Citation: Yu-Yun ZHENG, Jing-Hong LIU, Jia-Yun CHEN, Jian-Ming OUYANG. Effects of supersaturation, Ca²⁺/C₂O₄^2- stoichiometric ratio on calcium oxalate crystallization and the regulation of degraded Poria cocos polysaccharide[J]. Chinese Journal of Inorganic Chemistry, ;2023, 39(5): 794-804. doi: 10.11862/CJIC.2023.058 shu

Effects of supersaturation, Ca²⁺/C₂O₄^2- stoichiometric ratio on calcium oxalate crystallization and the regulation of degraded Poria cocos polysaccharide

College of Chemistry and Materials Science, Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China

Corresponding author: Jian-Ming OUYANG, toyjm@jnu.edu.cn
Received Date: 19 July 2022
Revised Date: 15 March 2023

Figures(7)

To study the nucleation, growth, and aggregation of calcium oxalate (CaC₂O₄) crystals, and to explore the inhibitory effect of degraded Poria cocos polysaccharide (PCP), CaC₂O₄ crystals formed under different conditions were characterized by X-ray diffraction, FT-IR, scanning electron microscope, Raman spectroscopy, ζ potentiometer, and UV spectrophotometer. The results showed that calcium oxalate monohydrate (COM) crystals were mainly formed at low supersaturation (RS ≤ 26.6). At RS being 37.6 and 46.0, 11.6% and 38.3% calcium oxalate dihydrate (COD) crystals were formed respectively, and the aggregation degree of the crystals increased at high RS. At the same RS, the proportion of COD in the crystal increased with the increase of the Ca²⁺/Ox^2- stoichiometric ratio (n_Ca²⁺/n_Ca^2-, Ox^2-=C₂O₄^2-). The addition of degraded PCP could increase the concentration of soluble Ca²⁺ ions in the system, reduce the quality of generated CaC₂O₄ crystals, and increase the absolute value of ζ potential on the crystal surface, all of which are beneficial to inhibit the formation of CaC₂O₄ stones. Thus, the risk of high Ox^2- concentration on the formation of kidney stones is much greater than that of high Ca²⁺ concentration, suggesting that the risk of oxalic acid intake on CaC₂O₄ stones is much greater than that of calcium intake. PCP can simultaneously inhibit the nucleation, growth, and aggregation of CaC₂O₄ crystals.
- calcium oxalate,
- Poria cocos polysaccharide,
- Ca²⁺/Ox^2- stoichiometric ratio,
- supersaturation,
- crystal regulation
1. [1]
  Šafranko S, Goman S, Goman D, Jokić S, Marion I D, Mlinarić N M, Selmani A, Medvidović-Kosanović M, Stanković A. Calcium oxalate and gallic acid: Structural characterization and process optimization toward obtaining high contents of calcium oxalate monohydrate and dihydrate[J]. Crystals, 2021,11(8)954. doi: 10.3390/cryst11080954
2. [2]
  CHENG X Y, XU M, OUYANG J M. Synthesis, characterization, adsorption properties and toxicity to renal epithelial cells of calcium oxalate monohydrate crystals with different aspect ratios[J]. Chinese J. Inorg. Chem., 2022,38(7):1261-1271.
3. [3]
  Wang Z, Zhang Y, Zhang J W, Deng Q, Liang H. Recent advances on the mechanisms of kidney stone formation[J]. Int. J. Mol. Med., 2021,48(2)149. doi: 10.3892/ijmm.2021.4982
4. [4]
  Sun X Y, Zhang H, Deng J W, Yu B X, Zhang Y H, Ouyang J M. Regulatory effects of damaged renal epithelial cells after repair by Porphyra yezoensis polysaccharides with different sulfation degree on the calcium oxalate crystal-cell interaction[J]. Int. J. Nanomed., 2021,16:8087-8102. doi: 10.2147/IJN.S320278
