基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究

引用本文: 吴亮亮, 钱志娟, 谢正军, 张莹莹, 彭池方. 基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究[J]. 分析化学, 2017, 45(4): 471-476. doi: 10.11895/j.issn.0253-3820.160710 shu

Citation: WU Liang-Liang, QIAN Zhi-Juan, XIE Zheng-Jun, ZHANG Ying-Ying, PENG Chi-Fang. Colorimetric Detection of Copper Ions Based on Surface Modification of Silver/Platinum Cluster Nanoenzyme[J]. Chinese Journal of Analytical Chemistry, 2017, 45(4): 471-476. doi: 10.11895/j.issn.0253-3820.160710 shu

基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究

1.
江南大学, 食品科学与技术国家重点实验室, 食品学院, 无锡 214122;
2.
扬州出入境检验检疫局, 扬州 225000
基金项目:
本文系国家自然科学基金（No.31371767）、十二五国家科技支撑计划项目（No.2015BAD17B02）和江苏省出入境检验检疫局科技项目（No.2017KJ19）资助

摘要: 通过对银/铂纳米簇（Ag/Pt NCs）的表面修饰调控其催化活性，建立了一种高灵敏的比色法检测Cu²⁺。巯基丙酸能够抑制Ag/Pt NCs的催化活性，而巯基丙酸与Cu²⁺作用后，将导致上述抑制作用减弱。基于上述原理，通过测量Ag/Pt NCs 催化TMB-H₂O₂反应产生的显色信号，可以实现Cu²⁺的比色检测。本方法检测Cu²⁺的线性范围为10~100 nmol/L，检出限（3σ）为5.0 nmol/L。将本方法应用于实际水样中Cu²⁺的检测，结果表明，本方法具有操作简单、成本低、灵敏度高、特异性好等优点。

关键词:

English

Colorimetric Detection of Copper Ions Based on Surface Modification of Silver/Platinum Cluster Nanoenzyme

1.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
2.
Yangzhou Entry-Exit Inspection and Quarantine Bureau, Yangzhou 225000, China
Received Date: 21 September 2016
Revised Date: 21 November 2016
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.31371767) and the Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (No.2015BAD17B02).

Abstract: A sensitive colorimetric method for the detection of copper ions (Cu²⁺) was developed based on the surface modification of silver/platinum nanoclusters (Ag/Pt NCs) and regulation of peroxidase-like activity. It was found that 3-mercaptopropionic acid (MPA) could inhibit the catalytic ability of Ag/Pt NCs; however, it lost the inhibition toward catalytic ability of Ag/Pt NCs after oxidized by oxygen through the catalysis of Cu²⁺. On the basis of this, a colorimetric method was developed for the detection of Cu²⁺ through measuring the colorimetric signal variation of the TMB-H₂O₂ reaction. This method exhibited high sensitivity and selectivity toward Cu²⁺ over a panel of other metal ions. The linear range was 10-100 nmol/L and the detection limit was 5.0 nmol/L (3σ). The above method was also applied to detect real water samples and spiked samples, and the results demonstrated that this method was simple with low cost.

Key words:

