Citation: WEI Jia-Hui, WU Jian-Feng, CHEN Jia, WU Bi-Dong, CHEN Zuo-Hong, LIU Chang, XIE Jian-Wei. Identification and Content Difference of Toxic Peptides in 12 Kinds of Lethal Amanita Species[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(6): 817-823. doi: 10.11895/j.issn.0253-3820.170001 shu

Identification and Content Difference of Toxic Peptides in 12 Kinds of Lethal Amanita Species

1.
Pharmacy College, Jinzhou Medical University, Jinzhou, Liaoning 121000, China;
2.
State Key Laboratory of Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China;
3.
Colloge of life sciences, Hunan Normal University, Changsha 410081, China

Received Date: 1 January 2017
Revised Date: 31 March 2017

The types and relative content difference of the toxic peptides in twelve species of Amanita were evaluated by the high performance liquid chromatography (HPLC) and ultra performnace liquid chromatography-electrospray-quadrupole-time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS). The efficient toxic compounds in mushroom were extracted using 50% methanol in water containing 0.5% formic acid. Then, separation of various components in mushroom was carried out using Agilent 300 Extend-C₁₈ column with 0.05% (V/V) trifluoroacetic acid in 20 mmol/L ammonium acetate-methanol as mobile phase under a gradient program. The results indicated the significant difference in types and relative content of toxic peptides among all investigated mushrooms. In addition, we generated a chromatographic fingerprint based on the toxic peptide profiles. Finally 19 compositions were detected in the Amanita species, including 13 Amanita toxic peptides, 5 unknown toxic peptides and 1 unknown compound. These results play an important role in the discrimination of different Amanita species, and provide evidence in prevention and diagnosis of Amanitin poisoned events.
- Amanita species,
- Toxic peptides,
- Mass spectrometry,
- Identification,
- Chromatographic fingerprint
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