5. [5]
  Wang N, Zhang D, Zhang Y T, Xu W, Wang Y S, Zhong P P, Jia T Z, Xiu Y F. Endothelium corneum gigeriae Galli extract inhibits calcium oxalate formation and exerts anti-urolithic effects[J]. J. Ethnopharmacol., 2019,231:80-89. doi: 10.1016/j.jep.2018.09.003
6. [6]
  Singh A, Tandon S, Nandi S P, Kaur T, Tandon C. Downregulation of inflammatory mediators by ethanolic extract of Bergenia ligulata (Wall.) in oxalate injured renal epithelial cells[J]. J. Ethnopharmacology, 2021,275114104. doi: 10.1016/j.jep.2021.114104
7. [7]
  Chen X W, Sun X Y, Tang G H, Ouyang J M. Sulfated Undaria pinnatifida polysaccharide inhibits the formation of kidney stones by inhibiting HK-2 cell damage and reducing the adhesion of nano-calcium oxalate crystals[J]. Biomaterials Advances, 2021,134112564.
8. [8]
  Akın B, Öner M, Bayram Y, Demadis K D. Effects of carboxylate-modified, "green" inulin biopolymers on the crystal growth of calcium oxalate[J]. Cryst. Growth Des., 2008,8(6):1997-2005. doi: 10.1021/cg800092q
9. [9]
  Zhao Y W, Guo D, Li C Y, Ouyang J M. Comparison of the adhesion of calcium oxalate monohydrate to HK-2 cells before and after repair using tea polysaccharides[J]. Int. J. Nanomed., 2019,14:4277-4292. doi: 10.2147/IJN.S198644
10. [10]
  Sun Y. Biological activities and potential health benefits of polysaccharides from Poria cocos and their derivatives[J]. Int. J. Biol. Macromol., 2014,68:131-134. doi: 10.1016/j.ijbiomac.2014.04.010
11. [11]
  Chen F, Huang G L. Preparation and immunological activity of polysaccharides and their derivatives[J]. Int. J. Biol. Macromol., 2018,112:211-216. doi: 10.1016/j.ijbiomac.2018.01.169
12. [12]
  Cheung Y C, Yin J, Wu J Y. Effect of polysaccharide chain conformation on ultrasonic degradation of curdlan in alkaline solution[J]. Carbohydr. Polym., 2018,195:298-302. doi: 10.1016/j.carbpol.2018.04.118
13. [13]
  Jin Y, Zhang L, Tao Y, Zeng C, Chen Y, Cheung P C K. Solution properties of a water-insoluble (1→3)-α-D-glucan isolated from Poria cocos mycelia[J]. Carbohydr. Polym., 2004,57(2):205-209. doi: 10.1016/j.carbpol.2004.04.013
14. [14]
  Zhang Y Q, Tang Y M, Xu J Q, Zhang D Q, Lu G, Jing W H. Modulation of polyepoxysuccinic acid on crystallization of calcium oxalate[J]. Solid State Chem., 2015,231:7-12. doi: 10.1016/j.jssc.2015.08.001
15. [15]
  Stanković A, Kontrec J, Džakula B N, Dzakula B N, Kovacevic D, Markovic B, Kralj D. Preparation and characterization of calcium oxalate dihydrate seeds suitable for crystal growth kinetic analyses[J]. J. Cryst. Growth, 2018,500:91-97. doi: 10.1016/j.jcrysgro.2018.08.020
16. [16]
  Donnet M, Jongen N, Lemaitre J, Bowen P. New morphology of calcium oxalate trihydrate precipitated in a segmented flow tubular reactor[J]. Mater. Sci. Lett., 2000,19:749-750. doi: 10.1023/A:1006771428827
17. [17]
  Chen X W, Huang W B, Sun X Y, Xiong P, Ouyang J M. Antioxidant activity of sulfated Porphyra yezoensis polysaccharides and their regulating effect on calcium oxalate crystal growth[J]. Mater. Sci. Eng. C, 2021,128112338. doi: 10.1016/j.msec.2021.112338
18. [18]
