石墨烯量子点-银纳米颗粒复合物用于过氧化氢和葡萄糖比色检测

引用本文: 夏畅, 海欣, 陈帅, 陈旭伟, 王建华. 石墨烯量子点-银纳米颗粒复合物用于过氧化氢和葡萄糖比色检测[J]. 分析化学, 2016, 44(1): 41-48. doi: 10.11895/j.issn.0253-3820.150568 shu

Citation: XIA Chang, HAI Xin, CHEN Shuai, CHEN Xu-Wei, WANG Jian-Hua. Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H₂O₂ and Glucose[J]. Chinese Journal of Analytical Chemistry, 2016, 44(1): 41-48. doi: 10.11895/j.issn.0253-3820.150568 shu

石墨烯量子点-银纳米颗粒复合物用于过氧化氢和葡萄糖比色检测

夏畅 ^1, ,
海欣 ^1, ,
陈帅 ^2, ,
陈旭伟 ^{1,
,} ,
王建华 ^{1,
,}

1.
¹ 东北大学理学院分析科学研究中心, 沈阳 110819;
2.
² 生命科学与健康学院, 沈阳 110819

通讯作者: 陈旭伟, 王建华; 陈旭伟, 王建华

基金项目:
本文系国家自然科学基金项目(Nos.21275027,21235001,21475017)资助 (Nos.21275027,21235001,21475017)

摘要: 以石墨烯量子点(GQDs)为还原剂和稳定剂,在其表面原位生长银纳米粒子(AgNPs),制备了具有良好分散性的GQDs/AgNPs纳米复合物,其粒径小于30 nm。GQDs/AgNPs纳米复合物具有类过氧化物酶的催化活性,能有效催化H₂O₂氧化3,3',5,5'-四甲基联苯胺(TMB)并发生显色反应。稳态动力学分析表明, GQDs/AgNPs催化动力学遵循典型的Michaelis-Menten模型,其催化机理符合乒乓机制。与辣根过氧化物酶(HRP)相比,GQDs/AgNPs纳米复合物具有更强的亲和性。基于GQDs/AgNPs的催化活性和葡萄糖氧化产生H₂O₂的原理,建立了H₂O₂和葡萄糖的比色检测方法,检出限分别为0.18和1.6 μmol/L。将本方法应用于血浆中葡萄糖的检测分析,结果与标准方法相符。

关键词:

English

Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H₂O₂ and Glucose

XIA Chang ^1, ,
HAI Xin ^1, ,
CHEN Shuai ^2, ,
CHEN Xu-Wei ^{1,
,} ,
WANG Jian-Hua ^{1,
,}

1.
¹ Research Center for Analytical Sciences, College of Sciences, Northeastern University, Shenyang 110819, China;
2.
² College of Life and Health Science, Northeastern University, Shenyang 110819, China

Corresponding author: 陈旭伟, 王建华; 陈旭伟, 王建华

Received Date: 16 July 2015
Fund Project:
本文系国家自然科学基金项目(Nos.21275027,21235001,21475017)资助

Abstract: Graphene quantum dots/Ag nanoparticles nanocomposite (GQDs/AgNPs) was prepared via in-situ growth of AgNPs on the surface of GQDs, in which GQDs served as both reducing agent and stabilizer. The as-prepared nanocomposites were mono-dispersed with an average diameter of less than 30 nm. The obtained GQDs@AgNPs nanocomposites exhibited excellent intrinsic peroxidese-like activity, which could catalyze the oxidization of 3,3',5,5'-tetramethylbenzidine (TMB) by H₂O₂ to produce a colour product. Steady-state kinetic assays showed that the catalytic activity of GQDs/AgNPs towards H₂O₂ fitted well with typical Michaelis-Menten kinetic model, followed by the ping-pong mechanism. Compared with the horseradish peroxidase (HRP), GQDs/AgNPs showed higher affinity towards TMB and H₂O₂ substrate. Based on the intrinsic peroxidese-like activity of GQDs/AgNPs nanocomposites and the production of H₂O₂ after the oxidation of glucose, a colorimetric method was developed for the detection of H₂O₂ and glucose, along with detection limit of 0.18 μmol/L and 1.6 μmol/L, respectively. The present method was applied to the detection of glucose in human serum, and the obtained results agreed with that obtained by reference method.

