Citation: KONG Guang-Hui, LI Yong, PANG Tao, SHI Jun-Li. Quantitation of 18 Polyphenols in Tobacco Leaf Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2015, 43(12): 1913-1919. doi: 10.11895/j.issn.0253-3820.150706 shu

Quantitation of 18 Polyphenols in Tobacco Leaf Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
Yunnan Academy of Tobacco Agricultural Science, Yuxi 653100, China

Corresponding author: LI Yong,
Received Date: 6 September 2015
Available Online: 21 October 2015
Fund Project: 本文系云南省烟草专卖局基金资助项目(No.2014YN11) (No.2014YN11)

Polyphenols are very important secondary metabolites for tobacco plants. They are also considered as the important flavor precursors for cigarette industry. A liquid chromatography-tandem mass spectrometry(LC-MS/MS)-based method was established for the simultaneous determination of 18 polyphenols in tobacco leaf. The developed method can be used to determine 15 more polyphenols compared to the standard method for industry(YC/T 202-2006). Different kinds of extraction solution were compared and a solution of methanol-water-chloroform(5:2:2, V/V) was selected for the extraction because of its highest yield. Method validation was performed and the result showed that all the 18 polyphenols have their linear correlation coefficients(R²) more than 0.99. The low limit of determination and the low limit of quantitation were in the range of 1-150μg/L and 6-350μg/L, respectively. Intra-day and Inter-day reproducibility were in the range of 2.4%-6.5% and 4.5%-10.1%, respectively. Recoveries were in the range of 89.5%-103.7%. The established method was then successfully used to analyze polyphenols of tobacco leaves planted under different environment temperatures. The polyphenols can be classified in four groups based on their response to the temperature changing:the concentration increase with the increasing environment temperature, the concentration decrease with the increasing environment temperature, the concentration decrease with the environment temperature changes from the normal set point, and the concentration does not change significantly with the changing environment temperature.
- Tobacco,
- Polyphenols,
- Ultra performance liquid chromatography,
- Tandem mass spectrometry
1. [1]
  1 LIU Fang, YANG Liu, SUN Lin, ZHANG Xia, MIAO Ming-Ming, DING Zhong-Tao. Acta Tabac. Sin., 2008, 14(6):1-5 刘芳, 杨柳, 孙林, 张霞, 缪明明, 丁中涛. 中国烟草学报, 2008, 14(6):1-5
2. [2]
  2 PAN Rui-Zhi. Plant Physiology(4th ed.), Beijing:Higher Education Press, 2001:112-113 潘瑞炽. 植物生理学(第4版), 北京:高等教育出版社, 2001:112-113
3. [3]
  3 XU Zi-Cheng, QIN Lu, SHAO Hui-Fang, DAI Ya, LI Dong-Liang, TANG Shi-Jun. J. Henan Agric. Univ., 2010, 44(4):383-389 许自成, 秦璐, 邵惠芳, 戴亚, 李东亮, 唐士军. 河南农业大学学报, 2010, 44(4):383-389
4. [4]
  4 YU Jian-Jun, LI Lin, PANG Tian-He, REN Xiao-Hong, SHAO Hui-Fang. J. Henan Agric. Univ., 2006, 40(1):108-112 于建军, 李琳, 庞天河, 任晓红, 邵惠芳. 河南农业大学学报, 2006, 40(1):108-112
5. [5]
  5 YANG Zhi-Xiao, WANG Yi, REN Xue-Liang, LIU Hong-Feng, HAN Hui-Jie, ZHAO Jie-Hong. J. Henan Agric. Sci., 2012, 41(10):1-5 杨志晓, 王轶, 任学良, 刘红峰, 韩慧杰, 赵杰宏. 河南农业科学, 2012, 41(10):1-5
6. [6]