  Zhang C Y, Wu W H, Wang J, Lan M B. Antioxidant properties of polysaccharide from the brown seaweed Sargassum graminifolium (Turn.), and its effects on calcium oxalate crystallization[J]. Mar. Drugs, 2012,10(1):119-130.
19. [19]
  Bandarapalle K, Rajasekhar K K, Bhavitha J. Antiurolithiatic activity of Bacopa monnieri by in vitro calcium oxalate crystallization methods[J]. Basic Pharmacol. Toxicol., 2021,5(1):14-17.
20. [20]
  Sun X Y, Zhang C Y, Bhadja P, Ouyang J M. Preparation, properties, formation mechanisms, and cytotoxicity of calcium oxalate monohydrate with various morphologies[J]. CrystEngComm, 2018,20:75-87. doi: 10.1039/C7CE01912B
21. [21]
  Tonannavar J, Deshpande G, Yenagi J, Patil S B, Patil N, Mulimani B G. Identification of mineral compositions in some renal calculi by FT Raman and IR spectral analysis[J]. Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 2016,154:20-26. doi: 10.1016/j.saa.2015.10.003
22. [22]
  Kazemi-Zanjani N, Chen H, Goldberg H A, Hunter G, Grohe B, Lagugne-Labarthet F. Label-free mapping of osteopontin adsorption to calcium oxalate monohydrate crystals by tip-enhanced Raman spectroscopy[J]. J. Am. Chem. Soc., 2012,134(41):17076-17082. doi: 10.1021/ja3057562
23. [23]
  Muhammed Shameem K M, Chawla A, Mallya M, Unnikrishnan B K B V K, Santhosh V B K. Laser-induced breakdown spectroscopy Raman: An effective complementary approach to analyze renal calculi[J]. J. Biophotonics, 2018,11(6)e201700271. doi: 10.1002/jbio.201700271
24. [24]
  Kulaksızoğlu S, Sofikerim M, Çevik C. In vitro effect of lemon and orange juices on calcium oxalate crystallization[J]. Int. Urol. Nephrol., 2008,40(3):589-594. doi: 10.1007/s11255-007-9256-0
25. [25]
  Daudon M, Letavernier E, Frochot V, Haymann J, Bazin D, Jungers P. Respective influence of calcium and oxalate urine concentration on the formation of calcium oxalate monohydrate or dihydrate crystals[J]. C. R. Chim., 2016,19(11/12):1504-1513.
26. [26]
  Gan Q Z, Sun X Y, Bhadja P, Yao X Q, Ouyang J M. Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: Aggravation of crystal adhesion and aggregation[J]. Int. J. Nanomed., 2016,11:2839-2854.
27. [27]
  Rodgers A. Aspects of calcium oxalate crystallization: Theory, in vitro studies, and in vivo implementation[J]. J. Am. Soc. Nephrol., 1999,10:S351-S354.
28. [28]
  Sorensen, Kahn, Reiner, Tseng, Shikany, Wallace, Chi, Wactawski-Wende, Jackson, O'Sullivan, Sadetsky, Stoller, WHI Working Group. Impact of nutritional factors on incident kidney stone formation: A report from the WHI OS[J]. J. Urol., 2012,187(5):1645-1650. doi: 10.1016/j.juro.2011.12.077
29. [29]
  Liu H, Sun X Y, Wang F X, Ouyang J M. Regulation on calcium oxalate crystallization and protection on HK-2 cells of tea polysaccharides with different molecular weights[J]. Oxidative Med. Cell. Longev., 20205057123.
30. [30]
  Grohe B, Rogers K A, Goldberg H A, Hunter G K. Crystallization kinetics of calcium oxalate hydrates studied by scanning confocal interference microscopy[J]. J. Cryst. Growth, 2006,295(2):148-157. doi: 10.1016/j.jcrysgro.2006.07.029
31. [31]
  Farmanesh S, Ramamoorthy S, Chung J, Asplin J R A, Karande P, Rimer J D. Specificity of growth inhibitors and their cooperative effects in calcium oxalate monohydrate crystallization[J]. J. Am. Chem. Soc., 2014,136(1):367-376. doi: 10.1021/ja410623q
32. [32]
