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Citation: WANG Bo, ZHOU Wei, LIU Xiao-Hua, LIU Xiao-Ya, CHEN Ya-Li, FENG Shi-Lan. Rapid Separation and Determination of 5 Kinds Flavonoids in Astragali Radix Based on Ultra-performance Convergence Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(5): 731-739. doi: 10.11895/j.issn.0253-3820.150788 shu

Rapid Separation and Determination of 5 Kinds Flavonoids in Astragali Radix Based on Ultra-performance Convergence Chromatography

WANG Bo ^1,2, ,
ZHOU Wei ² ,
LIU Xiao-Hua ¹ ,
LIU Xiao-Ya ¹ ,
CHEN Ya-Li ¹ ,
FENG Shi-Lan ¹

1.
Department of Pharmacy, Lanzhou University, Lanzhou 730000, China;
2.
Central Laboratory of Technical Center of Gansu Entry-Exit Inspection and Quarantine Bureau, Lanzhou 730000, China

Received Date: 10 October 2015
Revised Date: 9 December 2015

Fund Project: This work was supported by the Key Project in National Science and Technology Pillar Program during the Twelfth Five Year Great Research Projects of (NO. 2011BAI05B02) of the Ministry of Science and Technology of China.

A method was developed for the rapid separation and determination of 5 kinds flavonoids (calycosin, calycosin-7-O-β-D-glucoside, ononin, medicarpin and formononetin) in Astragali Radix based on ultra-performance convergence chromatography. After extracting by 80% ethanol, the flavonoids were separated on a Waters Acquity UPC² CSH (100 mm×3.0 mm i.d., 1.8 μm) column at 40℃ by using supercritical CO₂-methanol (contain 0.2% H₃PO₄) acetonitrile as the mobile phase at a flow rate of 0.4 mL/min, and then analyzed by a UV detector at wavelength of 280 nm, the whole analysis progress was only 15 min. The results showed that the limits of detection (LOD) and the limits of quantitation (LOQ) of five flavonoids were between 0.3 and 0.5 mg/kg, and 1.0 and 2.0 mg/kg, respectively. The spiked recoveries were more than 99.7%, and the relative standard deviations (RSD) were less than 2.2% (n=6). Under the optimal conditions, 13 groups of Astragali Radix from different producing areas were detected. The contents of calycosin, calycosin-7-O-β-D-glucoside, medicarpin, ononin and formononetin were 4.8-102 mg/kg, 14-277 mg/kg, 0-135 mg/kg, and 5.3-119 mg/kg, 2.8-41 mg/kg, respectively. This method is simple, fast, accurate and reproducible, and the result is reliable. The method is applicable for the determination of 5 flavonoids in Astragali Radix.
- Ultra-performance convergence chromatography,
- Flavonoids,
- Astragali Radix
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