Citation: ZHANG Jing-Jing, KANG Tian-Fang, LU Li-Ping, CHENG Shui-Yuan. Electrochemical Immunosensor for Microcystins Based on Magnetic Ferriferrous Oxide Nanoparticles Modified Electrode[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(5): 760-766. doi: 10.11895/j.issn.0253-3820.141067 shu

Electrochemical Immunosensor for Microcystins Based on Magnetic Ferriferrous Oxide Nanoparticles Modified Electrode

Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China

Received Date: 8 April 2015
Revised Date: 17 December 2015

Fund Project: This work was supported by the Doctoral Fund of China Education Ministry (No. 20131103110011), the China Postdoctoral Science Foundation (No.71005011201501), and the key program of Beijing Natural Science Foundation (No. KZ201110005006)

An immunosensor for detection of microcystin-(leucine-arginine) (MCLR) was constructed. Chemical co-precipitation method was used to synthesize ferriferrous oxide magnetic nanoparticles (Fe₃O₄ MNPs). The Fe₃O₄ MNPs were modified with 3-aminopropyltriethoxysilane (APTS) and succinic anhydride (SAH) in turn to generate carboxyl-functionalized core-shell MNPs (Fe₃O₄@APTS·SAH MNPs), which were characterized by transmission electron microscope (TEM), hysteresis loop, X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR), respectively. The Fe₃O₄@APTS·SAH MNPs were modified onto the surface of a home-made magnetic glassy carbon electrode (MGCE). Anti-MCLR antibody (anti-MCLR) was immobilized on the modified MGCE by coupling reaction with ethyl-3-(dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). Subsequently, bovine serum albumin (BSA) was used to block the non-specific adsorption sites of the electrode. Direct competitive immunoassay format was adopted. In the presence of horseradish peroxidase-conjugated MCLR (MCLR-HRP), MCLR in solution was determined by differential pulse voltammetry (DPV). Under the optimized conditions, MCLR could be detected within a wide linear range of 0.05-100 μg/L with a detection limit of 0.01 μg/L (S/N=3). Moreover, the immunosnssoe exhibited good reproducibility, stability and selectivity. The immunosensor was applied for the determination of MCLR in real water samples with the recoveries from 94.3% to 99.5%.
- Magnetic nanoparticles,
- Microcystin,
- Immunosensor,
- Differential pulse voltammetry
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