Citation: LIU Wei-Ping, LIN Jin-Qiong, DU Yan, QI Ming-Yue, LIANG Guang-Tie, YANG Na, LIU Da-Yu. Anti-Cancer Drug Testing Using Multi-Layer Paper-Supported Breast Cancer Tissue Array[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(4): 579-585. doi: 10.11895/j.issn.0253-3820.151025 shu

Anti-Cancer Drug Testing Using Multi-Layer Paper-Supported Breast Cancer Tissue Array

LIU Wei-Ping ¹ ,
LIN Jin-Qiong ¹ ,
DU Yan ¹ ,
QI Ming-Yue ¹ ,
LIANG Guang-Tie ^1,2 ,
YANG Na ^1,2 ,
LIU Da-Yu ^1,2,3,,

1.
¹ Department of Laboratory Medicine, Guangzhou First People's Hospital Affiliated Hospital of Guangzhou Medical University, Guangzhou 510180, China;
2.
² Clinical Molecular Medicine and Molecular Diagnosis Key Laboratory of Guangdong Province, Guangzhou 510180, China;
3.
³ Clinical Research Center, the Second Hospital Affiliated to Dalian Medical University, Dalian 116027, China

Corresponding author: LIU Da-Yu,
Received Date: 30 December 2015
Available Online: 5 February 2016
Fund Project: 本文系国家自然科学基金(Nos.81371649,81271730,81171418) (Nos.81371649,81271730,81171418)广州市医药卫生科技项目(Nos.20121A021002,20121A011035)资助 (Nos.20121A021002,20121A011035)