  Chung J, Taylor M G, Granja I, Asplin J R, Mpourmpakis G, Rimer J D. Factors differentiating the effectiveness of polyprotic acids as inhibitors of calcium oxalate crystallization in kidney stone disease[J]. Cryst. Growth Des., 2018,18(9):5617-5627. doi: 10.1021/acs.cgd.8b00945
33. [33]
  Abdel-Aal E A, Yassin A M K, El-Shahat M F. Effect of crystallization parameters and presence of aqueous extract of Nigella Sativa on growth inhibition of calcium oxalate monohydrate particles[J]. Part. Sci. Technol., 2018,36(2):226-234. doi: 10.1080/02726351.2016.1241847
34. [34]
  Polat S, Eral H B. Elucidating the role of hyaluronic acid in the structure and morphology of calcium oxalate crystals[J]. Adv. Powder Technol., 2021,32(10):3650-3659. doi: 10.1016/j.apt.2021.08.021
1. [1]
  Xinyu ZENG , Guhua TANG , Jianming OUYANG . Inhibitory effect of Desmodium styracifolium polysaccharides with different content of carboxyl groups on the growth, aggregation and cell adhesion of calcium oxalate crystals. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1563-1576. doi: 10.11862/CJIC.20230374
2. [2]
  Jiaxuan Zuo , Kun Zhang , Jing Wang , Xifei Li . 锂离子电池Ni-Co-Mn基正极材料前驱体的形核调控及机制. Acta Physico-Chimica Sinica, 2025, 41(1): 2404042-. doi: 10.3866/PKU.WHXB202404042
3. [3]
  Zijuan LI , Xuan LÜ , Jiaojiao CHEN , Haiyang ZHAO , Shuo SUN , Zhiwu ZHANG , Jianlong ZHANG , Yanling MA , Jie LI , Zixian FENG , Jiahui LIU . Synthesis of visual fluorescence emission CdSe nanocrystals based on ligand regulation. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 308-320. doi: 10.11862/CJIC.20240138
4. [4]
  Ningning Gao , Yue Zhang , Zhenhao Yang , Lijing Xu , Kongyin Zhao , Qingping Xin , Junkui Gao , Junjun Shi , Jin Zhong , Huiguo Wang . Ba²⁺/Ca²⁺ co-crosslinked alginate hydrogel filtration membrane with high strength, high flux and stability for dye/salt separation. Chinese Chemical Letters, 2024, 35(5): 108820-. doi: 10.1016/j.cclet.2023.108820
5. [5]
  Fei Xie , Chengcheng Yuan , Haiyan Tan , Alireza Z. Moshfegh , Bicheng Zhu , Jiaguo Yu . d带中心调控过渡金属单原子负载COF吸附O₂的理论计算研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2407013-. doi: 10.3866/PKU.WHXB202407013
6. [6]
  Jingwen Wang , Minghao Wu , Xing Zuo , Yaofeng Yuan , Yahao Wang , Xiaoshun Zhou , Jianfeng Yan . Advances in the Application of Electrochemical Regulation in Investigating the Electron Transport Properties of Single-Molecule Junctions. University Chemistry, 2025, 40(3): 291-301. doi: 10.12461/PKU.DXHX202406023
7. [7]
  Yan Liu , Yuexiang Zhu , Luhua Lai . Introduction to Blended and Small-Class Teaching in Structural Chemistry: Exploring the Structure and Properties of Crystals. University Chemistry, 2024, 39(3): 1-4. doi: 10.3866/PKU.DXHX202306084
8. [8]
  Zhaoyang WANG , Chun YANG , Yaoyao Song , Na HAN , Xiaomeng LIU , Qinglun WANG . Lanthanide(Ⅲ) complexes derived from 4′-(2-pyridyl)-2, 2′∶6′, 2″-terpyridine: Crystal structures, fluorescent and magnetic properties. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1442-1451. doi: 10.11862/CJIC.20240114
9. [9]
  Fan JIA , Wenbao XU , Fangbin LIU , Haihua ZHANG , Hongbing FU . Synthesis and electroluminescence properties of Mn²⁺ doped quasi-two-dimensional perovskites (PEA)₂Pb_yMn_1-yBr₄. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1114-1122. doi: 10.11862/CJIC.20230473
