Citation: RAO Chen-Ying, GUO Da, SUN Xin-Yuan, OUYANG Jian-Ming. Toxicity Difference of Nano and Micron Calcium Oxalate Monohydrate on Renal Epithelial Cells[J]. Chinese Journal of Inorganic Chemistry, ;2019, 35(3): 467-476. doi: 10.11862/CJIC.2019.039 shu

Toxicity Difference of Nano and Micron Calcium Oxalate Monohydrate on Renal Epithelial Cells

Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China

Corresponding author: OUYANG Jian-Ming, toyjm@jnu.edu.cn
Received Date: 12 October 2018
Revised Date: 28 November 2018

Figures(7)

Scanning electron microscope(SEM), laser confocal microscope(LSM), flow cytometry methods were used to comparatively study the toxicity difference of nano and micron calcium oxalate monohydrate(COM)on the African green monkey kidney epithelial(Vero)cells. The results revealed that both nano and micron COM could decrease cell viability, increase LDH release amount, up-regulate HA expression, decrease lysosomal integrity, decrease mitochondrial membrane potential, increased cell death rate and arrest cell cycle, which indicated that both nano and micron COM crystals could damage Vero cells and presented concentration dependent. But nano COM exhibited higher cytotoxicity and more adhesion amount than micron COM. This study may provide insights into the damage to renal epithelial cells induced by urinary crystals and the formation mechanism of kidney stones.
- Calcium oxalate,
- size effect,
- crystal adhesion,
- subcellular organelle injury
1. [1]
  Purnell S, Gallante B, Blum K, et al. J. Urology, 2018, 199(4):e413-e414
2. [2]
  WU Zhi-Fu, WU Han-Kui, GAO Yan-Ping. Chem. Bull., 2014, 77(11):1113-1115
3. [3]
  YANG Hong-Hua. Contemporary Medicine, 2018(3):107-108 doi: 10.3969/j.issn.1009-4393.2018.03.050
4. [4]
  Xu L H R, Adams-Huet B, Poindexter J R, et al. J. Urology, 2017, 197(6):1465-1471 doi: 10.1016/j.juro.2017.01.057
5. [5]
  Unno R, Kawabata T, Takase H, et al. J. Urology, 2018, 199(4):e76
6. [6]
  WANG Zhu, ZHANG Jian-Wen, ZHANG Ying, et al. Journal of Modern Urology, 2018(1):52-56
7. [7]
  Ito Y, Niimi K, Unno R, et al. J. Urology, 2018, 199(4):e291-e292
8. [8]
  GAN Qiong-Zhi, SUN Xin-Yuan, YAO Xiu -Qiong, et al. Chem. J. Chinese Universities, 2016, 37(6):1050-1059
9. [9]
  Abd El-Salam M, Bastos J K, Han J J, et al. J. Med. Chem., 2018, 61(4):1609-1621
10. [10]
  GUO Da, XU Meng, SUN Xin-Yuan, et al. Chinese J. Inorg. Chem., 2018, 34(10):1883-1890 doi: 10.11862/CJIC.2018.212
11. [11]
  Manissorn J, Fong-Ngern K, Peerapen P, et al. Sci. Rep., 2017, 7(1):1798
12. [12]
  Li Y, Sun L, Jin M H, et al. Toxicol. Vitro, 2011, 25(7):1343-1352 doi: 10.1016/j.tiv.2011.05.003
13. [13]
  Hanna L K, Johanna G, Pontus C, et al. Toxicol. Lett., 2009, 188:112-118 doi: 10.1016/j.toxlet.2009.03.014
14. [14]
