Citation: ZHUANG Qi-Chen, NING Rui-Zhi, MA Yuan, LIN Jin-Ming. Recent Development in Microfluidic Chips for in vitro Cell-based Research[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(4): 522-532. doi: 10.11895/j.issn.0253-3820.160003 shu

Recent Development in Microfluidic Chips for in vitro Cell-based Research

1.
¹ Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing 100084, China;
2.
² Department of Biomaterial Sciences, The University of Tokyo, Tokyo 113-8657, Japan

Corresponding author: LIN Jin-Ming,
Received Date: 3 January 2016
Available Online: 16 January 2016
Fund Project: 本文系国家自然科学基金(Nos.81373373,21227006,21435002) (Nos.81373373,21227006,21435002)国家"211工程"项目"高等学校仪器设备和优质资源共享系统"(No.CERS-1-75)资助 (No.CERS-1-75)

Microfluidic technology has become a powerful tool in cell-based research due to its inherent advantages. The research progress of cell culture on chips, simulation and manipulation of cell microenvironment on chips, single cell analysis on chips, organ-on-a-chip, and chip-mass spectrometry technology was introduced in this review. Morover, the summary and prospect on the microfluidic technology were introduced to give some insights for researchers who were interested in developing microfluidic platforms for cell-based research.
- Microfluidic chip,
- Cell culture,
- Microenvironment,
- Single cell,
- Organ-on-a-chip,
- Chip-mass spectrometry,
- Cell analysis,
- Review
1. [1]
  1 Yeo L, Chang H, Chan P, Friend J. Small, 2011, 7(1):12-48
2. [2]
  2 CHEN Li, ZHENG Xiao-Lin, HU Ning, YANG Jun, LUO Hong-Yan, JIANG Fan, LIAO Yan-Jian. Chinese J. Anal.Chem., 2015, 43(2):300-309 陈礼, 郑小林, 胡宁, 杨军, 罗洪艳, 姜帆, 廖彦剑. 分析化学, 2015, 43(2):300-309
3. [3]
  3 Meyvantsson I, Beebe D. Annu. Rev. Anal. Chem.(Palo.Alto. Calif.), 2008, 1:423-449
4. [4]
  4 Mao S, Gao D, Liu W, Wei H, Lin J M. Lab Chip, 2012, 12(1):219-226
5. [5]
  5 Gao D, Liu H, Lin J M, Wang Y, Jiang Y. Lab Chip, 2013, 13(5):978-985
6. [6]
  6 Wei H, Li H, Gao D, Lin J M. Analyst, 2010, 135(8):2043-2050
7. [7]
  7 Camelliti P, Mcculloch A D, Kohl P. Microsc. Microanal., 2005, 11(3):249-259
8. [8]
  8 Gao D, Wei H, Guo G S, Lin J M. Anal. Chem., 2010, 82(13):5679-5685
9. [9]
  9 Liu W, Wang N, Lin X, Ma Y, Lin J M. Anal. Chem., 2014, 86(14):7128-7134
10. [10]
  10 Vedel S, Tay S, Johnston D M, Bruus H, Quake S R. Proc. Natl. Acad. Sci. U. S. A., 2013, 110(1):129-134
11. [11]
  11 Huh D, Hamilton G A, Ingber D E. Trends. Cell Biol., 2011, 21(12):745-754
12. [12]
  12 Zheng C, Zhao L, Chen G, Zhou Y, Pang Y, Huang Y. Anal. Chem., 2012, 84(4):2088-2093
13. [13]
  13 Lecault V, Vaninsberghe M, Sekulovic S, Knapp D J, Wohrer S, Bowden W, Viel F, Mclaughlin T, Jarandehei A, Miller M, Falconnet D, White A K, Kent D G, Copley M R, Taghipour F, Eaves C J, Humphries R K, Piret J M, Hansen C L. Nat. Methods., 2011, 8(7):581-586
14. [14]
  14 Lii J, Hsu W J, Parsa H, Das A, Rouse R, Sia S K. Anal. Chem., 2008, 80(10):3640-3647
15. [15]
