Citation: TANG Jun, FU Qiang, CUI Meng, XING Jun-Peng, LIU Zhi-Qiang, LIU Shu-Ying. Interaction Studies of Lyszoyme and Flavonoids Using Intensity Fading-Matrix-assisted Laser Desorption/Ionization-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(7): 1071-1076. doi: 10.11895/j.issn.0253-3820.160078 shu

Interaction Studies of Lyszoyme and Flavonoids Using Intensity Fading-Matrix-assisted Laser Desorption/Ionization-Mass Spectrometry

TANG Jun ^1,2, ,
FU Qiang ^1,2 ,
CUI Meng ¹ ,
XING Jun-Peng ¹ ,
LIU Zhi-Qiang ¹ ,
LIU Shu-Ying ¹

1.
Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.
University of the Chinese Academy of Sciences, Beijing 100039, China

Received Date: 29 January 2016
Revised Date: 11 March 2016

Flavonoids are reported to have a variety of biological activities. Investigations on the interactions of flavonoids with protein are essential to understand the biological activities of flavnoids. In this paper, by using the optimized matrix 2,5-dihydroxybenzoic acid (DHB), we investigated the interaction of five flavonoids (luteolin, apigenin, genisten, quercetin, dazein) with lyszoyme using intensity fading (IF)-MALDI-MS method. The changes of relative abundances of flavonoids before and after addition of lyszoyme were calculated, and the binding affinity order was obtained by competitive experiments. It was found that all of the five flavonoids bound specifically to lysozyme. And the order of binding affinities of the five flavonoids to the lysozyme was luteolin>apigenin, genisten > quercetin > dazein. Based on their structures, it was found that the hydroxyl on C5 and C3' benefited the binding of flavonoid towards lyszoyme. However, the hydroxyl on C3 hindered the binding of flavonoid towards lysozyme. The results should be helpful to understand the biological activity of flavonoids. And the IF-MALDI-MS method could also be used to screening the interactions of other natural products and protein.
- Flavonoid,
- Lysozyme,
- Interaction,
- Matrix-assisted laser desorption/ionization
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