Citation: WEN Xiao-Ling, SUN Xin-Yuan, OUYANG Jian-Ming. Adsorption Properties of Cationic Protein on Nano/Micron Calcium Oxalate Crystals and a Comparison with Anionic Protein Adsorption[J]. Chinese Journal of Inorganic Chemistry, ;2017, 33(1): 49-56. doi: 10.11862/CJIC.2017.013 shu

Adsorption Properties of Cationic Protein on Nano/Micron Calcium Oxalate Crystals and a Comparison with Anionic Protein Adsorption

Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China

Corresponding author: OUYANG Jian-Ming, toyjm@jnu.edu.cn
Received Date: 14 May 2016
Revised Date: 11 November 2016

Figures(6)

The adsorption difference of cationic protein lysozyme (LSZ) on calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) crystals with a size of 100 nm and 3 μm, respectively, were investigated and compared with that of anionic protein bovine serum albumin (BSA). All of the adsorption isotherms were fitted better with Langmuir model, indicating a monolayer adsorption of LSZ on nano/micron COD and COM surface. The maximum adsorption quality of LSZ follows the order:COD-100 nm > COM-100 nm > COD-3 μm > COM-3 μm. That is, the greater the specific surface area is, the smaller the crystal curvature would be, so the greater of LSZ adsorption amount was generated; and the more negative charges and the more crystal water on crystal surface also result in the greater of LSZ adsorption. The ionic strength and pH value of system can also affect LSZ adsorption. With the concentration of NaCl increases, the adsorption quantity of LSZ reduced, it indicated that Na⁺ ions and cationic LSZ form a competitive adsorption, which reducing the adsorption sites of LSZ. The maximum adsorption of LSZ appears at the isoelectric point of LSZ (pH=10.7). In pH=5~8 (physical condition), LSZ adsorption quantity increases with the increase of pH value. The results suggest that the adsorption quality of LSZ on nano/micron COM and COD crystals can be reduced by reducing the pH value or increasing the ionic strength of urine appropriately, and thus the formation of CaOx urinary stones maybe inhibited.
- calcium oxalate,
- cationic protein,
- lysozyme,
- competitive adsorption,
- ionic strength,
- pH value
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