Citation: LIN Chun-Hua, LIU De-Yong, FAN Nai-Li, XIA Jian-Hui, LIAO Wei-Lin. Determination of Main Composition of Fatty Acid Monoglyceride in Monoglyceride Emulsifier Using Ultra Performance Convergence Chromatography-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 281-288. doi: 10.11895/j.issn.0253-3820.150558 shu

Determination of Main Composition of Fatty Acid Monoglyceride in Monoglyceride Emulsifier Using Ultra Performance Convergence Chromatography-Mass Spectrometry

1.
¹ National Monosaccharide Chemical Synthesis Engineering Research Center, Jiangxi Normal University, Nanchang 330027, China;
2.
² Chemistry and Chemical Engineering Department, Jiangxi Normal University, Nanchang 330027, China

Corresponding author: XIA Jian-Hui,
Received Date: 14 July 2015
Available Online: 21 October 2015
Fund Project: 本文系国家科技支撑计划项目(Nos.2012BAE07B00,2014BAE13B02)资助 (Nos.2012BAE07B00,2014BAE13B02)

A fast analytical method was developed for the determination of main composition of fatty acid monoglycerides, including monopalmitin, monostearin, monoolein and monolinolein, in monoglycerede emulsifiers by ultra-performance convergence chromatography-mass spectrometry. Their contents were compared in the 3 emulsifiers from different sources. The sample was directly dissolved with n-hexane/isopropanol (7:3, /V) The chromatographic separation was performed on the ACQUITY UPC² BEH 2-EP column (2.1 mm×100 mm, 1.7 μm) using the mobile phases of carbon dioxide and methanol/acetonitrile (1:1, V/V) solution with gradient elution. The separated compounds were detected by MS detector in positive electrospray ionization (ESI⁺) and quantified by external standard method. The results showed that the calibration curves of monopalmitin,monostearin,monoolein and monolinolein were linear in the range of 0.20-50 mg/L, 0.20-50 mg/L, 0.25-62.5 mg/L and 0.20-50 mg/L, respectively, with correlation coefficients not less than 0.9983. The limits of quantification (S/N≥10) of the four fatty acid monoglycerides were 0.018-0.046 mg/L. The average recoveries for the four monoglycerides at three spiked levels were 88.0%-110.5% with relative standard deviations of 1.1%-4.1%. The proposed method showed high performance, good selectivity and fast analysis for the underivatized monoglyceride samples. It would provide a new chromatographic technology for the content analysis of monoglycerides in the emulsifier.
- Ultra-performance convergence chromatography-mass spectrometry,
- Fatty acid monoglyceride,
- Emulsifier,
- Underivatization
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