enrichment[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(8): 1149-1154. doi: 10.11895/j.issn.0253-3820.170165 shu

Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High-efficiency Glycopeptide Enrichment

1.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;2 State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China

Received Date: 17 March 2017
Revised Date: 27 May 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21475129)

In this study, a novel tetrapeptide hydrophilic interaction chromatography (HILIC) material was synthesized, and corresponding enrichment method for glycopeptides was developed. The tetrapeptide modified silica gel materials (denoted as Poly-DAPD) were synthesized by atom-transfer radical-polymerization (ATRP) and characterized by N₂ adsorption desorption and thermometer, thermal gravimetric analyzer (TGA) and X-ray photoelectron spectrometer (XPS). The characterization results indicated that tetrapeptide had been successfully synthesized on silica gel. Poly-DAPD materials showed high enrichment selectivity toward fetuin glycopeptides under solid-phase extraction (SPE) mode. Comparing with commercialized ZIC-HILIC in glycopeptides enrichment of fetuin digest which mixed with 5 mole ratio of albumin bovine (BSA), the as-prepared materials showed higher selectivity in both aspects of the identified number of glycopeptides and anti-interference property. The SPE results demonstrated that the tetrapeptide-based HILIC materials could be a potential tool in large-scale glycosylation analysis.
- Hydrophilic interaction chromatography,
- Tetrapeptide,
- Glycopeptide enrichment,
- Mass spectrometry
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