Citation: LIN Chun-Hua, LEI Zhi-Wei, LIU De-Yong, XU Zhao-Hui, LIAO Wei-Lin, SUN Jian-Song. Determination of Four Kinds of Monosaccharides and Disaccharides in Honey by Ultra Performance Convergence Chromatography with Evaporative Light Scattering Detector[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(12): 1912-1918. doi: 10.11895/j.issn.0253-3820.160476 shu

Determination of Four Kinds of Monosaccharides and Disaccharides in Honey by Ultra Performance Convergence Chromatography with Evaporative Light Scattering Detector

1.
National Monosaccharide Chemical Synthesis Engineering Research Center, Jiangxi Normal University, Nanchang 330027, China;
2.
Chemistry and Chemical Engineering Department, Jiangxi Normal University, Nanchang 330027, China

Received Date: 20 June 2016
Revised Date: 21 September 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21572081).

A method for determination of four monosaccharides and disaccharides in honey was established by ultra performance convergence chromatography coupled with evaporative light scattering detector (ELSD). The monosaccharides are fructose and glucose, and the disaccharides are sucrose and maltose. The chromatographic separation was conducted on an ACQUITY UPC² BEH column (100 mm×3.0 mm, 1.7 μm) using carbon dioxide and methanol (containing 0.2% ammonium hydroxide, V/V) as gradient elution. The flow rate was 1.0 mL/min with column temperature at 50℃. The drift tube temperature of the ELSD was set at 50℃ and the pressure of N₂ was set at 260.7 kPa. It was found that the reasonable linearity was achieved for monosaccharides and disaccharides with correlation coefficients not less than 0.9983. The limits of detection (S/N≥3) of the four saccharides were 2.0-4.7 mg/L. The recoveries for the four monosaccharides and disaccharides at three spiked levels were 86.6%-103.8% with relative standard deviations of 1.8%-4.3%. This method can be regarded as an excellent method for the determination of monosaccharides and disaccharides in honey samples, with the advantages of simplicity, rapidness, good separability and low cost. The detection result was consistent with that of national standard method.
- Ultra performance convergence chromatography,
- Evaporative light scattering detector,
- Honey,
- Monosaccharide,
- Disaccharide
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沈阳化工大学材料科学与工程学院沈阳 110142