Citation: DING Xiong, MU Ying. Research and Application Progress of Digital Nucleic Acid Amplification Detection Techniques[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(4): 512-521. doi: 10.11895/j.issn.0253-3820.160120 shu

Research and Application Progress of Digital Nucleic Acid Amplification Detection Techniques

DING Xiong ^1,2 ,
MU Ying ^1,,

1.
¹ Research Center for Analytical Instrumentation, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, China;
2.
² College of Life Sciences, Hangzhou, Zhejiang University, Hangzhou 310058, China

Corresponding author: MU Ying,
Received Date: 23 February 2016
Available Online: 18 March 2016
Fund Project: 本文系国家自然科学基金提供资助(No.31270907,31070772) (No.31270907,31070772)

Recently, micro/nano-scaled fluidic control technologies have been developed to be the alternative to traditional analysis approaches over several decades due to the capability of realizing miniaturized multiphase and multistep reactions. The perfect combination of these techniques and nucleic acid amplification methods effectively promoted the establishment and development of digital nucleic acid detection(dNAD) techniques. As a single-molecule analysis approach, dNAD played an essential role in molecular diagnosis. In this paper, the research and application progress of dNAD techniques were reviewed, including the development history, principle, superiority, and the future prospects of dNAD.
- Micro/nano-scal,
- Microfluidics,
- Digital nucleic acid amplification detection,
- Digital polymerase chain reaction,
- Fluidic control,
- Review
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