Citation: Xiao-Yan CHENG, Meng XU, Jian-Ming OUYANG. Synthesis, Characterization, Adsorption Properties of Calcium Oxalate Monohydrate Crystals with Different Aspect Ratios and Their Toxicity to Renal Epithelial Cell[J]. Chinese Journal of Inorganic Chemistry, ;2022, 38(7): 1261-1271. doi: 10.11862/CJIC.2022.135 shu

Synthesis, Characterization, Adsorption Properties of Calcium Oxalate Monohydrate Crystals with Different Aspect Ratios and Their Toxicity to Renal Epithelial Cell

Institute of Biomineralization and Lithiasis Research, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China

Corresponding author: Jian-Ming OUYANG, toyjm@jnu.edu.cn
Received Date: 7 December 2021
Revised Date: 20 April 2022

Figures(8)

Four kinds of calcium oxalate monohydrate (COM) crystals (COM-1:2, COM-1:3, COM-1:4, and COM-1:5) with the aspect ratio of 1:2, 1:3, 1:4, and 1:5, respectively, were synthesized. Their physicochemical properties were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscope (SEM), ζ potentiometer, and specific surface tester. SEM images show that the widths of the crystals were similar, but the lengths of the crystals were (3±0.3) μm, (5.2±0.3) μm, (7.0±0.7) μm, and (8.8±1.2) μm, respectively. With the increase of reaction temperature, the length of the crystal increased. The faster the stirring speed, the smaller the crystal size. As the concentration of the additive gelatin decreased, the (101) faces of COM crystals were elongated. Cell viability, cell total mortality, and reactive oxygen species (ROS) tests showed that the toxicity of COM with different length-width ratios to the human proximal tubular epithelial cells (HK-2) was COM-1:2 > COM-1:3 > COM-1:4 > COM-1:5 > control group. SEM examination showed that all four COM crystals could adhere to the cell surface. The reasons for this difference are positively related to the following points: a large proportion of (101) crystal planes, large specific surface area, and small cell-crystal shear stress.
- calcium oxalate monohydrate,
- size effect,
- cell damage,
- kidney stones,
- crystal plane
1. [1]
  Khan S R, Pearle M S, Robertson W G, Gambaro G, Canales B K, Doizi S, Traxer O, Tiselius H G. Kidney Stones[J]. Nat. Rev. Dis. Primers, 2016,2(1)16008. doi: 10.1038/nrdp.2016.8
2. [2]
  Khan A H, Imran S, Talati J, Jafri L. Fourier Transform Infrared Spectroscopy for Analysis of Kidney Stones[J]. Invest. Clin. Urol., 2018,59(1):32-37. doi: 10.4111/icu.2018.59.1.32
3. [3]
  Sun X Y, Zhang H, Chen J Y, Zeng G H, Ouyang J M. Porphyra Yezoensis Polysaccharide and Potassium Citrate Synergistically Inhibit Calcium Oxalate Crystallization Induced by Renal Epithelial Cells and Cytotoxicity of the Formed Crystals[J]. Sci. Eng. C, 2021,119111448. doi: 10.1016/j.msec.2020.111448
4. [4]
  Liu H R, Ye T, Yang X Q, Liu J H, Jiang K H, Lu H Y, Xia D, Peng E, Chen Z Q, Sun F, Tang K, Ye Z Q. H19 Promote Calcium Oxalate Nephrocalcinosis-Induced Renal Tubular Epithelial Cell Injury via a ceRNA Pathway[J]. EBioMedicine, 2019,50:366-378. doi: 10.1016/j.ebiom.2019.10.059
5. [5]
  WEN X L, SUN X Y, OUYANG J M. Adsorption Properties of Positively Charged Proteins on Nano/Micron Calcium Oxalate Crystals and Their Comparison with Negatively Charged Proteins[J]. Chinese J. Inorg. Chem., 2017,33(1):49-56. doi: 10.11862/CJIC.2017.013
6. [6]
  Ouyang J M, Xia Z Y, Zhang G N, Chen H Q. Nanocrystallites in Urine and Their Relationship with the Formation of Kidney Stones[J]. Rev. Inorg. Chem., 2012,32(2/3/4):101-110.
7. [7]
  Lou Y T, He W, Song Z Y. Aggregation of Nanochemical Microcrystals in Urine Promotes the Formation of Urinary Calculi[J]. J. Chem., 20208516903.
8. [8]
  Jo M, Oh Y, Kim H J, Kim H L, Yang S H. Diffusion-Controlled Crystallization of Calcium Carbonate in a Hydrogel[J]. Cryst. Growth Des., 2020,20(2):560-567. doi: 10.1021/acs.cgd.9b00614
9. [9]
  Chen X S, Jiang R L, Zhou Z H, Wang X W. Synthesis and Catalytic Properties of ZSM-5 Crystals with Different Morphologies in Gelatin Hydrogels[J]. J. Disper. Sci. Technol., 2019,42(4):561-568.