Key words:

Graphene quantum dots/Ag nanopartides nanocomposite
/ Peroxidase-like activity
/ Hydrogen peroxide
/ Glucose

1. [1]
  1 Wang Y, Dai C, Yan X P. Chem. Commun., 2014,50(92):14341-143441 Wang Y, Dai C, Yan X P. Chem. Commun., 2014,50(92):14341-14344
2. [2]
  2 Jeon E K, Seo E, Lee E, Lee W, Um M K, Kim B S. Chem. Commun., 2013,49(33):3392-33942 Jeon E K, Seo E, Lee E, Lee W, Um M K, Kim B S. Chem. Commun., 2013,49(33):3392-3394
3. [3]
  3 Zhou Y Z, Cheng X N, Yang J, Zhao N, Ma S B, Li D, Zhong T. RSC Adv., 2013,3(45):23236-232413 Zhou Y Z, Cheng X N, Yang J, Zhao N, Ma S B, Li D, Zhong T. RSC Adv., 2013,3(45):23236-23241
4. [4]
  4 Wu M L, Lu D B, Zhao Y, Ju T Z. Micro Nano Lett., 2013,8(2):82-854 Wu M L, Lu D B, Zhao Y, Ju T Z. Micro Nano Lett., 2013,8(2):82-85
5. [5]
  5 Tang X Z, Cao Z W, Zhang H B, Liu J, Yu Z Z. Chem. Commun., 2011, 47(11):3084-30865 Tang X Z, Cao Z W, Zhang H B, Liu J, Yu Z Z. Chem. Commun., 2011, 47(11):3084-3086
6. [6]
  6 Yang L, Luo W, Cheng G Z. ACS Appl. Mater. Interfaces, 2013,5(16):8231-82406 Yang L, Luo W, Cheng G Z. ACS Appl. Mater. Interfaces, 2013,5(16):8231-8240
7. [7]
  7 Sun Z Y, Dong N N, Wang K P. K nig D, Nagaiah T C, Sánchez M D, Ludwig A, Cheng X, Schuhmann W G, Wang J, Muhler M. Carbon, 2013,62:182-1927 Sun Z Y, Dong N N, Wang K P. K nig D, Nagaiah T C, Sánchez M D, Ludwig A, Cheng X, Schuhmann W G, Wang J, Muhler M. Carbon, 2013,62:182-192
8. [8]
  8 WU Ling, CAO Zhong, SONG Tian-Ming, SONG Cheng, XIE Jing-Lei, HE Jing-Lin, XIAO Zhong-Liang. Chinese J. Anal. Chem., 2014,42(11):1656-1660 吴玲, 曹忠, 宋天铭, 宋铖, 谢晶磊, 何婧琳, 肖忠良.分析化学,2014,42(11):1656-16608 WU Ling, CAO Zhong, SONG Tian-Ming, SONG Cheng, XIE Jing-Lei, HE Jing-Lin, XIAO Zhong-Liang. Chinese J. Anal. Chem., 2014,42(11):1656-1660 吴玲, 曹忠, 宋天铭, 宋铖, 谢晶磊, 何婧琳, 肖忠良.分析化学,2014,42(11):1656-1660
9. [9]
  9 Zhang Y M, Yuan X, Wang Y, Chen Y. J. Mater. Chem., 2012, 22(15):7245-72519 Zhang Y M, Yuan X, Wang Y, Chen Y. J. Mater. Chem., 2012, 22(15):7245-7251
10. [10]
  10 WANG Lu, ZANG Xiao-Huan, WANG Chun, WANG Zhi. Chinese J. Anal. Chem., 2014,42(1):136-144 王璐, 臧晓欢, 王春, 王志.分析化学,2014,42(1):136-14410 WANG Lu, ZANG Xiao-Huan, WANG Chun, WANG Zhi. Chinese J. Anal. Chem., 2014,42(1):136-144 王璐, 臧晓欢, 王春, 王志.分析化学,2014,42(1):136-144
11. [11]
  11 Li L L, Wu G H,Yang G H, Peng J, Zhao J W, Zhu J J. Nanoscale, 2013,5(10):4015-403911 Li L L, Wu G H,Yang G H, Peng J, Zhao J W, Zhu J J. Nanoscale, 2013,5(10):4015-4039
12. [12]
  12 Yan X, Li B, Li L S. Accounts Chem. Res., 2013, 46(10):2254-226212 Yan X, Li B, Li L S. Accounts Chem. Res., 2013, 46(10):2254-2262
13. [13]
  13 Lin L P, Rong M C, Luo F, Chen D M, Wang Y R, Chen X. TRAC-Trend Anal. Chem., 2014,54:83-10213 Lin L P, Rong M C, Luo F, Chen D M, Wang Y R, Chen X. TRAC-Trend Anal. Chem., 2014,54:83-102
14. [14]
  14 Hummers Jr W S, Offeman R E. J. Am. Chem. Soc., 1958,80(6):1339-133914 Hummers Jr W S, Offeman R E. J. Am. Chem. Soc., 1958,80(6):1339-1339
15. [15]
  15 Wei Z Q, Wang D, Kim S, Hu Y, Yakes M K, Laracuente A R, Dai Z, Marder S R, Berger C, King W P, Heer W A, Sheehan P E, Riedo E. Science, 2010,328(5984):1373-137615 Wei Z Q, Wang D, Kim S, Hu Y, Yakes M K, Laracuente A R, Dai Z, Marder S R, Berger C, King W P, Heer W A, Sheehan P E, Riedo E. Science, 2010,328(5984):1373-1376