This paper describes a multi-layer paper-supported breast cancer tissue array. The paper-based microchip with arrayed hydrophilic spots was fabricated by wax printing. After cell seeding, the microchip was folded to form an artificial solid tumor, and the multilayer paper was covered by a layer of microporous membrane that mimics endothelial layer. After culturing the paper-supported breast cancer tissue for a certain period of time, the multi-layer device was unfold thus enabling detections of cell survival, proliferation, morphology and metabolism on different layers. Breast cancer cells cultured on paper had a survival rate >80% and aggregated into tumor spheroids. Intracellular lactic acid levels increased with the extension of culture time. Compared to two dimensional(2D) counterparts, the decreased fraction of S phase cell(15% vs 60%) was detected. The paper-supported breast cancer tissue showed a drug response mechanism similar to the tumor in vivo. Cancer cells within the tissue were less sensitive to doxorubicin and the IC₅₀ value detected was significantly higher than that obtained with 2D cultures(5μmol/L vs 1.14μmol/L). The multi-layer paper-supported breast cancer tissue array developed in this work featured simple tissue reconstruction and easy operation, thus providing an ideal tool for anti-cancer drug testing.
- Paper-based microfluidic chip,
- Breast cancer,
- Tissue microarray,
- Microenvironment,
- Anti-cancer drug
1. [1]
  1 Hatok J, Babusikova E, Matakova T, Mistuna D, Dobrota D, Racay P. Clin. Exp. Med., 2009, 9(1):1-7
2. [2]
  2 Damia G, Garattini S. Cancer Treatment Rev., 2014, 40(8):909-916
3. [3]
  3 Li X Y, Hu S Q, Xiao L. Eur. Rev. Med. Pharmacol. Sci., 2015, 19(11):2112-2119
4. [4]
  4 Kimlin L, Kassis J, Virador V. Expert Opin. Drug Discov., 2013, 8(12):1455-1466
5. [5]
  5 Greek R, Menache A. Int. J. Med. Sci., 2013, 10(3):206-221
6. [6]
  6 Mroue R, Bissell M J. Methods Mol. Biol., 2013, 945:221-250
7. [7]
  7 Nam K H, Smith A S, Lone S, Kwon S, Kim D H. J. Lab. Autom., 2015, 20(3):201-215
8. [8]
  8 Van D V, Trietsch S J, Joore J, Vulto P, Hankemeier T. Curr. Opin. Biotechnol., 2015, 35:118-126
9. [9]
  9 Schreiber-Brynzak E, Klapproth E, Unger C, Lichtscheidl-Schultz I, Göschl S, Schweighofer S, Keppler B K. Invest. New Drugs., 2015, 33(4):835-847
10. [10]
  10 Hirschhaeuser F, Menne H, Dittfeld C, West J, Mueller-Klieser W, Kunz-Schughart L A. J. Biotechnol., 2010, 148(1):3-15
11. [11]
  11 Kimura H, Ikeda T, Nakayama H, Sakai Y, Fujii T. J. Lab. Autom., 2015, 20(3):265-273
12. [12]
  12 Wang X, Kim H J, Xu P, Matsumoto A, Kaplan D L. Langmuir, 2005, 21(24):11335-11341
13. [13]
  13 Matsuzawa A, Matsusaki M, Akashi M. Langmuir, 2012, 29(24):7362-7368
14. [14]
  14 Sailer M, Sun K L W, Mermut O, Kennedy T E, Barrett C J. Biomaterials, 2012, 33(24):5841-5847
15. [15]
  15 Catros S, Guillemot F, Nandakumar A, Ziane S, Moroni L, Habibovic P, Fricain J C. Tissue Eng. Part C:Methods., 2011, 18(1):62-70
16. [16]
  16 Martinez A W, Phillips S T, Whitesides G M. Proc. Natl. Acad. Sci. USA., 2008, 105(50):19606-19611
17. [17]
  17 ZHANG Qiong, ZHOU Xiao-Mian, YAN Wei, LIANG Guang-Tie, ZHANG Qi-Chao, LIU Da-Yu. Chinese J. Anal. Chem., 2012, 40(7):996-1001 张琼, 周小棉, 严伟, 梁广铁, 张其超, 刘大渔. 分析化学, 2012, 40(7):996-1001
18. [18]
  18 Derda R, Laromaine A, Mammoto A, Tang S K, Mammoto T, Ingber D E, Whitesides G M. Proc. Natl. Acad. Sci. USA., 2009, 106(44):18457-18462
19. [19]
  19 Derda R, Tang S K, Laromaine A, Mosadegh B, Hong E, Mwangi M,Mammoto A, Ingber D E, Whitesides G M. PLoS One, 2011, 6(5):e18940
20. [20]
  20 Lu Y, Shi W, Qin J, Lin B. Anal. Chem., 2009, 82(1):329-335
21. [21]
  21 YAN Wei, ZHANG Qiong, CHEN Bin, LIANG Guang-Tie, LI Wei-Xuan, ZHOU Xiao-Mian, LIU Da-Yu. Chinese J. Anal. Chem., 2013, 41(6):822-827 严伟, 张琼, 陈斌, 梁广铁, 李伟萱, 周小棉, 刘大渔. 分析化学, 2013, 41(6):822-827
22. [22]
  22 Lee J S, Romero R, Han Y M, Kim H C, Kim C J, Hong J S, Huh D. J. Matern Fetal. Neonatal Med., 2015:1-9
23. [23]
  23 Vander Heiden M G, Canfley L C, Thompson C B. Science, 2009, 324(5930):1029-1033
24. [24]
  24 Parks S K, Chiche J, Pouyssegur J. J. Cell Physiol., 2011, 226(2):299-308
25. [25]
  25 Nolte D D, An R, Turek J, Jeong K. Biomed. Opt. Express., 2012, 3(11):2825-2841
26. [26]
  26 Elliott N T, Yuan F. J. Pharm. Sci., 2011, 100(1):59-74
1. [1]
  Jialin Xie , Qing Feng , Zhen Li , Jinyi Yang , Min Liu , Wei Qi . Life’s Guardian Angel: Progesterone. University Chemistry, 2024, 39(10): 416-419. doi: 10.12461/PKU.DXHX202403068
2. [2]
