Citation: En-dong Zhang, Li-bing Liu, Feng-ting Lv, Shu Wang. Water-soluble Conjugated Polymers for Biosensor Applications[J]. Acta Polymerica Sinica, ;2018, (2): 186-197. doi: 10.11777/j.issn1000-3304.2018.17269 shu

Water-soluble Conjugated Polymers for Biosensor Applications

En-dong Zhang ^1,2 ,
Li-bing Liu ^1,2 ,
Feng-ting Lv ^1,2 ,
Shu Wang ^1,2,,

1.
Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190
2.
University of Chinese Academy of Sciences, Beijing 100049

Corresponding author: Shu Wang, wangshu@iccas.ac.cn
Received Date: 18 September 2017
Revised Date: 2 November 2017

In recent years, conjugated polymers (CPs) with characteristic and excellent photochemical and photophysical properties have become particularly popular in scientific research and are widely used for biological sensing. Conjugated polymers are characterized by a delocalized electronic structure along their backbones and exhibit electronic coupling between each optoelectronic segments. Owing to unique delocalized π-conjugated structure, CPs are endowed with distinguished optical properties, such as light-harvesting and amplification capability and high energy transfer efficiency. Water-soluble conjugated polymers (WSCPs) introduced with charged groups or selectively-recognition elements on the side chains can specifically diagnose through electrostatic interaction or specific binding with targets. In addition, WSCPs with diverse absorption and emission characteristics have been designed and synthesized by tuning the constitution and conformation of the backbones. In contrast with fluorescence proteins, small organic molecules and quantum dots, CPs with greatly unique properties have greater advantages that directed to sensing and imaging in biochemical and biomedical fields. This review article is aimed to summarize recent research progresses on applications of WSCPs as biosensors for the detection of a series of biological targets. Firstly, according to electrostatics interaction of CPs and targeted DNA, different novel strategies are used for detection of DNA mutations (section 2). Meanwhile, CPs modified with recognition moieties can be utilized to detect specific proteins (section 3). In addition, based on different interaction modes between CPs and cells and pathogens, identification and discrimination of microbial pathogens and cells are realized (section 4). CPs can also be used for cell imaging due to their advantageous optical properties (section 5). The structures, characteristics and advantages of CPs, as well as various nove lidentifications and detections strategies are also discussed. Through a variety of design and modification, novel CPs can be obtained and used for identification and detection, which is complementary to traditional CPs in biosensor and widens the range of biological applications. It is worth mentioning that, with the development of inter-discipline, more attention should be paid to the combination of new methods and strategies for exploiting functions of CPs in biological sensing.