10. [10]
  Liyang ZHANG , Dongdong YANG , Ning LI , Yuanyu YANG , Qi MA . Crystal structures, luminescent properties and Hirshfeld surface analyses of three cadmium(Ⅱ) complexes based on 2-(3-(pyridin-2-yl)-1H-pyrazol-1-yl)benzoate. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1943-1952. doi: 10.11862/CJIC.20240079
11. [11]
  Jiarui Wu , Gengxin Wu , Yan Wang , Yingwei Yang . Crystal Engineering Based on Leaning Towerarenes. University Chemistry, 2024, 39(3): 58-62. doi: 10.3866/PKU.DXHX202304014
12. [12]
  Zeyuan WANG , Songzhi ZHENG , Hao LI , Jingbo WENG , Wei WANG , Yang WANG , Weihai SUN . Effect of I₂ interface modification engineering on the performance of all-inorganic CsPbBr₃ perovskite solar cells. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1290-1300. doi: 10.11862/CJIC.20240021
13. [13]
  Xiaoyao YIN , Wenhao ZHU , Puyao SHI , Zongsheng LI , Yichao WANG , Nengmin ZHU , Yang WANG , Weihai SUN . Fabrication of all-inorganic CsPbBr₃ perovskite solar cells with SnCl₂ interface modification. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 469-479. doi: 10.11862/CJIC.20240309
14. [14]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113
15. [15]
  Cheng PENG , Jianwei WEI , Yating CHEN , Nan HU , Hui ZENG . First principles investigation about interference effects of electronic and optical properties of inorganic and lead-free perovskite Cs₃Bi₂X₉ (X=Cl, Br, I). Chinese Journal of Inorganic Chemistry, 2024, 40(3): 555-560. doi: 10.11862/CJIC.20230282
16. [16]
  Lijuan Liu , Xionglei Wang . Preparation of Hydrogels from Waste Thermosetting Unsaturated Polyester Resin by Controllable Catalytic Degradation: A Comprehensive Chemical Experiment. University Chemistry, 2024, 39(11): 313-318. doi: 10.12461/PKU.DXHX202403060
17. [17]
  Haiyu Nie , Chenhui Zhang , Fengpei Du . Ideological and Political Design for the Preparation, Characterization and Particle Size Control Experiment of Nanoemulsion. University Chemistry, 2024, 39(2): 41-46. doi: 10.3866/PKU.DXHX202306055
18. [18]
  Pei Li , Yuenan Zheng , Zhankai Liu , An-Hui Lu . Boron-Containing MFI Zeolite: Microstructure Control and Its Performance of Propane Oxidative Dehydrogenation. Acta Physico-Chimica Sinica, 2025, 41(4): 100034-. doi: 10.3866/PKU.WHXB202406012
19. [19]
  Dongheng WANG , Si LI , Shuangquan ZANG . Construction of chiral alkynyl silver chains and modulation of chiral optical properties. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 131-140. doi: 10.11862/CJIC.20240379
20. [20]
  Qingjun PAN , Zhongliang GONG , Yuwu ZHONG . Advances in modulation of the excited states of photofunctional iron complexes. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 45-58. doi: 10.11862/CJIC.20240365

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(1466)
HTML views(180)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Effects of supersaturation, Ca2+/C2O42- stoichiometric ratio on calcium oxalate crystallization and the regulation of degraded Poria cocos polysaccharide

Metrics

通讯作者: 陈斌, bchen63@163.com

Effects of supersaturation, Ca²⁺/C₂O₄^2- stoichiometric ratio on calcium oxalate crystallization and the regulation of degraded Poria cocos polysaccharide