10. [10]
  An D C, Wang J J, Zhang J, Zhai X, Kang Z P, Fan W H, Yan J, Liu Y Q, Lu L, Jia C L, Wuttig M, Cojocaru-Mirédin O, Chen S P, Wang W X, Snyder G J, Yu Y. Retarding Ostwald Ripening through Gibbs Adsorption and Interfacial Complexions Leads to High-Performance SnTe Thermoelectrics[J]. Energy Environ. Sci., 2021,14(10):5469-5479. doi: 10.1039/D1EE01977E
11. [11]
  Pitto-Barry A, Barry N P E. Effect of Temperature on the Nucleation and Growth of Precious Metal Nanocrystals[J]. Angew. Chem. Int. Ed., 2019,58(51):18482-18486. doi: 10.1002/anie.201912219
12. [12]
  Zhang X, Wang Y, Liu D R, Ji Z S, Xu H Y, Hu M L, Cui P X. Effect of Stirring Rate on Grain Morphology of Mg-Al Alloy Semi-Solid Structure by Phase Field Lattice Boltzmann Simulation[J]. J. Cryst. Growth., 2020,543125704. doi: 10.1016/j.jcrysgro.2020.125704
13. [13]
  CAO J, CAI Y R, MA Y S, YAO J M. Effects of Nano-Calcium Phosphate on Biological Behavior of MG63 Cells[J]. Chinese Journal of Tissue Engineering Research, 2012,16(29):5341-5344.
14. [14]
  Tamura K, Takashi N, Kumazawa R, Watari F, Totsuka Y. Effects of Particle Size on Cell Function and Morphology in Titanium and Nickel[J]. Mater. Trans., 2002,43(12):3052-3057. doi: 10.2320/matertrans.43.3052
15. [15]
  Sheng X X, Ward M D, Wesson J A. Crystal Surface Adhesion Explains the Pathological Activity of Calcium Oxalate Hydrates in Kidney Stone Formation[J]. J. Am. Soc. Nephrol., 2005,16(7):1904-1908. doi: 10.1681/ASN.2005040400
16. [16]
  Farmanesh S, Ramamoorthy S, Chung J, Asplin J R, Karande P, Rimer J D. Specificity of Growth Inhibitors and Their Cooperative Effects in Calcium Oxalate Monohydrate Crystallization[J]. J. Am. Chem. Soc., 2014,136(1):367-376. doi: 10.1021/ja410623q
17. [17]
  King M, Mcclure W F, Andrews L C. Powder Diffraction File Alpha-betical Index, Inorganic Phases/Organic Phases. International Center for Diffraction Data, U.S.A., 1992.
18. [18]
  Wang L, He X M, Sun W T, Wang J L, Li Y D, Fan S S. Crystal Orientation Tuning of LiFePO₄ Nanoplates for High Rate Lithium Battery Cathode Materials[J]. Nano Lett., 2012,12(11):5632-5636. doi: 10.1021/nl3027839
19. [19]
  Jiang K H, Hu J X, Luo G H, Song D L, Zhang P, Zhu J G, Sun F. MiR-155-5p Promotes Oxalate- and Calcium-Induced Kidney Oxidative Stress Injury by Suppressing MGP Expression[J]. Oxid. Med. Cell. Longevity, 20205863617.
20. [20]
  Zhang L M, Zhen R R, Gu C, Zhang T L, Li Y, Jin M, Hu B, An H M. Chinese Medicine Di-Huang-Yi-Zhi Protects PC12 Cells from H₂O₂-Induced Apoptosis by Regulating ROS-ASK1-JNK/p38 MAPK Signaling[J]. BMC Complement. Med. Ther., 2020,20(1)54. doi: 10.1186/s12906-020-2834-8
21. [21]
  Sun X Y, Yu K, Ouyang J M. Time-Dependent Subcellular Structure Injuries Induced by Nano -/Micron-Sized Calcium Oxalate Monohydrate and Dihydrate Crystals[J]. Mater. Sci. Eng. C, 2017,79:445-456. doi: 10.1016/j.msec.2017.05.081
22. [22]
  Sun X Y, Gan Q Z, Ouyang J M. Size-Dependent Cellular Uptake Mechanism and Cytotoxicity toward Calcium Oxalate on Vero Cells[J]. Sci. Rep., 2017,741949. doi: 10.1038/srep41949
23. [23]
  Narula S, Tandon S, Singh S K, Tandon C. Kidney Stone Matrix Proteins Ameliorate Calcium Oxalate Monohydrate Induced Apoptotic Injury to Renal Epithelial Cells[J]. Life. Sci., 2016,164:23-30. doi: 10.1016/j.lfs.2016.08.026
24. [24]
  Sheng X X, Jung T S, Wesson J A, Ward M D. Adhesion at Calcium Oxalate Crystal Surfaces and the Effect of Urinary Constituents[J]. Proc. Natl. Acad. Sci. U.S.A., 2005,102(2):267-272. doi: 10.1073/pnas.0406835101
25. [25]
  Nel A, Xia T, Madle L, Li N. Toxic Potential of Materials at the Nanolevel[J]. Science, 2006,311(5761):622-627. doi: 10.1126/science.1114397
26. [26]
  Patil V R S, Campbell C J, Yun Y H, Slack S M, Goetz D J. Particle Diameter Influences Adhesion under Flow[J]. Biophys. J., 2001,80(4):1733-1743. doi: 10.1016/S0006-3495(01)76144-9
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