- Conjugated polymer,
- Biosensor,
- Signal amplification,
- Identification and determination,
- Cell imaging
1. [1]
  Lakowicz J R, Koen P A, Szmacinski H, Gryczynski I, Kuśba J. J Fluoresc, 1994, 4(1):117-136 doi: 10.1007/BF01876666
2. [2]
  de Lange F, Cambi A, Huijbens R, de Bakker B, Rensen W, Garcia-Parajo M, van Hulst N, Figdor C G. J Cell Sci, 2001, 114:4153-4160
3. [3]
  Fernández-Suárez M, Ting A Y. Nat Rev Mol Cell Biol, 2008, 9:929-943 doi: 10.1038/nrm2531
4. [4]
  Weiss S. Science, 1999, 283(5408):1676-1683 doi: 10.1126/science.283.5408.1676
5. [5]
  Ge X, Leah Tolosa A, Rao G. Anal Chem, 2004, 76(5):1403-1410 doi: 10.1021/ac035063p
6. [6]
  Xia T, Li N, Fang X H. Annu Rev Phys Chem, 2013, 64:459-480 doi: 10.1146/annurev-physchem-040412-110127
7. [7]
  Nienhaus K, Nienhaus G U. Chem Soc Rev, 2014, 43:1088-1106 doi: 10.1039/C3CS60171D
8. [8]
  Rombouts K, Braeckmans K, Remaut K. Bioconjugate Chem, 2016, 27(2):280-297
9. [9]
  Zhou J, Yang Y, Zhang C Y. Chem Rev, 2015, 115:11669-11717 doi: 10.1021/acs.chemrev.5b00049
10. [10]
  Mei J, Leung N L C, Kwok R T K, Lam J W Y, Tang B Z. Chem Rev, 2015, 115:11718-11940 doi: 10.1021/acs.chemrev.5b00263
11. [11]
  Ito T, Shirakawa H, Ikeda S. J Polym Sci, Part A:Polym Chem, 1996, 34(13):2533-2542 doi: 10.1002/(ISSN)1099-0518
12. [12]
  Chiang C K, Fincher C R J, Park Y W, Heeger A J, Shirakawa H, Louis E J, Gau S C, MacDiarmid A G. Phys Rev Lett, 1977, 39(17):1098-1101 doi: 10.1103/PhysRevLett.39.1098
13. [13]
  Feng X, Liu L, Wang S, Zhu D. Chem Soc Rev, 2010, 39(7):2411-2419 doi: 10.1039/b909065g
14. [14]
  Zhu C L, Liu L B, Yang Q, Lv F T, Wang S. Chem Rev, 2012, 112:4687-4735 doi: 10.1021/cr200263w
15. [15]
  Yuan H X, Wang B, Lv F T, Liu L B, Wang S. Adv Mater, 2016, 26(40):6978-6982
16. [16]
  Zhang Jiangyan, Yuan Huanxiang, Zhu Chunlei, Liu Libing, Lv Fengting, Wang Shu. Scientia Sinca Chimica, 2016, 46(2):153-162
17. [17]
  Jiang Y F, McNeill J. Chem Rev, 2017, 117(2):838-859 doi: 10.1021/acs.chemrev.6b00419
18. [18]
  Swager T M, Acc Chem Res, 1998, 31(5):201-207
19. [19]
  Thomas S W, Joly G D, Swager T M. Chem Rev, 2007, 107(4):1339-1386 doi: 10.1021/cr0501339
20. [20]
  Dexter D L. J Chem Phys, 1953, 21:836-850 doi: 10.1063/1.1699044
21. [21]
  Forster T. Ann Phys, 1948, 2:55-58
22. [22]
  Zhan R Y, Liu B. Macromol Chem Phys, 2015, 216(2):131-144
23. [23]
  Rochat S, Swager T M. ACS Appl Mater Interfaces 2013, 5(11):4488-4502 doi: 10.1021/am400939w
24. [24]
  Wang J W, Lv F T, Liu L B, Ma Y G, Wang S. Coord Chem Rev, 2018, 354:135-154 doi: 10.1016/j.ccr.2017.06.023
25. [25]
  Heeger A J. Angew Chem Int Ed, 2001, 40(14):2591-2611 doi: 10.1002/(ISSN)1521-3773
26. [26]
  Liu B, Wang S, Bazan G C. J Am Chem Soc, 2003, 125(44):13306-13307 doi: 10.1021/ja0365072
