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Citation: LI Ai-Qin, GUO Chang, XU Su-Ying. A Novel Fluorescence Sensor for Highly Sensitive Detection of Glucose[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(6): 824-829. doi: 10.11895/j.issn.0253-3820.160855 shu

A Novel Fluorescence Sensor for Highly Sensitive Detection of Glucose

1.
Department of Gastroenterology, Chinese People's Liberation Army General Hospital, Beijing 100700, China;
2.
State Key Laboratory of Chemical Resource Engineering, Beijing Key Laboratory of Environmentally Harmful Chemical Analysis, Beijing University of Chemical Technology, Beijing 100029, China

Received Date: 23 November 2016
Revised Date: 3 May 2017

A fluorescence nanosensor based on an easily prepared fluorescent molecule, 1-oxo-1H-phenalene-2,3-dicarbonitrile (OPD), was developed for highly sensitive detection of glucose. Under the catalysis of horseradish peroxidase (HRP), 3,3',5,5'-tetramethylbenzidine (TMB) was oxidized into oxidized TMB (oxTMB) by H₂O₂. And the fluorescence of OPD was quenched by the intense absorption of the formed oxTMB, thus realizing effective quantitative detection of H₂O₂. The linear range was 0.05-0.8 μmol/L and 1-10 μmol/L respectively, with limit of detection of 0.02 μmol/L. Besides, on the basis of transformation of glucose into H₂O₂ through the catalysis of glucose oxidase, this nanosensor could be further exploited for highly sensitive detection of glucose. The TMB-HRP-OPD sensor exhibited linear range of 0.1-3.0 μmol/L and 4.0-30 μmol/L respectively for detection of glucose, with limit of detection of 0.02 μmol/L. Furthermore, it was successfully applied to the determination of glucose in real human serum and the results were in good agreement with the clinical data
- Organic fluorescent probes,
- Fluorescence nanosensor,
- Hydrogen peroxide,
- Glucose,
- Human serum
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