Citation: Song Tao, Xi Yue-jing, Du Jian-zhong. Antibacterial Hydrogels Incoporated with Poly(glutamic acid)-based Vesicles[J]. Acta Polymerica Sinica, ;2018, (1): 119-128. doi: 10.11777/j.issn1000-3304.2018.17229 shu

Antibacterial Hydrogels Incoporated with Poly(glutamic acid)-based Vesicles

Key Laboratory of Advanced Civil Engineering Materials of the Ministry of Education, School of Material Science and Engineering, Tongji University, Shanghai 201804

Corresponding author: Du Jian-zhong, jzdu@tongji.edu.cn
Received Date: 17 August 2017
Revised Date: 13 September 2017

We designed a hydrogel with durable and excellent antibacterial activity. Amphiphilic block copolymer, polycaprolactone-block-poly(glutamic acid) (PCL-b-PGA), was first synthesized by ring-opening polymerization with amine-terminated polycaprolactone (PCL-NH₂) as the macro-initiator and Bz-Glu-NCA as the monomer. This copolymer self-assembled into vesicles in solvent mixture of DMF/water with a critical vesiculation concentration of 5.27 μg mL^-1. Antibacterial Ag-decorated vesicles were then prepared by in situ deposition of silver nanoparticles in PGA coronas of the vesicles as a result of the electrostatic interaction between carboxyl groups and Ag⁺. ¹H-NMR analysis revealed the composition of PCL₄₇-b-PGA₄₆ block copolymer. Dynamic light scattering (DLS) study revealed an intensity-averaged diameter of 215 nm for the vesicles with a polydispersity of 0.029. Transmission electron microscopy (TEM) confirmed a hollow structure of the vesicles with a number-averaged diameter of 270 nm, and the presence of sliver nanoparticles on the vesicles. Atomic force microscopy (AFM) revealed the collapsed surface morphology of vesicles with a width to height ratio of 18, confirming further the hollow structure of the vesicles. Meanwhile, Ag-decorated vesicles were incoporated into Pluronic F127 hydrogels to afford antibacterial hydrogels, showing good degradability at different pH. MIC₉₀(minimum concentration of inhibiting 90% of bacterium) of the antibacterial vesicles was 10 μg mL^-1 against Gram-negative bacterium E. coli, and it was 20 μg mL^-1 against Gram-positive bacterium S. aureus, and measured by spread plate method, which afford the hydrogels excellent antibacterial property. Oxford cup tests confirmed that MIC₅₀ of the antibacterial hydrogel against both E. coli and S. aureus is 7.5 μg mL^-1 and MBC (minimum bactericidal concentration) against both of them is 30 μg mL^-1. The in vitro degradation tests confirmed that the vesicle was degraded in the presence of lipase. Overall, we provide a new method for preparation of antibacterial hydrogels, which may have promising biomedical applications requiring a long-acting antibacterial therapy such as inflammation triggered by bacteria infection, wound healing after surgery and sterilization for the implant devices, etc.
- Vesicles,
- Poly(amino acid)s,
- Antibacterial property,
- Hydrogels,
- Self-assembly
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