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Citation: TAN Hua-Dong, LI Rui-Long, ZHU Ya-Xian, ZHANG Yong. In situ Determination of Anthracene Adsorbed onto Mangrove Root Surface Micro-zone Using Microscopic Fluorescence Spectrometric Analysis System[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(8): 1281-1285. doi: 10.11895/j.issn.0253-3820.160049 shu

In situ Determination of Anthracene Adsorbed onto Mangrove Root Surface Micro-zone Using Microscopic Fluorescence Spectrometric Analysis System

1.
State Key Laboratory of Marine Environmental Science of China (Xiamen University), College of the Environment and Ecology, Xiamen University, Xiamen 361002, China;
2.
Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Received Date: 18 January 2016
Revised Date: 19 April 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21577110, 21177102) and the Specialized Research Fund for the Doctoral Program of Higher Education of China (No.20130121130005)

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A microscopic fluorescence spectrometric analysis (MFSA) system was set in the laboratory. A novel method for in situ determination of Anthracene (Ant) adsorbed onto root surface micro-zone of two kinks of mangrove plant, named Kandelia obovata (K. obovata) and Avicennia marina (A. marina) by MFSA was established. Fluorescence spectra of Ant adsorbed on root surface micro-zone were obtained by synchronous scanning mode. The signal to noise (S/N) of Ant (5.3 pg/μm²) adsorbed on K. obovata and A. marina root surface micro-zone increased up to 5.5 and 6.8 while wavelength offset (Δλ) both were at 60 nm, respectively. The linear dynamic ranges of established method were 5.3-63.2 pg/μm² for K. obovata and 10.5-52.6 pg/μm² for A. marina, with the detection limits of 1.1 pg/μm² and 5.5 pg/μm², respectively. The relative standard deviations were both less than 12.5% (n=9), and the recoveries were 98.1%-117.0% and 81.2%-110.9%, respectively. The result showed that the MFSA system had ability to obtain quantitative information of fluorescence spectra and fluorescence image of Ant adsorbed onto plant roots surface micro-zone.
- Microscopic fluorescence spectrometric analysis,
- Anthracene,
- Root surface micro-zone,
- in situ
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