27. [27]
  Feng L H, Zhu C L, Yuan H X, Liu L B, Wang S. Chem Soc Rev, 201342:6620-6633 doi: 10.1039/c3cs60036j
28. [28]
  Xu L G, Cheng L, Wang C, Peng R, Liu Z. Polym Chem, 2014, 5(5):1573-1580 doi: 10.1039/C3PY01196H
29. [29]
  Bai H T, Yuan H X, Nie C Y, Wang B, Lv F T, Liu L B, Wang S. Angew Chem Int Ed, 2015, 54(45):13208-13213
30. [30]
  Baylin S B, Ohm J E. Nat Rev Cancer, 2006, 6(2):107-116 doi: 10.1038/nrc1799
31. [31]
  Harris T J R, McCormick F. Nat Rev Clin Oncol, 2010, 7:251-265 doi: 10.1038/nrclinonc.2010.41
32. [32]
  Sanger F, Nicklen S, Coulson A R. Proc Natl Acad Sci, 1977, 74(12):104-108
33. [33]
  Ronaghi M, Uhlén M, Nyrén P. Science, 1998, 281(5375):363-365 doi: 10.1126/science.281.5375.363
34. [34]
  Samuels Y, Wang Z H, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, Gazdar A, Powell S M, Riggins G J, Willson J K V, Markowitz S, Kinzler K W, Vogelstein B, Velculescu V E. Science, 2004, 304(5670):554 doi: 10.1126/science.1096502
35. [35]
  Wittwer C T, Reed G H, Gundry C N, Vandersteen J G, Pryor R J. Clin Chem, 2003, 49(6):853-860 doi: 10.1373/49.6.853
36. [36]
  Inazuka M, Tahira T, Hayashi K. Genome Res, 1996, 6(6):551-557 doi: 10.1101/gr.6.6.551
37. [37]
  Board R E, Thelwell N J, Ravetto P F, Little S, Ranson M, Dive C, Hughes A, Whitcombe D. Clin Chem, 2008, 54(4):757-760 doi: 10.1373/clinchem.2007.098376
38. [38]
  Holland P M, Abramson R D, Watson R, Gelfand D H. Proc Natl Acad Sci, 1991, 88(16):7276-7280 doi: 10.1073/pnas.88.16.7276
39. [39]
  Hurst C D, Zuiverloon T C M, Hafner C, Zwarthoff E C, Knowles M A. BMC Res Notes, 2009, 2(1):66 doi: 10.1186/1756-0500-2-66
40. [40]
  Song J Z, Yang Q, Lv F T, Liu L B, Wang S. ACS Appl Mater Interfaces, 2012, 4(6):2885-2890 doi: 10.1021/am300830r
41. [41]
  Wang X Y, He F, Tang F, Li L D. J Mater Chem, 2012, 22(30):15303-15308 doi: 10.1039/c2jm32534a
42. [42]
  Song J Z, Zhang J Y, Lv F T, Cheng Y Q, Wang B, Feng L H, Liu L B, Wang S. Angew Chem Int Ed, 2013, 52(49):13020-13023
43. [43]
  Ehrlich M. Oncogene, 2002, 21(35):5400-5413 doi: 10.1038/sj.onc.1205651
44. [44]
  Esteller M, Herman J G. J Pathol, 2002, 196(1):1-7 doi: 10.1002/(ISSN)1096-9896
45. [45]
  Robertson K D. Oncogene, 2001, 20(24):3139-3155 doi: 10.1038/sj.onc.1204341
46. [46]
  Yang Q, Dong Y, Wu W, Zhu C L, Chong H, Lu J Y, Yu D H, Liu L B, Lv F T, Wang S. Nat Commun, 2012, 3:1206
47. [47]
  Zhang J Y, Xing B L, Song J Z, Nie C Y, Jiao L, Liu L B, Lv F T, Wang S. Anal Chem, 2014, 86(1):346-350 doi: 10.1021/ac402720g
48. [48]
  Feng F D, Liu L B, Yang Q, Wang S. Macromol Rapid Commun, 2010, 31(16):1405-1421 doi: 10.1002/marc.201000020
49. [49]
  Liu Xingfen, CaiXiaohui, Huang Yanqin, Shi Lin, Fan Quli, Huang Wei. Acta Chimica Sinica, 2014, 72(4):440-446
50. [50]
  Sun Pengfei, Fan Quli, Liu Lulin, Deng Weixing, Lu Xiaomei, Huang Wei. Acta Polymerica Sinica, 2014, (12):1629-1634