  Lin LI , Jiaxue LI , Meixia YANG , Jiayu DING , Jiaqi JING , Ruiping ZHANG . Preparation of mitoxantrone self-assembled carrier-free nanodrugs regulated by sodium acetate for apoptosis induction of human breast carcinoma cells. Chinese Journal of Inorganic Chemistry, 2025, 41(12): 2536-2548. doi: 10.11862/CJIC.20250138
3. [3]
  Jiahao Zeng , Hui Chao . 诱导程序性细胞死亡的金属抗肿瘤药物研究. University Chemistry, 2025, 40(6): 145-159. doi: 10.12461/PKU.DXHX202406019
4. [4]
  Qin Tu , Anju Tao , Tongtong Ma , Jinyi Wang . Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip. University Chemistry, 2024, 39(6): 271-277. doi: 10.3866/PKU.DXHX202309062
5. [5]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
6. [6]
  Zhibei Qu , Changxin Wang , Lei Li , Jiaze Li , Jun Zhang . Organoid-on-a-Chip for Drug Screening and the Inherent Biochemistry Principles. University Chemistry, 2024, 39(7): 278-286. doi: 10.3866/PKU.DXHX202311039
7. [7]
  Jian Li , Yu Zhang , Rongrong Yan , Kaiyuan Sun , Xiaoqing Liu , Zishang Liang , Yinan Jiao , Hui Bu , Xin Chen , Jinjin Zhao , Jianlin Shi . Highly Efficient, Targeted, and Traceable Perovskite Nanocrystals for Photoelectrocatalytic Oncotherapy. Acta Physico-Chimica Sinica, 2025, 41(5): 100042-0. doi: 10.1016/j.actphy.2024.100042
8. [8]
  Ping Song , Nan Zhang , Jie Wang , Rui Yan , Zhiqiang Wang , Yingxue Jin . Experimental Teaching Design on Synthesis and Antitumor Activity Study of Cu-Pyropheophorbide-a Methyl Ester. University Chemistry, 2024, 39(6): 278-286. doi: 10.3866/PKU.DXHX202310087
9. [9]
  Jing WU , Puzhen HUI , Huilin ZHENG , Pingchuan YUAN , Chunfei WANG , Hui WANG , Xiaoxia GU . Synthesis, crystal structures, and antitumor activities of transition metal complexes incorporating a naphthol-aldehyde Schiff base ligand. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2422-2428. doi: 10.11862/CJIC.20240278
10. [10]
  Hanxue LIU , Shijie LI , Meng REN , Xuling XUE , Hongke LIU . Design and antitumor properties of dehydroabietic acid functionalized cyclometalated iridium(Ⅲ) complex. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1483-1494. doi: 10.11862/CJIC.20250031
11. [11]
  Xiaotong LU , Pan ZHANG , Zijie ZHAO , Lei HUANG , Hongwei ZUO , Lili LIANG . Antitumor and antibacterial activities of pyridyl Schiff base indium and dysprosium complexes. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1523-1532. doi: 10.11862/CJIC.20250073
12. [12]
  Junjian Wang , Qingquan Yu , Shunyao Liu , Yuke Chen , Xiaoyu Liu , Guodong Li , Xiaoyan Liu , Hong Liu , Weijia Zhou . Laser-Induced Carbonization of Hydroxyapatite Sandwich Paper for Inkless Printing. Acta Physico-Chimica Sinica, 2024, 40(4): 2304024-0. doi: 10.3866/PKU.WHXB202304024
13. [13]
  Xiuya Ma , Yu Chen , Yan Zhang . Stories about Pharmaceuticals. University Chemistry, 2025, 40(7): 232-240. doi: 10.12461/PKU.DXHX202408003
14. [14]
  Yuping Wei , Yiting Wang , Jialiang Jiang , Jinxuan Deng , Hong Zhang , Xiaofei Ma , Junjie Li . Interdisciplinary Teaching Practice——Flexible Wearable Electronic Skin for Low-Temperature Environments. University Chemistry, 2024, 39(10): 261-270. doi: 10.12461/PKU.DXHX202404007
15. [15]
  Yuanyuan JIANG , Fangfang TU , Yuhong ZHANG , Shi CHEN , Jiayuan XIANG , Xinhui XIA . Preparation and electrochemical properties of high-stability cathode prelithiation additive. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1101-1111. doi: 10.11862/CJIC.20240441
16. [16]
  Ziheng Zhuang , Xiao Xu , Kin Shing Chan . Superdrugs for Superbugs. University Chemistry, 2024, 39(9): 128-133. doi: 10.3866/PKU.DXHX202309040
17. [17]
  Xiao Ma , Junjie Wang , Xin Chen , Jingcheng Li , Lihong Zhao , Xueping Sun , Shaojuan Cheng , Fang Wang . Exploring Innovative Approaches to Chemistry Instructional Organization Driven by Artificial Intelligence. University Chemistry, 2025, 40(9): 99-106. doi: 10.12461/PKU.DXHX202410085
18. [18]
  Nana Wang , Gaosheng Zhang , Huosheng Li , Tangfu Xiao . Discussion on the Teaching Reform of Environmental Functional Materials within the Context of “Double First-Class” Initiative: Emphasizing the Integration of Industry, Academia, Research, and Application. University Chemistry, 2024, 39(6): 137-144. doi: 10.3866/PKU.DXHX202312010
19. [19]
  Minwei Xie . Integrating Ideological and Political Education into Inorganic Chemistry: a Case on “Weak Acid Dissociation Equilibrium” in Environmental Science and Engineering. University Chemistry, 2025, 40(11): 24-30. doi: 10.12461/PKU.DXHX202412143
20. [20]
  Gaofeng Zeng , Shuyu Liu , Manle Jiang , Yu Wang , Ping Xu , Lei Wang . Micro/Nanorobots for Pollution Detection and Toxic Removal. University Chemistry, 2024, 39(9): 229-234. doi: 10.12461/PKU.DXHX202311055

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(528)
HTML views(47)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142