16. [16]
  16 YU Mei, LIU Peng-Rui, SUN Yu-Jing, LIU Jian-Hua, AN Jun-Wei, LI Song-Mei. Chinese J. Inorg. Mater., 2012,27(1):89-94 于美, 刘鹏瑞, 孙玉静, 刘建华, 安军伟, 李松梅.无机材料学报,2012,27(1):89-9416 YU Mei, LIU Peng-Rui, SUN Yu-Jing, LIU Jian-Hua, AN Jun-Wei, LI Song-Mei. Chinese J. Inorg. Mater., 2012,27(1):89-94 于美, 刘鹏瑞, 孙玉静, 刘建华, 安军伟, 李松梅.无机材料学报,2012,27(1):89-94
17. [17]
  17 Ran X, Sun H, Pu F, Ren J-S, Qu X-G. Chem. Commun., 2013,49(11):1079-108117 Ran X, Sun H, Pu F, Ren J-S, Qu X-G. Chem. Commun., 2013,49(11):1079-1081
18. [18]
  18 Liu M, Zhao H M, Chen S, Yu H T, Quan X. ACS Nano, 2012,3(6):3142-315118 Liu M, Zhao H M, Chen S, Yu H T, Quan X. ACS Nano, 2012,3(6):3142-3151
19. [19]
  19 Gao L Z, Zhuang J, Nie L, Zhang J B, Zhang Y, Gu N, Wang T H, Feng J, Yang D L, Perrett S. Nat. Nanotechnol., 2007,2(9):577-58319 Gao L Z, Zhuang J, Nie L, Zhang J B, Zhang Y, Gu N, Wang T H, Feng J, Yang D L, Perrett S. Nat. Nanotechnol., 2007,2(9):577-583
20. [20]
  20 XIE Jian-Xin. Chracteristics of Nanomaterials as Peroxidase Mimetics and Their Analytical Applications, Southeast University, Chongqing, 2012 谢建新.纳米材料过氧化物模拟酶特性及其应用研究, 重庆:西南大学,201220 XIE Jian-Xin. Chracteristics of Nanomaterials as Peroxidase Mimetics and Their Analytical Applications, Southeast University, Chongqing, 2012 谢建新.纳米材料过氧化物模拟酶特性及其应用研究, 重庆:西南大学,2012
21. [21]
  21 Eisenmesser E Z, Bosco D A, Akke M, Kern D. Science, 2002,295(5559):1520-152321 Eisenmesser E Z, Bosco D A, Akke M, Kern D. Science, 2002,295(5559):1520-1523
22. [22]
  22 Lin L P, Song X H, Chen Y Y, Rong M C, Zhao T T, Wang Y R, Jiang Y Q, Chen X. Anal. Chim. Acta, 2015,869:89-9522 Lin L P, Song X H, Chen Y Y, Rong M C, Zhao T T, Wang Y R, Jiang Y Q, Chen X. Anal. Chim. Acta, 2015,869:89-95
23. [23]
  23 Xie J X, Cao H Y, Jiang H, Chen Y J, Shi W B, Zheng H Z, Huang Y M. Anal. Chim. Acta, 2013,796:92-10023 Xie J X, Cao H Y, Jiang H, Chen Y J, Shi W B, Zheng H Z, Huang Y M. Anal. Chim. Acta, 2013,796:92-100
24. [24]
  24 Dong Y L, Zhang H G, Rahman Z U, Su L, Chen X J, Hu J, Chen X G. Nanoscale, 2012,4(13):3969-397624 Dong Y L, Zhang H G, Rahman Z U, Su L, Chen X J, Hu J, Chen X G. Nanoscale, 2012,4(13):3969-3976
25. [25]
  25 Hao J H, Zhang Z, Yang W S, Lu B P, Ke X, Zhang B L, Tang J L. J. Mater. Chem. A, 2013,1:4352-435725 Hao J H, Zhang Z, Yang W S, Lu B P, Ke X, Zhang B L, Tang J L. J. Mater. Chem. A, 2013,1:4352-4357

扫一扫看文章

计量

PDF下载量: 33
文章访问数: 613
HTML全文浏览量: 82

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

石墨烯量子点-银纳米颗粒复合物用于过氧化氢和葡萄糖比色检测

通讯作者: 陈旭伟, 王建华; 陈旭伟, 王建华

English

Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H₂O₂ and Glucose

Corresponding author: 陈旭伟, 王建华; 陈旭伟, 王建华

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

石墨烯量子点-银纳米颗粒复合物用于过氧化氢和葡萄糖比色检测

通讯作者: 陈旭伟, 王建华; 陈旭伟, 王建华

English

Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H2O2 and Glucose

Corresponding author: 陈旭伟, 王建华; 陈旭伟, 王建华

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

Preparation of Graphene Quantum Dots/Ag Nanoparticles Nanocomposites for Colorimetric Detection of H₂O₂ and Glucose

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs