Citation: Yingpeng ZHANG, Xingxing LI, Yunshang YANG, Zhidong TENG. A pyrazole-based turn-off fluorescent probe for visual detection of hydrazine[J]. Chinese Journal of Inorganic Chemistry, ;2025, 41(7): 1301-1308. doi: 10.11862/CJIC.20250064 shu

A pyrazole-based turn-off fluorescent probe for visual detection of hydrazine

1.
School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China
2.
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Corresponding author: Yingpeng ZHANG, yingpengzhang@126.com
Received Date: 25 February 2025
Revised Date: 24 April 2025

Figures(14)

A fluorescence quenching probe (THI) for visually detecting hydrazine (N₂H₄) was synthesized and characterized by Knoevenagel condensation reaction using pyrazole derivatives as fluorescent groups. Spectroscopic studies revealed that, under optimal excitation and emission wavelengths, the THI exhibited high selectivity and sensitivity toward N₂H₄, maintaining excellent anti-interference performance even in the presence of various analytes. Additionally, the THI demonstrated ultrafast response time (18 s), attributed to the efficient nucleophilic addition reaction between its optimized molecular recognition site and N₂H₄. The detection limit for N₂H₄ was determined to be 0.141 μmol·L^-1, meeting the requirements for trace-level analysis. The detection mechanism, verified by mass spectrometry and density functional theory calculations, is ascribed to N₂H₄-induced intramolecular charge transfer (ICT), leading to fluorescence quenching. Furthermore, THI was successfully fabricated into test strips for the quantitative detection of N₂H₄ and applied for fluorescence imaging of N₂H₄ in HeLa cells.
- hydrazine,
- pyrazole derivative,
- intramolecular charge transfer,
- visual detection,
- fluorescent probe
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沈阳化工大学材料科学与工程学院沈阳 110142