  15 Chung S, Sudo R, Mack P J, Wan C R, Vickerman V, Kamm R D. Lab Chip, 2009, 9(2):269-275
16. [16]
  16 Tumarkin E, Tzadu L, Csaszar E, Seo M, Zhang H, Lee A, Peerani R, Purpura K, Zandstra P W, Kumacheva E. Integr. Biol.(Camb.), 2011, 3(6):653-662
17. [17]
  17 Tsuda Y, Morimoto Y, Takeuchi S. Langmuir, 2010, 26(4):2645-2649
18. [18]
  18 Liu J, Gao D, Li H, Lin J M. Lab Chip, 2009, 9(9):1301-1305
19. [19]
  19 Wu J, Chen Q, Liu W, Zhang Y, Lin J M. Lab Chip, 2012, 12(18):3474-3480
20. [20]
  20 Ong S M, Zhang C, Toh Y C, Kim S H, Foo H L, Tan C H, van Noort D, Park S, Yu H. Biomaterials, 2008, 29(22):3237-3244
21. [21]
  21 Toh Y C, Zhang C, Zhang J, Khong Y M, Chang S, Samper V D, van Noort D, Hutmacher D W, Yu H. Lab Chip, 2007, 7(3):302-309
22. [22]
  22 Choi J, Kim S, Jung J, Lim Y, Kang K, Park S, Kang S. Biomaterials, 2011, 32(29):7013-7022
23. [23]
  23 Toh Y C, Lim T C, Tai D, Xiao G, van Noort D, Yu H. Lab Chip, 2009, 9(14):2026-2035
24. [24]
  24 Ravula S K, Wang M S, Asress S A, Glass J D, Bruno F A. J. Neurosci. Methods, 2007, 159(1):78-85
25. [25]
  25 Wong A P, Perez-Castillejos R, Christopher L J, Whitesides G M. Biomaterials, 2008, 29(12):1853-1861
26. [26]
  26 Kimura H, Yamamoto T, Sakai H, Sakai Y, Fujii T. Lab Chip, 2008, 8(5):741-746
27. [27]
  27 Yuan B, Li Y, Wang D, Xie Y, Liu Y, Cui L, Tu F, Li H, Ji H, Zhang W, Jiang X. Adv. Funct. Mater., 2010, 20(21):3715-3720
28. [28]
  28 Chen Q, Wu J, Zhuang Q, Lin X, Zhang J, Lin J M. Sci. Rep., 2013, 3:2433
29. [29]
  29 Zhuang Q, Wang S, Zhang J, He Z, Li H, Ma Y, Lin J M. Sci. China Chem., 2015, 58(8):1-8
30. [30]
  30 Mao S, Zhang J, Li H, Lin J M. Anal. Chem., 2013, 85(2):868-876
31. [31]
  31 Zhang J, Wu J, Li H, Chen Q, Lin J M. Biosens.Bioelectron., 2015, 68:322-328
32. [32]
  32 Wei H, Li H, Mao S, Lin J M. Anal. Chem., 2011, 83(24):9306-9313
33. [33]
  33 Gao D, Liu H, Jiang Y, Lin J M. TRAC-Trend Anal. Chem., 2012, 35:150-164
34. [34]
  34 Toh A G G, Wang Z P, Yang C, Nam-Trung N. Microfluid. Nanofluid., 2014, 16(1-2):1-18
35. [35]
  35 Jeon N L, Dertinger S, Chiu D T, Choi I S, Stroock A D, Whitesides G M. Langmuir, 2000, 16(22):8311-8316
36. [36]
  36 Gao D, Li H, Wang N, Lin J M. Anal. Chem., 2012, 84(21):9230-9237
37. [37]
  37 Chen Q, Wu J, Zhang Y, Lin J M. Anal. Chem., 2012, 84(3):1695-1701
38. [38]
  38 Scherber C, Aranyosi A J, Kulemann B, Thayer S P, Toner M, Iliopoulos O, Irimia D. Integr. Biol.-Uk., 2012, 4(3):259-269
39. [39]
  39 Byrne M B, Leslie M T, Gaskins H R, Kenis P J. Trends Biotechnol., 2014, 32(11):556-563
40. [40]
  40 Wang L, Liu W, Wang Y, Wang J C, Tu Q, Liu R, Wang J. Lab Chip, 2013, 13(4):695-705
41. [41]
  41 Lin X, Chen Q, Liu W, Zhang J, Wang S, Lin Z, Lin J M. Sci. Rep., 2015, 5:9643
42. [42]
  42 Rosso F, Giordano A, Barbarisi M, Barbarisi A. J. Cell Physiol., 2004, 199(2):174-180
43. [43]
  43 Wu J, Chen Q, Liu W, Lin J M. Lab Chip, 2013, 13(10):1948-1954
44. [44]
