Citation: ZHU Jing, LI Yan, YONG Li, ZHENG Bo, ZOU Xiao-Li, LIU Zhen. Determination of Metabolites of Benzene Compounds and Trichloroethylene in Urinary by Isotope Dilution-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(1): 81-87. doi: 10.11895/j.issn.0253-3820.150497 shu

Determination of Metabolites of Benzene Compounds and Trichloroethylene in Urinary by Isotope Dilution-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

ZHU Jing ¹ ,
LI Yan ¹ ,
YONG Li ² ,
ZHENG Bo ¹ ,
ZOU Xiao-Li ^1,, ,
LIU Zhen ³

1.
¹ Department of Laboratory Technology and Science of Public Health, West China School of Public Health, Sichuan University, Chengdu 610041, China;
2.
² Center for Disease Control and Prevention of Sichuan Province, Chengdu 610041, China;
3.
³ National Center for Birth Defect Monitoring, West China Second University Hospital, Sichuan University, Chengdu 610041, China

Corresponding author: ZOU Xiao-Li,
Received Date: 17 June 2015
Available Online: 21 October 2015
Fund Project: 本文系国家重点基础研究发展计划(No.2010CB529502)资助 (No.2010CB529502)

A method for simultaneous determination of metabolites of benzene compounds and trichloroethylene in pregnant urine by isotope dilution-ultra performance liquid chromatography-tandem mass spectrometry was established. Urine samples were diluted 10 times with ammonium acetate buffer containing working mixed internal standard and the filtrate was injected into UPLC/MS/MS for analysis. UPLC-MS/MS was performed on Waters C₁₈ column and the gradient elution was used for separation. The negative electrospray ionization (ESI) source under the multiple reaction monitoring (MRM) mode was used for quantitative analysis. The limits of detection (LOD) ranged from 0.0350 μg/L to 1.75 μg/L. The inter-day and intra-day precisions of method were 1.2%-9.1% and 2.0%-9.7%, respectively. The proposed method was applied on detecting 650 urine samples of pregnant women in different region and the results were analyzed by statistical method. The spiked recoveries were in the range of 85.0%-104%. The method was quick, sensitive and suitable for batch analysis of urine samples.
- Isotope dilution,
- Ultra performance liquid chromatography-tandem mass spectrometry,
- Benzene compounds,
- Trichloroethylene,
- Metabolite,
- Urinary
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