Citation: Xiaowei TANG, Shiquan XIAO, Jingwen SUN, Yu ZHU, Xiaoting CHEN, Haiyan ZHANG. A zinc complex for the detection of anthrax biomarker[J]. Chinese Journal of Inorganic Chemistry, ;2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173 shu

A zinc complex for the detection of anthrax biomarker

1.
Office of Academic Research, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China
2.
School of Pharmacy, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China

Corresponding author: Haiyan ZHANG, 13766559005@163.com
Received Date: 14 May 2024
Revised Date: 26 June 2024

Figures(12)

A novel metal coordination polymer {[Zn₂(L)(H₂O)(DMA)] ·DMA·2.3H₂O}_n (1) has been isolated under solvothermal method, where L^4- is the four-deprotonated N, N'-bis(4-carboxybenzyl)-5-aminoisophthalic acid and DMA is N, N-dimethylacetamide. Single crystal X-ray diffraction results showed that the complex belongs to the triclinic crystal system with the space group of P1, a=0.989 6(5) nm, b=1.370 5(5) nm, c=1.382 1(5) nm, α = 80.067(5)°, β =76.729(5)°, γ =76.611(5)° and the structure of complex 1 is a 3D supramolecular framework that expanded by 2D metal-organic layers through π…π interactions. Interestingly, a"bowl"shaped structure exists in the 2D layer, and the center of the"bowl"is a cavity with a size of about 1.493 nm×1.503 nm, which can be used to load a certain volume of guest molecules. Infrared spectroscopy verified the successful coordination of zinc ions with the L^4- ligand. Powder X-ray diffraction (PXRD) experiments confirmed that complex 1 had high purity. Thermo-gravimetric analysis showed that complex 1 had good thermal stability in a range from room temperature to 416.9 ℃. Under the excitation light of 273 nm, complex 1 exhibited strong fluorescence emission at 437 nm in an ethanol solution, which could quickly detect the anthrax biomarker of pyridine-2, 6-dicarboxylic acid (DPA) through fluorescence quenching within 30 s. The results of concentration-dependent experiments showed that there was a good linear relationship between the concentration of DPA and the fluorescence intensity of complex 1, with R²=0.996 51 and a quenching constant value of 4.052×10⁴ L·mol^-1. The calculated limit of detection was approximately 15 μmol·L^-1, which was significantly lower than the infection dose of anthrax spores (60 μmol·L^-1). After adding various inter-ferents to the solution of complex 1, the change in fluorescence intensity of complex 1 was negligible. However, when DPA was added, the fluorescence intensity decreased significantly, indicating that complex 1 had a high selectivity for DPA. The detection mechanism was revealed by combining PXRD patterns and UV-Vis absorption spectra, which is fluorescence quenching induced by the collapse of the crystal framework.
- coordination polymer,
- solvothermal synthesis,
- crystal structure,
- anthrax,
- pyridine-2, 6-dicarboxylic acid,
- biomakers,
- fluorescence sensing
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