  6 LENG Hong-Qiong, GUO Ya-Dong, LIU Wei, ZHANG Tao, DENG Liang, SHEN Zhi-Qiang. Spectroscopy and Spectral Analysis, 2013, 33(7):1801-1804 冷红琼, 郭亚东, 刘巍, 张涛, 邓亮, 沈志强. 光谱学与光谱分析, 2013, 33(7):1801-1804
7. [7]
  7 LÜ Jing. Chinese. Tobac. Sci., 2014, 35(5):109-111 吕婧. 中国烟草科学, 2014, 35(5):109-111
8. [8]
  8 Li Z, Wang L, Yang G, Shi H, Jiang C, Liu W, Zhang Y. J. Chromatogr. Sci., 2003, 41(1):36-40
9. [9]
  9 Wang J, Lu D, Zhao H, Jiang B, Wang J, Ling X, Chai H, Ouyang P. J. Serb. Chem. Soc., 2010, 75(7):875-891
10. [10]
  10 Xie F, Yu A, Hou D, Liu H, Ding L, Zhang S. Am. J. Anal. Chem., 2011, 2(8):929-933
11. [11]
  11 CHEN Jian-Ming, FENG Hong-TAO, XIA Qi-DONG, ZHOU Gui-Yuan, LIU Jing, DONG Sheng-Qiang, YANG Shi-Hua. Anhui Agric. Sci., 2013, 41(13):5929-5930 陈剑明, 冯洪涛, 夏启东, 周桂园, 刘静, 董胜强, 杨式华. 安徽农业科学, 2013, 41(13):5929-5930
12. [12]
  12 CHEN Yan-Wu, XIAO Kun, YIN Du-Lin, LIAN Shi-Xun. J. Shunde Polytech., 2006, 4(1):27-30 陈燕舞, 肖坤, 尹笃林, 廉世勋. 顺德职业技术学院学报, 2006, 4(1):27-30
13. [13]
  13 LI Ying-Jin, LIU Yan-Hong, WU Yu-Ping, SHI Jun-Li, PANG Tao, KONG Guang-Hui. Chinese. J. Spectrosc. Lab., 2010, 27(6):2396-2401 李应金, 刘彦红, 吴玉萍, 师君丽, 逄涛, 孔光辉. 光谱实验室, 2010, 27(6):2396-2401
14. [14]
  14 SU Qiang, LIU Yang, GUO Wei-Min,LI Xu-Tao. Anhui Agric. Sci., 2010, 38(24):13083-13085 苏强, 刘阳, 过为民, 李许涛. 安徽农业科学, 2010, 38(24):13083-13085
15. [15]
  15 WU De-Xi, HE Jie, XIANG Li-Hong,WANG Chao, WU Wen-Dou, YANG Ying-Ming, LI Fan. Anhui Agric. Sci., 2014, 42(22):7561-7562 吴德喜, 何洁, 向丽红, 王超, 吴文斗, 杨应明, 李凡. 安徽农业科学, 2014, 42(22):7561-7562
16. [16]
  16 YANG Hong-Qi, WANG Yong, ZHOU Ji-Heng, ZHONG Ke-Jun, YUE Qian. Acta Tabac. Sin., 2007, 13(3):21-24 杨虹琦, 王勇, 周冀衡, 钟科军, 岳骞. 中国烟草学报, 2007, 13(3):21-24
17. [17]
  17 ZHUANG Ya-Dong, ZHU Huai-Yuan, CAO Yi, LIU Xian-Jun, SHEN Xiao-Chen. Tob. Sci. Technol., 2014,(6):38-41 庄亚东, 朱怀远, 曹毅, 刘献军, 沈晓晨. 烟草科技, 2014,(6):38-41
18. [18]
  18 Gu J, Zeng X, Kong B, Mao Y, Liu W, Wei W. Chromatogr., 2010, 71(9-10):769-774
19. [19]
  19 Zhao R, Li F, Hu J. Anal. Methods, 2011, 3(10):2421-2424
20. [20]
  20 Ji X, Wei Y, Liu G, Che H. J. Food Agric. Environ., 2013, 11(1):868-870
21. [21]
  21 Li Y, Pang T, Shi J, Lu X, Deng J, Lin Q. J. Sep. Sci., 2014, 37(21):3067-3073
22. [22]
  22 LI Yong, LIN Qian, PANG Tao, SHI Jun-Li. Chinese Journal of Chromatography, 2015, 33(7):746-752 李勇, 林茜, 逄涛, 师君丽. 色谱, 2015, 33(7):746-752
23. [23]
  23 BAI Chang-Min, ZHONG Ke-Jun, HUANG Jian-Guo, CAO Guo-Jun, XU Guo-Wang. Chinese J. Anal. Chem., 2006, 34(11):1619-162 白长敏, 钟科军, 黄建国, 曹国军, 许国旺. 分析化学, 2006, 34(11):1619-1621
24. [24]
  24 ZHANG Tian, DONG Xue-Chang, WU Fang-Ping, YANG Guang-Yu, QIAO Yong-Feng. Chinese J. Anal. Chem., 2005, 33(3):359-362 张甜, 董学畅, 吴方评, 杨光宇, 乔永峰. 分析化学, 2005, 33(3):359-362
1. [1]
  Zhenjun Mao , Haorui Gu , Haiyan Che , Xufeng Lin . Exploration on Experiment Teaching of UHPLC-IC Based on Valve Switching Method. University Chemistry, 2024, 39(4): 81-86. doi: 10.3866/PKU.DXHX202311013
2. [2]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
3. [3]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
4. [4]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
5. [5]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
6. [6]