51. [51]
  Wang X Y, Li S L, Zhang P B, Lv F T, Liu L B, Wang S. Adv Mater, 2015, 27(39):6040-6045 doi: 10.1002/adma.201502880
52. [52]
  Imaoka M, Yano S, Okumura M, Hibi T, Wakayama M. Biosci Biotechnol Biochem, 2010, 74(9):1936-1939
53. [53]
  Ryan C J, Peng W, Kheoh T, Welkowsky E, Haqq C M, Chandler D W, Scher H I, Molina A. Prostate Cancer P D, 2014, 172(2):192-198
54. [54]
  Wen Q S, Zhu C L, Liu L B, Yang Q, Wang S, Zhu D B. Macromol Chem Phys, 2012, 213(23):2486-2491 doi: 10.1002/macp.v213.23
55. [55]
  Liu Xingfen, Hua Xiaoxiao, Huang Yanqin, Feng Xiaomiao, Fan Quli, Huang Wei. Chemistry Bulletin, 2015, 78(10):895-901
56. [56]
  Liu Xingfen, Wang Yateng, Huang Yanqin, Feng Xiaomiao, Fan Quli, Huang Wei. Acta Chimica Sinica, 2016, 74(8):664-668
57. [57]
  Gu P, Liu X F, Tian Y Y, Zhang L, HuangY Q, Su S, Feng X M, Fan Q L, Huang W. Sensors Actuat B-Chem, 2017, 246:78-84 doi: 10.1016/j.snb.2017.01.043
58. [58]
  Lazcka O, Del Campo F J, Munoz F X. Biosens Bioelectron, 2007, 22(7):1205-1217 doi: 10.1016/j.bios.2006.06.036
59. [59]
  Yuan H X, Liu Z, Liu L B, Lv F T, Wang Y L, Wang S. Adv Mater, 2014, 26(25):4333-4338 doi: 10.1002/adma.v26.25
60. [60]
  Bai H T, Chen H, Hu R, Li M, Lv F T, Liu L B, Wang S. ACS Appl Mater Interfaces, 2016, 8(46):31550-31557 doi: 10.1021/acsami.6b09807
61. [61]
  Nie C Y, Li S L, Wang B, Liu L B, Hu R, Chen H, Lv F T, Dai Z, Wang S. Adv Mater, 2016, 28(19):3749-3754 doi: 10.1002/adma.201600106
62. [62]
  Huang Y Q, Yao X, Zhang R, Ouyang L, Jiang R C, Liu X F, Song C X, Zhang G W, Fan Q L, Wang L H, Huang W. ACS Appl Mater Interfaces, 2014, 6(21):19144-19153 doi: 10.1021/am505113p
63. [63]
  Nie C Y, Wang B, Zhang J Y, Cheng Y Q, Lv F T, Liu L B, Wang S. Small, 2015, 11(21):2555-2563 doi: 10.1002/smll.v11.21
64. [64]
  Liu R H, Cui Q L, Wang C, Wang X Y, Yu Y, Li L D. ACS Appl Mater Interfaces, 2017, 9(3):3006-3015 doi: 10.1021/acsami.6b14320
65. [65]
  Song J Z, Lv F T, Yang G M, Liu L B, Yang Q, Wang S. Chem Commun, 2012, 48(60):7465-7467 doi: 10.1039/c2cc32085a
66. [66]
  Feng L H, Liu L B, Lv F T, Bazan G C, Wang S. Adv Mater, 2014, 26(23):3926-3930 doi: 10.1002/adma.201305206
67. [67]
  Xia B H, Wang X Y, He F, Cui Q L, Li L D. ACS Appl Mater Interfaces, 2012, 4(11):6332-6337 doi: 10.1021/am301945k
68. [68]
  Wang B, Zhu C L, Liu L B, Lv F T, Yang Q, Wang S. Polym Chem, 2013, 4(20):5212-5215 doi: 10.1039/c3py00097d
69. [69]
  Cui Q L, Wang X Y, Yang Y, Li S L, Li L D, Wang S. Chem Mater, 2016, 28(13):4661-4669 doi: 10.1021/acs.chemmater.6b01424
70. [70]
  Wang F Y, Liu Z, Wang B, Feng L H, Liu L B, Lv F T, Wang Y L, Wang S. Angew Chem, 2014, 126(2):434-438 doi: 10.1002/ange.v126.2
71. [71]
  Wang Y X, Li S L, Feng L H, Nie C Y, Liu L B, Lv F T, Wang S. ACS Appl Mater Interfaces, 2015, 7(43):24110-24118 doi: 10.1021/acsami.5b07172
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