石墨烯量子点-银纳米颗粒复合物用于过氧化氢和葡萄糖比色检测

引用本文: 夏畅, 海欣, 陈帅, 陈旭伟, 王建华. 石墨烯量子点-银纳米颗粒复合物用于过氧化氢和葡萄糖比色检测[J]. 分析化学, 2016, 44(1): 41-48. doi: 10.11895/j.issn.0253-3820.150568 shu

Citation: XIA Chang, HAI Xin, CHEN Shuai, CHEN Xu-Wei, WANG Jian-Hua. Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H₂O₂ and Glucose[J]. Chinese Journal of Analytical Chemistry, 2016, 44(1): 41-48. doi: 10.11895/j.issn.0253-3820.150568 shu

石墨烯量子点-银纳米颗粒复合物用于过氧化氢和葡萄糖比色检测

夏畅 ^1, ,
海欣 ^1, ,
陈帅 ^2, ,
陈旭伟 ^{1,
,} ,
王建华 ^{1,
,}

1.
¹ 东北大学理学院分析科学研究中心, 沈阳 110819;
2.
² 生命科学与健康学院, 沈阳 110819

通讯作者: 陈旭伟, 王建华; 陈旭伟, 王建华

基金项目:
本文系国家自然科学基金项目(Nos.21275027,21235001,21475017)资助 (Nos.21275027,21235001,21475017)

摘要: 以石墨烯量子点(GQDs)为还原剂和稳定剂,在其表面原位生长银纳米粒子(AgNPs),制备了具有良好分散性的GQDs/AgNPs纳米复合物,其粒径小于30 nm。GQDs/AgNPs纳米复合物具有类过氧化物酶的催化活性,能有效催化H₂O₂氧化3,3',5,5'-四甲基联苯胺(TMB)并发生显色反应。稳态动力学分析表明, GQDs/AgNPs催化动力学遵循典型的Michaelis-Menten模型,其催化机理符合乒乓机制。与辣根过氧化物酶(HRP)相比,GQDs/AgNPs纳米复合物具有更强的亲和性。基于GQDs/AgNPs的催化活性和葡萄糖氧化产生H₂O₂的原理,建立了H₂O₂和葡萄糖的比色检测方法,检出限分别为0.18和1.6 μmol/L。将本方法应用于血浆中葡萄糖的检测分析,结果与标准方法相符。

关键词:

English

Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H₂O₂ and Glucose

XIA Chang ^1, ,
HAI Xin ^1, ,
CHEN Shuai ^2, ,
CHEN Xu-Wei ^{1,
,} ,
WANG Jian-Hua ^{1,
,}

1.
¹ Research Center for Analytical Sciences, College of Sciences, Northeastern University, Shenyang 110819, China;
2.
² College of Life and Health Science, Northeastern University, Shenyang 110819, China

Corresponding author: 陈旭伟, 王建华; 陈旭伟, 王建华

Received Date: 16 July 2015
Fund Project:
本文系国家自然科学基金项目(Nos.21275027,21235001,21475017)资助

Abstract: Graphene quantum dots/Ag nanoparticles nanocomposite (GQDs/AgNPs) was prepared via in-situ growth of AgNPs on the surface of GQDs, in which GQDs served as both reducing agent and stabilizer. The as-prepared nanocomposites were mono-dispersed with an average diameter of less than 30 nm. The obtained GQDs@AgNPs nanocomposites exhibited excellent intrinsic peroxidese-like activity, which could catalyze the oxidization of 3,3',5,5'-tetramethylbenzidine (TMB) by H₂O₂ to produce a colour product. Steady-state kinetic assays showed that the catalytic activity of GQDs/AgNPs towards H₂O₂ fitted well with typical Michaelis-Menten kinetic model, followed by the ping-pong mechanism. Compared with the horseradish peroxidase (HRP), GQDs/AgNPs showed higher affinity towards TMB and H₂O₂ substrate. Based on the intrinsic peroxidese-like activity of GQDs/AgNPs nanocomposites and the production of H₂O₂ after the oxidation of glucose, a colorimetric method was developed for the detection of H₂O₂ and glucose, along with detection limit of 0.18 μmol/L and 1.6 μmol/L, respectively. The present method was applied to the detection of glucose in human serum, and the obtained results agreed with that obtained by reference method.

Key words:

Graphene quantum dots/Ag nanopartides nanocomposite
/ Peroxidase-like activity
/ Hydrogen peroxide
/ Glucose

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

石墨烯量子点-银纳米颗粒复合物用于过氧化氢和葡萄糖比色检测

通讯作者: 陈旭伟, 王建华; 陈旭伟, 王建华

English

Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H2O2 and Glucose

Corresponding author: 陈旭伟, 王建华; 陈旭伟, 王建华

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通讯作者: 陈斌, bchen63@163.com

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Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H₂O₂ and Glucose