Citation: HU Jing, GUO Yi-Rong, LIANG Xiao, LIU Xian-Jin, ZHU Guo-Nian, LIU Feng-Quan, WANG Ming-Hua, WANG Li-Min, HUA Xiu-De, ZHANG Cun-Zheng. Lateral Flow Immunoassay for Simultaneous Determination of Four Kinds of Pyrethroid Pesticides in Vegetables and Fruits[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(12): 1900-1906. doi: 10.11895/j.issn.0253-3820.160428 shu

Lateral Flow Immunoassay for Simultaneous Determination of Four Kinds of Pyrethroid Pesticides in Vegetables and Fruits

1.
Institute of Food Safety, Jiangsu Academy of Agricultural Science, Nanjing 210014, China;
2.
Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China;
3.
Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China;
4.
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China

Received Date: 3 June 2016
Revised Date: 31 August 2016

A monoclonal antibody based lateral flow immunoassay was developed for the simultaneous determination of four pyrethroid pesticides in vegetables and fruits, including fenpropathrin, cypermethrin, deltamethrin, and cyhalothrin. The colloidal gold particles (20-nm) were prepared by the method of sodium citrate reduction and conjugated with goat anti-mice IgG. Antigen and rabbit anti-goat IgG were immobilized on the nitrocellulose membrane as the test line (T line) and the control line (C line), separately. By sticking on PVC board, a gold immunochromatographic strip was assembled. About 10 g of samples was extracted by acetonitrile, and then diluted four times with PBS buffer. Anti-pyrethroid McAb was added in 100 μL of this extract solution for the antibody-antigen binding, thereafter, the mixture was ready for the strip testing. The results revealed that the test could be done within 10 min, and the cutoff values reached 0.5, 5.0, 5.0, 5.0 μg/mL for fenpropathrin, cypermethrin, deltamethrin, cyhalothrin, respectively. If the extraction going through QuEChERS method, the detection limit of method could be improved 16 times. Thereafter, the lateral flow immunoassay was employed for the verification test with 13 types of vegetables and fruits, except that the result of chillies presented the false positive result, the other results obtained higher consistency with the results of GC test. This new format of test strip using colloidal gold labeled goat anti-mice IgG as tracer, which could give more stable and repeatable results than classic test strip, would provide a new method for the simultaneous determination of four pyrethroid pesticides in vegetables and fruits.
- Pyrethroid pesticide,
- Lateral flow immunoassay,
- Rapid detection
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沈阳化工大学材料科学与工程学院沈阳 110142