Citation: GONG Xue-Yong, ZHANG Hong-Zhi, HOU Chao, WANG Yu-Min, DONG Jian. Fluorescence Excitation-Emission Matrix Spectra Coupled with Physic and Mathematic Isolation to Study Composition of Dissolved Organic Matter[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(10): 1533-1538. doi: 10.11895/j.issn.0253-3820.160056 shu

Fluorescence Excitation-Emission Matrix Spectra Coupled with Physic and Mathematic Isolation to Study Composition of Dissolved Organic Matter

Institute of Chemical Engineering, Taishan Medical College, Tai'an 271016, China

Received Date: 20 January 2016
Revised Date: 20 July 2016

Fund Project: This work was supported by the National Natural Science Foundation of China. (Nos.21501130, 31370575)

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Combining with fluorescence excitation-emission matrix (EEM) spectra and parallel factor analysis, size exclusion chromatography (SEC) equipped with multi-excitation/emission scan model fluorescence detector was used for the analysis of the composition of dissolved organic matter (DOM) isolated from landfill leachates with different ages. The analytical results showed that the two leachate-derived DOMs both comprised protein- and humic-like substances. However, there were four kinds of protein-like matter in young landfill leachates, i.e., protein with high molecular weight, protein-like matter bound to humic-like substances with high or low molecular weight, and peptide/amino acids. While there were only two kinds of protein-like matter in old landfill leachates, i.e., protein with high molecular weight and protein-like matter bound to humic-like substances with high molecule weight. Compared with SEC, EEM spectra coupled with parallel factor analysis could identify the protein-like matter bound with humic-like substances or those presented as non-humic-like substances, though it could not identify the protein-like matter presented as protein and that presented as peptide/amino acids. The experimental results demonstrated that EEM spectra coupled with PARAFAC analysis and SEC could be used to characterize protein- and humic-like matter presented as different species.
- Dissolved organic matter,
- Fluorescence spectra,
- Size exclusion chromatography,
- Parallel factor analysis
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