Citation: LI Jiu-Ling, SUN Kai, MENG Jia, SHEN Ji-Min, QI Hong, JIANG Lei. Detection of N-Acyl-homoserine Lactones Signal Molecules of Quorum Sensing Secreted by Denitrification Flora in Microaerobic Nitrogen Removal Processes by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(8): 1165-1170. doi: 10.11895/j.issn.0253-3820.160103 shu

Detection of N-Acyl-homoserine Lactones Signal Molecules of Quorum Sensing Secreted by Denitrification Flora in Microaerobic Nitrogen Removal Processes by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

LI Jiu-Ling ^1, ,
SUN Kai ¹ ,
MENG Jia ¹ ,
SHEN Ji-Min ¹ ,
QI Hong ¹ ,
JIANG Lei ²

1.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China;
2.
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100101, China

Received Date: 17 February 2016
Revised Date: 24 May 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 51478141)

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Gram-negative (G^-) bacteria, such as denitrifying bacteria and anaerobic ammonia oxidation bacteria, are highly social organisms capable of sophisticated cooperative behavior mediated via quorum sensing. As signal molecules of the chemical communication, N-acyl-homoserine lactones (AHLs) can mediate the quorum sensing of the functional microbial population and regulate the population density. To understand the growth of functional microbial population and the mechanism for biological nitrogen removal in upflow microaerobic sludge reactors (UMSRs) treating organic wastewater with low ratio of chemical oxygen demand to total nitrogen, a method was established to simultaneously detect AHLs in the microaerobic processes. Water-sludge mixtures sampled from the UMSRs were pretreated in sequence by liquid-liquid extraction using ethyl acetate, rotary evaporation, constant volume with methanol, separation by C18 column. Gradient elution was carried out using 5 mmol/L ammonium acetate (containing 0.1% formic acid) and methanol as mobile phases. On the base of multiple reaction monitoring analysis, a triple quadrupole mass spectrometer with an electrospray ionization was introduced to detect the target compounds. Nine kinds of AHLs were used to evaluate the established method and the results showed that the detection limits were 0.01-0.5 μg/L and all of the AHLs presented excellent linearity with the concentration ranging from 0.5 to 100 μg/L. The recovery and relative standard deviation ranged from 62.5% to 118.1% and 2.9% to 12.1%, respectively. The analysis could be finished within 6.5 min. The rapid, accurate and precise method for detecting AHLs provided a new insight into the growth and metabolic activity of functional microbial population in the activated sludge processes to understand the mechanism of biological nitrogen removal, suggesting a good application in regulation and operation of wastewater biological treatment processes.
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