大豆苷元与氨基修饰<em>β-</em>环糊精包合物的制备、表征及水溶性

引用本文: 邓颖慧, 苏丽娜, 庞艳华, 郭亚飞, 王芬, 廖霞俐, 杨波. 大豆苷元与氨基修饰β-环糊精包合物的制备、表征及水溶性[J]. 分析化学, 2017, 45(5): 648-653. doi: 10.11895/j.issn.0253-3820.160747 shu

Citation: DENG Ying-Hui, SU Li-Na, PANG Yan-Hua, GUO Ya-Fei, WANG Fen, LIAO Xia-Li, YANG Bo. Preparation, Characterization and Water Solubility of Inclusion Complexes of Daidzein with Amino-modified β-Cyclodextrins[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 648-653. doi: 10.11895/j.issn.0253-3820.160747 shu

大豆苷元与氨基修饰β-环糊精包合物的制备、表征及水溶性

邓颖慧 ^1,2, ,
苏丽娜 ^1, ,
庞艳华 ^3, ,
郭亚飞 ^2, ,
王芬 ^2, ,
廖霞俐 ^2, ,
杨波 ^2,

1.
曲靖医学高等专科学校, 曲靖 655000;
2.
昆明理工大学生命科学与技术学院, 昆明 650500;
3.
武警总队曲靖支队, 曲靖 655000
基金项目:
本文系国家自然科学基金项目（No. 21362016）资助

摘要: 采用饱和水溶液法制备了大豆苷元分别与两种氨基修饰β-环糊精（ACD）即单-6-氨基-β-环糊精（NCD）和单-6-乙二胺基-β-环糊精（ENCD）的固体包合物，并获得最佳包合条件：大豆苷元与环糊精投料比为3∶1（n/n），搅拌时间为72 h，分别获得83%和67%的产率。利用X-射线粉末衍射和热重分析对其进行了表征，证实了两种包合物的形成。利用Job's曲线法确定了主客体的包合比为1∶1，并利用荧光光谱滴定分析测得其包合稳定常数K_S分别为899.2和203.8 L/mol。水溶性实验表明，通过与NCD和ENCD形成包合物，25℃下大豆苷元在水中的溶解度由原来的8.31 μg/mL增至15.2和13.2 mg/mL，分别提高了约1800和1500倍。

关键词:

English

Preparation, Characterization and Water Solubility of Inclusion Complexes of Daidzein with Amino-modified β-Cyclodextrins

1.
Qujing Medical College, Qujing 655000, China;
2.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China;
3.
Armed Police Yunnan Corps and Qujing Detachment, Qujing 655000, China
Received Date: 09 October 2016
Revised Date: 10 February 2017
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 21362016).

Abstract: To improve the water solubility of daidzein, solid inclusion complexes of daidzein with two amino-modified β-cyclodextrins (ACDs), i.e., mono-6-amino-6-deoxy-β-cyclodextrin (NCD) and mono-6-ethylenediamino-6-deoxy-β-cyclodextrin (ENCD), were prepared by the saturated solution method in water under the preparation conditions as follows: the ratio of daidzein/ACD was 3∶1 and the stirring time was 72 h (83% and 67% yields, respectively). The formation of two inclusion complexes was confirmed by x-ray diffractometry (XRD) and themogravimetric (TG) analysis. The inclusion stoichiometry of the inclusion complexes was 1∶1 from the Job plot and their complexation stability constants (K_S) were 899.2 and 203.8 L/mol from fluorescence titration, respectively. After formation of inclusion complexes with NCD and ENCD, the water solubility of daidzein was dramatically raised from 8.31 μg/mL to 15.2 and 13.2 mg/mL at 25℃, increasing by 1800-fold and 1500-fold.

Key words:

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大豆苷元与氨基修饰β-环糊精包合物的制备、表征及水溶性

English

Preparation, Characterization and Water Solubility of Inclusion Complexes of Daidzein with Amino-modified β-Cyclodextrins

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

大豆苷元与氨基修饰β-环糊精包合物的制备、表征及水溶性

English

Preparation, Characterization and Water Solubility of Inclusion Complexes of Daidzein with Amino-modified β-Cyclodextrins

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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