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Citation: CHEN Sheng-Lan, ZHAO Xiao-Yan, REN Lu-Jing, JI Xiao-Jun, HUANG He. Metabonomics Study on Enhancement of Unsaturated Fatty Acids Accumulation by Ammonium Sulfate in Schizochytrium sp.[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(5): 685-692. doi: 10.11895/j.issn.0253-3820.150948 shu

Metabonomics Study on Enhancement of Unsaturated Fatty Acids Accumulation by Ammonium Sulfate in Schizochytrium sp.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China

Received Date: 25 August 2015
Revised Date: 18 January 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21306085)

A metabonomics method based on gas chromatography-mass spectrometry (GC-MS) was established to analyze the changes of intracellular metabolites and study the mechanism of ammonium sulfate enhancing unsaturated fatty acids accumulation in Schizochytrium sp. Metabolites of cells cultivated at 0, 1.5, 2.5 and 3.5 g/L (NH₄)₂SO₄ were analyzed at different times. Heat map was obtained with K-Means algorithm for data clustering analysis. Principal component analysis (PCA) and partial least-squares discrimination analysis (OPLS-DA) were employed for pattern recognition, and an obvious distinction among different conditions was achieved. According to the LS-DA load diagram and variable important factor (VIP) value, 7 biomarkers were determined with significant differences between four different conditions, which were identified as glucose, inositol, mannitol, fructose, phosphoric acid, ethylamine and ethanolamine by searching in NIST and Wiley database. Metabolic pathway analysis indicated that the metabolic flux toward the Embden-Meyerh of-Parnas (EMP) pathway and acetic acid synthesis branch were weakened by ammonium sulfate. Meanwhile, malic enzyme activity increased and the accumulation of NADPH was also enhanced. These might be the reason for the improvement of polyunsaturated fatty acids.
- Schizochytrium sp,
- Metabonomics,
- Principal component analysis,
- Ammonium sulfate,
- Docosahexaenoic acid
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