1. [1]
  Wang S S, Wang X K, Zhang Z Y, Chen L X. Colloid Surf. A, 2015, 468: 333-338
2. [2]
  Zhang M, Ye B C. Analyst, 2011, 136(24): 5139-5142
3. [3]
  Valko M, Jomova K, Rhodes C J, Kuča K, Musílek K. Arch. Toxicol., 2016, 90(1): 1-37
4. [4]
  Amberger M A, Barth P, Förster O, Broekaert J A C. Microchim. Acta, 2011, 172(3): 261-267
5. [5]
  Qing Z, Mao Z, Qing T, He X, Zou Z, He D. Anal. Chem., 2014, 86(22): 11263-11268
6. [6]
  Djedjibegovic J, Larssen T, Skrbo A, Marjanovič A, Sober M. Food Chem., 2012, 131(2): 469-476
7. [7]
  Kopylovich M N, Mahmudov K T, Pombeiro A J L. J. Hazard. Mater., 2011, 186(2-3): 1154-1162
8. [8]
  Qiu S, Gao S, Zhu X, Lin Z, Qiu B, Chen G. Analyst, 2011, 136(8): 1580-1585
9. [9]
  Qiao Y, Zheng X. Analyst, 2015, 140(24): 8186-8193
10. [10]
  WANG Jia-Jia, LIAN Man, XIONG Jie, LI Zai-Jun. Chinese J. Anal. Chem., 2016, 44(3): 367-376 王佳佳, 连曼, 熊杰, 李在均. 分析化学, 2016, 44(3): 367-376
11. [11]
  Deng H H, Li G W, Liu A L, Chen W, Lin X H, Xia X H. Microchim. Acta, 2014, 181(9): 911-916
12. [12]
  Hua C, Zhang W H, De Almeida S R M, Ciampi S, Gloria D, Liu G. Analyst, 2012, 137(1): 82-86
13. [13]
  Sener G, Uzun L, Denizli A. Anal. Chem., 2014, 86(1): 514-520
14. [14]
  Lien C W, Chen Y C, Chang H T, Huang C C. Nanoscale, 2013, 5(17): 8227-8234
15. [15]
  Long Y J, Li Y F, Liu Y, Zheng J J, Tang J, Huang C Z. Chem. Commun., 2011, 47(43): 11939-11941
16. [16]
  Lien C W, Tseng Y T, Huang C C, Chang H T. Anal. Chem., 2014, 86(4): 2065-2072
17. [17]
  Ni P, Dai H, Wang Y, Sun Y, Shi Y, Hu J, Li Z. Biosens. Bioelectron., 2014, 60: 286-291
18. [18]
  Lin X Q, Deng H H, Wu G W, Peng H P, Liu A L, Lin X H. Analyst, 2015, 140(15): 5251-5256
19. [19]
  Gao Z, Xu M, Lu M, Chen G, Tang D. Biosens. Bioelectron., 2015, 70: 194-201
20. [20]
  Zheng C, Zheng A X, Liu B, Zhang X L, He Y, Li J, Yang H H, Chen G N. Chem. Commun., 2014, 50(86): 13103-13106
21. [21]
  Wu L L, Wang L Y, Xie Z J, Xue F, Peng C F. RSC Adv., 2016, 6(79): 75384-75389
22. [22]
  Wu L L, Wang L Y, Xie Z J, Pan N, Peng C F. Sens. Actuator B, 2016, 235: 110-116
23. [23]
  Bernardi F, Traverse A, Olivi L, Alves MCM, Morais J. J. Phys. Chem. C, 2011, 115(25): 12243-12249
24. [24]
  Fei S, Chen J, Yao S, Deng G, He D, Kuang Y. Anal. Biochem., 2005, 339(1): 29-35
25. [25]
  Pecci L, Montefoschi G, Musci G, Cavallini D. Amino Acids, 1997, 13(3): 355-367
26. [26]
  Tharmaraj V, Yang J. Analyst, 2014, 139(23): 6304-6309
27. [27]
  Liu R, Chen Z, Wang S, Qu C, Chen L, Wang Z. Talanta, 2013, 112: 37-42
28. [28]
  Yuan X, Chen Y. Analyst, 2012, 137(19): 4516-4523
29. [29]
  Mehta V N, Kumar M A, Kailasa S K. Indust. Engineer. Chem. Res., 2013, 52(12): 4414-4420
30. [30]
  Ma Y R, Niu H Y, Zhang X L, Cai Y Q. Chem. Commun., 2011, 47(47): 12643-12645
31. [31]
  Shen Q, Li W, Tang S, Hu Y, Nie Z, Huang Y, Yao S. Biosens. Bioelectron., 2013, 41: 663-668

扫一扫看文章

计量

PDF下载量: 13
文章访问数: 832
HTML全文浏览量: 63

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究

English

Colorimetric Detection of Copper Ions Based on Surface Modification of Silver/Platinum Cluster Nanoenzyme

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究

English

Colorimetric Detection of Copper Ions Based on Surface Modification of Silver/Platinum Cluster Nanoenzyme

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs