Citation: WANG Ling, Wen WEN, LI Sha, FU Zheng-Qing, TONG Yue-Ju, WANG Juan, HAN Dong, ZHANG Ai-Ping. Photocatalytic Killing Effect of Fe₃O₄-TiO₂ Nanoparticles on Hepatoma Carcinoma Cells for Targeting Photodynamic Therapy[J]. Chinese Journal of Inorganic Chemistry, ;2016, 32(3): 393-404. doi: 10.11862/CJIC.2016.033 shu

Photocatalytic Killing Effect of Fe₃O₄-TiO₂ Nanoparticles on Hepatoma Carcinoma Cells for Targeting Photodynamic Therapy

College of Pharmacy, Shanxi Medical University, Taiyuan 030001, China

Corresponding author: ZHANG Ai-Ping, zhangap1@163.com
Received Date: 25 August 2015
Revised Date: 4 December 2015

Figures(8)

Photodynamic therapy (PDT), as a fast developing alternative of traditional therapeutics, is showing great promise in antitumor treatment. Herein, novel photosensitizer (PS) Fe₃O₄-TiO₂ magnetic nanoparticles (NPs) were strategically designed as targeting drug with higher photocatalytic killing efficiency. The photokilling effect of Fe₃O₄-TiO₂ NPs on hepatoma carcinoma cells (HepG2) in different external magnetic fields was investigated under ultraviolet light and visible light irradiation. Meanwhile, changes of cell cycle, apoptosis rate and mitochondrial membrane potential (MMP) of HepG2 cells were detected by flow cytometry (FCM). The results indicated that Fe₃O₄-TiO₂ NPs have comparable photokilling efficiency to HepG2 cells under both visible and ultraviolet light. Moreover, Fe₃O₄-TiO₂ NPs have higher cell uptake efficiency than pure TiO₂, which made it possible to have higher selectivity and photocatalytic killing efficiency. The mechanism was that photocatalytic Fe₃O₄-TiO₂ NPs inhibit cancer cells by yielding reactive oxygen species (ROS). Then, Fe₃O₄-TiO₂ induced apoptosis, mediated by arresting cell cycle in G₀/G₁ phase, reducing mitochondrial membrane potential, and mitochondrial depolarization.
- photodynamic therapy,
- Fe₃O₄-TiO₂ nanoparticle,
- photocatalysis,
- mechanism,
- HepG2 cell
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Photocatalytic Killing Effect of Fe3O4-TiO2 Nanoparticles on Hepatoma Carcinoma Cells for Targeting Photodynamic Therapy

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通讯作者: 陈斌, bchen63@163.com

Photocatalytic Killing Effect of Fe₃O₄-TiO₂ Nanoparticles on Hepatoma Carcinoma Cells for Targeting Photodynamic Therapy