  44 Wu C, Asokan S B, Berginski M E, Haynes E M, Sharpless N E, Griffith J D, Gomez S M, Bear J E. Cell, 2012, 148(5):973-987
45. [45]
  45 Sun Y S, Peng S W, Cheng J Y. Biomicrofluidics, 2012, 6(3):34117
46. [46]
  46 Tong Z, Cheung L S, Stebe K J, Konstantopoulos K. Integr. Biol.(Camb.), 2012, 4(8):847-856
47. [47]
  47 Przybyla L M, Voldman J. Proc. Natl. Acad. Sci. U. S. A., 2012, 109(3):835-840
48. [48]
  48 Moledina F, Clarke G, Oskooei A, Onishi K, Gunther A, Zandstra P W. Proc. Natl. Acad. Sci. USA, 2012, 109(9):3264-3269
49. [49]
  49 YU Zhi-Long, HUANG Yan-Yi. Sci. China Chem., 2015, 45(11):1090-1101 虞之龙, 黄岩谊. 中国科学:化学, 2015, 45(11):1090-1101
50. [50]
  50 Chen Q, Wu J, Zhang Y, Lin Z, Lin J M. Lab Chip, 2012, 12(24):5180-5185
51. [51]
  51 Liu C, Liu J, Gao D, Ding M, Lin J M. Anal. Chem., 2010, 82(22):9418-9424
52. [52]
  52 Wei H, Chueh B H, Wu H, Hall E W, Li C W, Schirhagl R, Lin J M, Zare R N. Lab Chip, 2011, 11(2):238-245
53. [53]
  53 Wu J, Li H, Chen Q, Lin X, Liu W, Lin J M. RSC. Adv., 2014, 4(47):24929-24934
54. [54]
  54 Mach A J, Kim J H, Arshi A, Hur S C, Di Carlo D. Lab Chip, 2011, 11(17):2827-2834
55. [55]
  55 Wang B L, Ghaderi A, Zhou H, Agresti J, Weitz D A, Fink G R, Stephanopoulos G. Nat. Biotechnol., 2014, 32(5):473-478
56. [56]
  56 Huh D, Matthews B D, Mammoto A, Montoya-Zavala M, Hsin H Y, Ingber D E. Science, 2010, 328(5986):1662-1668
57. [57]
  57 Yeon J H, Ryu H R, Chung M, Hu Q P, Jeon N L. Lab Chip, 2012, 12(16):2815-2822
58. [58]
  58 Miller J S, Stevens K R, Yang M T, Baker B M, Nguyen D H, Cohen D M, Toro E, Chen A A, Galie P A, Yu X, Chaturvedi R, Bhatia S N, Chen C S. Nat. Mater., 2012, 11(9):768-774
59. [59]
  59 Song J W, Munn L L. Proc. Natl. Acad. Sci. USA, 2011, 108(37):15342-15347
60. [60]
  60 Ma Z, Liu Q, Liu H, Yang H, Yun J X, Eisenberg C, Borg T K, Xu M, Gao B Z. Lab Chip, 2012, 12(3):566-573
61. [61]
  61 Grosberg A, Alford P W, Mccain M L, Parker K K. Lab Chip, 2011, 11(24):4165-4173
62. [62]
  62 Lin X, Leung K H, Lin L, Lin L, Lin S, Leung C H, Ma D L, Lin J M. Biosens. Bioelectron., 2016, 79:41-47
63. [63]
  63 SU Wen-Tao, FENG Ke, QIN Jian-Hua. Chinese J. Anal. Chem., 2015, 43(10):1490-1498 苏文涛, 冯可, 秦建华. 分析化学, 2015, 43(10):1490-1498
64. [64]
  64 Lin X, Wu J, Li H, Wang Z, Lin J M. Talanta, 2013, 114:131-137
65. [65]
  65 Yi L, Xu X, Lin X, Li H, Ma Y, Lin J M. Analyst, 2014, 139:3330-3335
66. [66]
  66 LI Hai-Fang, ZHANG Ying, LIN Jin-Ming. Scientia Simia Chimica, 2014, 44(5):777-783 李海芳, 张英, 林金明. 中国科学:化学, 2014, 44(5):777-783
67. [67]
  67 Luo C, Ma Y, Li H, Chen F, Uchiyama K, Lin J M. J. Mass Spectrom., 2013, 48:321-328
68. [68]
  68 Liu W, Chen Q, Lin X, Lin J M. Analyst, 2015, 140:1551-1554
69. [69]
  69 Zhang Y, Li H, Ma Y, Lin J M. Sci. China Chem., 2014, 57(3):442-446
70. [70]
  70 He X, Chen Q, Zhang Y, Lin J M. TRAC-Trends Anal. Chem., 2014, 53:84-97
71. [71]
  71 Lin L, Lin J M. Journal of Pharmaceutical Analysis, 2015, 5(6):337-347
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