Citation: LI Jing, LIU Xiao-Lei, ZHAO Xu, LI Wei, DAI Yu-Lin, YUE Hao. A Metabonomics Study of Childhood Pneumonia by Rapid Resolution Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(3): 451-455. doi: 10.11895/j.issn.0253-3820.150872 shu

A Metabonomics Study of Childhood Pneumonia by Rapid Resolution Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry

LI Jing ¹ ,
LIU Xiao-Lei ² ,
ZHAO Xu ³ ,
LI Wei ⁴ ,
DAI Yu-Lin ^2,, ,
YUE Hao ^2,,

1.
¹ Hospital Affiliated to Changchun University of Traditional Chinese Medicine, Changchun 130021, China;
2.
² JiLin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China;
3.
³ The First Hosital of Jilin University, Changchun 130021, China;
4.
⁴ Shenzhen Third People's Hospital, Shenzhen 518112, China

Corresponding author: DAI Yu-Lin, YUE Hao,
Received Date: 1 November 2015
Available Online: 23 November 2015
Fund Project: 本文系公益性行业(农业)专项(No.20130311106)资助 (农业)专项(No.20130311106)

In this study, the rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry (RRLC-QTOF/MS) was used to profile the metabolites of urine samples from Childhood Pneumonia (CP) patients and healthy controls and find the potential biomarkers which can support evidence to early diagnose and cure the disease. Choose 10 CP patients (age 47.72±2.35 months) and 10 healthy controls (age 46.65±1.97 months). The urine samples were analyzed by RRLC-QTOF/MS and then the resulting data matrices were analyzed by principal components analysis (PCA) to find the potential biomarkers. Urine samples of CP patients were successfully distinguished from those of healthy controls. A total of two significantly changed metabolites have been found and identified as potential biomarkers. It is suggested that the disorder of purine metabolism and amino acid metabolism may play an important role in the mechanism of CP.
- Childhood Pneumonia,
- Rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry,
- Uric acid,
- Metabonomics
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