超滤法结合纳升液相色谱-串联质谱技术分析漏出性胸腔积液中多肽组分

引用本文: 陈世范, 杨静波, 张敏, 李晓鸥, 刘晓峰, 郭宏华, 何成彦, 高洪文. 超滤法结合纳升液相色谱-串联质谱技术分析漏出性胸腔积液中多肽组分[J]. 分析化学, 2017, 45(2): 224-230. doi: 10.11895/j.issn.0253-3820.160751 shu

Citation: CHEN Shi-Fan, YANG Jing-Bo, ZHANG Min, LI Xiao-Ou, LIU Xiao-Feng, GUO Hong-Hua, HE Cheng-Yan, GAO Hong-Wen. Peptidome Analysis of Transudative Pleural Effusion by Ultra-filtration Coupled with Nano-Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2017, 45(2): 224-230. doi: 10.11895/j.issn.0253-3820.160751 shu

超滤法结合纳升液相色谱-串联质谱技术分析漏出性胸腔积液中多肽组分

陈世范 ^1, ,
杨静波 ^1, ,
张敏 ^1, ,
李晓鸥 ^2, ,
刘晓峰 ^2, ,
郭宏华 ^3, ,
何成彦 ^3, ,
高洪文 ^1,

1.
吉林大学第二医院, 长春 130041;
2.
吉林省肿瘤医院, 长春 130021;
3.
吉林大学中日联谊医院, 长春 130041
基金项目:
本文系国家自然科学基金项目（Nos.81572082，81472030）、吉林省科技厅项目（Nos.20150101122JC，20150204001YY，20140311092YY）资助

摘要: 漏出性胸腔积液中的多肽和蛋白质等生物分子直接或间接地与机体特定的生理、病理状态相关，反映了肺部或者全身其它部位疾病的信息。本研究利用超滤法将漏出性胸腔积液中的多肽组分进行分离，经脱盐富集后，进行纳升液相色谱-串联质谱分析。结果表明，在漏出性胸腔积液中共鉴定到来源于52种蛋白的314条多肽，超过一半的肽段来源于纤维蛋白原，并且许多肽段具有阶梯序列的特征。此外，在来源于胶原蛋白和纤维蛋白原的多肽中还发现了大量的脯氨酸氧化修饰。基因本体论富集分析显示，漏出性胸腔积液多肽组分所属蛋白均具有胞外分泌的属性。本研究给出了漏出性胸腔积液中多肽组的序列、等电点、分子量、翻译后修饰等理化参数的分布特征，为进一步寻找肺部疾病相关的多肽标志物提供了可借鉴的参考数据和分析方法。

关键词:

English

Peptidome Analysis of Transudative Pleural Effusion by Ultra-filtration Coupled with Nano-Liquid Chromatography-Tandem Mass Spectrometry

1.
The Second Hospital of Jilin University, Changchun 130021, China;
2.
Tumor Hospital of Jilin Province, Changchun 130021, China;
3.
China-Japan Union Hospital, Jilin University, Changchun 130033, China
Received Date: 11 October 2016
Revised Date: 18 November 2016
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 81572082, 81472030) and Jilin Province Science and Technology Department (Nos. 2015101122JC, 20150204001YY, 20140311092YY)

Abstract: The peptides, proteins and other biological molecules in transudative pleural effusion correlate directly or indirectly with specific physiological and pathological state, reflecting the information regarding the lungs or other parts of the body. In the present study, the peptide fraction in transudative pleural effusion was isolated by ultrafiltration. After desalted and enriched by C₁₈ tips, the peptide mixture was analyzed by nano LC-MS/MS. The results showed that 314 peptides, which were originated from 52 proteins, in pleural transudate were identified. More than half of the peptides were derived from fibrinogen. Many peptides were characterized as displaying ladder sequences. In addition, a large number of proline oxidation modifications were detected in the peptides derived from collagen and fibrinogen. Gene ontology enrichment analysis showed that the most of the proteins extracellular properties of pleural transudate polypeptide components were protein with exocytosis. The study provided a rapid and efficient separation and analysis methods for lung disease markers related peptide compounds in pleural fluid leakage. Also this research provided a rapid and effective method for screening peptide biomarkers related to lung diseases from transudative pleural effusion.

Key words:

Peptidome
/ Ultrafiltration
/ Nano-liquid chromatography-tandem mass spectrometry
/ Transudative pleural effusion

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沈阳化工大学材料科学与工程学院沈阳 110142

超滤法结合纳升液相色谱-串联质谱技术分析漏出性胸腔积液中多肽组分

English

Peptidome Analysis of Transudative Pleural Effusion by Ultra-filtration Coupled with Nano-Liquid Chromatography-Tandem Mass Spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

超滤法结合纳升液相色谱-串联质谱技术分析漏出性胸腔积液中多肽组分

English

Peptidome Analysis of Transudative Pleural Effusion by Ultra-filtration Coupled with Nano-Liquid Chromatography-Tandem Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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