  Tamura K, Takashi N, Kumazawa R, et al. Mater. Trans., 2002, 43(12):3052-3057 doi: 10.2320/matertrans.43.3052
15. [15]
  Chithrani B D, Chan W C W. Nano Lett., 2007, 7(6):1542-1550 doi: 10.1021/nl070363y
16. [16]
  Gao J, Xue J F, Xu M, et al. Int. J. Nanomed., 2014, 9:4399-4409
17. [17]
  Verdesca S, Fogazzi G B, Garigali G, et al. Clin. Chem. Lab. Med., 2011, 49(3):515-520
18. [18]
  Sun X Y, Gan Q Z, Ouyang J M. Sci. Rep., 2017, 7:41949 doi: 10.1038/srep41949
19. [19]
  Sun X Y, Ouyang J M, Gan Q Z, et al. J. Biomed. Nanotechnol., 2016, 12:2001-2014 doi: 10.1166/jbn.2016.2289
20. [20]
  King M, Mcclure W F, Andrews L C. International Center for Diffraction Data. Newtown Square, PA, USA:ICDD, 1992.
21. [21]
  Newton D A, Lottes R G, Pamtlia M K, et al. Am. J. Respir. Crit. Care Med., 2017, 195:A2391
22. [22]
  Scaffidi P, Misteli T, Bianchi M E. Nature, 2002, 418(6894):191 doi: 10.1038/nature00858
23. [23]
  Schutt F, Davies S, Kopitz J, et al. Invest. Ophthalmol. Vis. Sci., 2000, 41(8):2303-2308
24. [24]
  Gottlieb E, Vander Heiden M G, Thompson C B. Mol. Cell. Biol., 2000, 20(15):5680-5689 doi: 10.1128/MCB.20.15.5680-5689.2000
25. [25]
  Orrenius S. Drug Metab. Rev., 2007, 39(2/3):443-455
26. [26]
  Bononi A, Giorgi C, Patergnani S, et al. Nature, 2017, 546(7659):549
27. [27]
  Roos W P, Frohnapfel L, Quiros S, et al. Cancer Lett., 2018, 424:119-126 doi: 10.1016/j.canlet.2018.03.025
28. [28]
  Pang Y C, Xi J Y, Xu Y, et al. Appl. Microbiol. Biotechnol., 2016, 100(14):6435-6446 doi: 10.1007/s00253-016-7452-5
29. [29]
  Riccardi C, Nicoletti I. Nat. Protoc., 2006, 1(3):1458 doi: 10.1038/nprot.2006.238
30. [30]
  Ito T, Iida-Tanaka N, Niidome T, et al. J. Control. Release, 2006, 112(3):382-388 doi: 10.1016/j.jconrel.2006.03.013
31. [31]
  Ioachim E, Charchanti A, Briasoulis E, et al. Eur. J. Cancer, 2002, 38(18):2362-2370 doi: 10.1016/S0959-8049(02)00210-1
32. [32]
  Xi Q, Ouyang J M, Pu J X, et al. Urology, 2015, 86(4):844.e1
33. [33]
  Vinaiphat A, Aluksanasuwan S, Manissorn J, et al. Proteomics, 2017, 17(15/16):1700192
34. [34]
  Tsuji H, Wang W, Sunil J, et al. World J. Urol., 2016, 34(1):89-95 doi: 10.1007/s00345-015-1563-y
35. [35]
  Mattheolabakis G, Milane L, Singh A, et al. J. Drug Target., 2015, 23(7/8):605-618
36. [36]
  Novgorodov S A, Voltin J R, Gooz M A, et al. J. Lipid Res., 2018, 59(2):312-329 doi: 10.1194/jlr.M080374
37. [37]
  Plotegher N, Duchen M R. Trends Mol. Med., 2017, 23(2):116-134 doi: 10.1016/j.molmed.2016.12.003
38. [38]
  Zhang J, Wang J, Wong Y K, et al. Cell Death Dis., 2018, 9(6):614 doi: 10.1038/s41419-018-0571-4
39. [39]
  Lammel T, Boisseaux P, Fernández-Cruz M L, et al. Part. Fibre Toxicol., 2013, 10(2):174-184
40. [40]
  Pavan C, Turci F, Tomatis M, et al. Colloids Surf. B, 2017, 157:449-455 doi: 10.1016/j.colsurfb.2017.06.012
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