基于寡核苷酸链的汞离子荧光生物传感器

引用本文: 刘晨光, 王久军, 张兴平, 杨华林. 基于寡核苷酸链的汞离子荧光生物传感器[J]. 分析化学, 2017, 45(2): 163-168. doi: 10.11895/j.issn.0253-3820.160620 shu

Citation: LIU Chen-Guang, WANG Jiu-Jun, ZHANG Xing-Ping, YANG Hua-Lin. A Fluorescence Biosensor for Detection of Mercury Ion Based on Oligonucleotide[J]. Chinese Journal of Analytical Chemistry, 2017, 45(2): 163-168. doi: 10.11895/j.issn.0253-3820.160620 shu

基于寡核苷酸链的汞离子荧光生物传感器

长江大学生命科学学院, 荆州 434025
基金项目:
本文系国家自然科学基金（No.31601536）和湖北省大学生创新项目（No.20150091）资助

摘要: 基于G-四链体结构和卟啉类化合物N-甲基卟啉二丙酸IX（NMM）结合产生强烈的荧光，利用T-Hg（Ⅱ）-T错配对汞离子（Hg²⁺）的特异性识别，建立了一种简单、灵敏、高效的Hg²⁺检测新方法。在富含鸟嘌呤（G）寡核苷酸链中，引入了大量胸腺嘧啶（T）。在没有Hg²⁺存在时，可以自发形成G-四链体结构，与NMM结合产生强烈的荧光；在Hg²⁺存在时，可与另一条富含T序列的互补链通过T-Hg（Ⅱ）-T特异性结合，形成双链DNA分子，从而导致G-四链体结构不能产生。优化后最佳实验条件为：缓冲溶液的pH=6.7，20 mmol/L KCl，2.5 μmol/L NMM，反应时间为2 h。在优化条件下，体系的荧光强度变化值与Hg²⁺浓度呈现良好的线性关系，线性范围为50~1000 nmol/L，检出限为22.8 nmol/L（3σ）。此生物荧光传感器对Hg²⁺具有良好的选择性。实际水样中Hg²⁺的加标回收率为106.1%~107.8%，可以满足实际水样品中Hg²⁺的检测要求。

关键词:

English

A Fluorescence Biosensor for Detection of Mercury Ion Based on Oligonucleotide

College of College of Life Science, Yangtze University, Jingzhou 434025, China
Received Date: 22 August 2016
Revised Date: 17 November 2016
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 31601536)

Abstract: A simple, fast and highly sensitive fluorescence analysis method for detection of mercury ion was developed based on N-methyl-mesoporphyrin IX(NMM)/G-quadruplex DNA system and specific T-Hg(Ⅱ)-T mismatches. In this strategy, a large number of thymine was introduced into guanine-rich oigonucleotides which could form G-quadruplex. In the presence of Hg²⁺, guanine-rich oigonucleotides and complementary strand could form double-stranded DNA molecule by specific T-Hg(Ⅱ)-T mismatch pair, leading to destruction of G-quadruplex DNA structure. In the absence of Hg²⁺, guanine-rich oigonucleotides spontaneously formed G-quadruplex DNA structure that could bound NMM to generate intense fluorescence. Based on the above facts, a sensitive fluorescence biosensor for determination of Hg²⁺ was fabricated. And the optimal conditions for Hg²⁺ determination were as follows:buffer solution pH of 6.7, 20 mmol/L KCl and 2.5 μmol/L NMM in buffer and incubation for 2 h. Under the optimal conditions, the fluorescence intensity signal change (F₀-F) and the Hg²⁺ concentration exhibited a linear correlation within 50 nmol/L to 1000 nmol/L range with a low detection limit of 22.8 nmol/L (3σ). The biosensor exhibited good selectivity toward common metal ions. The developed method was successfully employed to detect Hg²⁺ in tap water with recovery of 106.1%-107.8%.

Key words:

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基于寡核苷酸链的汞离子荧光生物传感器

English

A Fluorescence Biosensor for Detection of Mercury Ion Based on Oligonucleotide

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中国化学会秘书处

基于寡核苷酸链的汞离子荧光生物传感器

English

A Fluorescence Biosensor for Detection of Mercury Ion Based on Oligonucleotide

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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