  Mengyang LI , Hao XU , Zhonghao NIU , Chunhua GONG , Weihui ZHONG , Jingli XIE . Highly effective catalytic synthesis of β-amino alcohols by using viologen-polyoxometalate hybrid materials. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1294-1300. doi: 10.11862/CJIC.20250080
7. [7]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
8. [8]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
9. [9]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
10. [10]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
11. [11]
  Yu Peng , Jiawei Chen , Yue Yin , Yongjie Cao , Mochou Liao , Congxiao Wang , Xiaoli Dong , Yongyao Xia . Tailored cathode electrolyte interphase via ethylene carbonate-free electrolytes enabling stable and wide-temperature operation of high-voltage LiCoO₂. Acta Physico-Chimica Sinica, 2025, 41(8): 100087-0. doi: 10.1016/j.actphy.2025.100087
12. [12]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
13. [13]
  Yongqing Kuang , Jie Liu , Jianjun Feng , Wen Yang , Shuanglian Cai , Ling Shi . Experimental Design for the Two-Step Synthesis of Paracetamol from 4-Hydroxyacetophenone. University Chemistry, 2024, 39(8): 331-337. doi: 10.12461/PKU.DXHX202403012
14. [14]
  Yajie Li , Bin Chen , Yiping Wang , Hui Xing , Wei Zhao , Geng Zhang , Siqi Shi . Inhibiting Dendrite Growth by Customizing Electrolyte or Separator to Achieve Anisotropic Lithium-Ion Transport: A Phase-Field Study. Acta Physico-Chimica Sinica, 2024, 40(3): 2305053-0. doi: 10.3866/PKU.WHXB202305053
15. [15]
  Lu XU , Chengyu ZHANG , Wenjuan JI , Haiying YANG , Yunlong FU . Zinc metal-organic framework with high-density free carboxyl oxygen functionalized pore walls for targeted electrochemical sensing of paracetamol. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 907-918. doi: 10.11862/CJIC.20230431
16. [16]
  Zhongyan Cao , Shengnan Jin , Yuxia Wang , Yiyi Chen , Xianqiang Kong , Yuanqing Xu . Advances in Highly Selective Reactions Involving Phenol Derivatives as Aryl Radical Precursors. University Chemistry, 2025, 40(4): 245-252. doi: 10.12461/PKU.DXHX202405186
17. [17]
  Xiyuan Su , Zhenlin Hu , Ye Fan , Xianyuan Liu , Xianyong Lu . Change as You Want: Multi-Responsive Superhydrophobic Intelligent Actuation Material. University Chemistry, 2024, 39(5): 228-237. doi: 10.3866/PKU.DXHX202311059
18. [18]
  Zongpei Zhang , Yanyang Li , Yanan Si , Kai Li , Shuangquan Zang . Developing a Chemistry Experiment Center Employing a Multifaceted Approach to Serve High-Quality Laboratory Education. University Chemistry, 2024, 39(7): 13-19. doi: 10.12461/PKU.DXHX202404041
19. [19]
  Ruilin Han , Xiaoqi Yan . Comparison of Multiple Function Methods for Fitting Surface Tension and Concentration Curves. University Chemistry, 2024, 39(7): 381-385. doi: 10.3866/PKU.DXHX202311023
20. [20]
  Xingyuan Lu , Yutao Yao , Junjing Gu , Peifeng Su . Energy Decomposition Analysis and Its Application in the Many-Body Effect of Water Clusters. University Chemistry, 2025, 40(3): 100-107. doi: 10.12461/PKU.DXHX202405074

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(526)
HTML views(17)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142