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Citation: Qing-hua Yang, Qiang-sheng Wang, Long-gang Wang, Tian-yun Chen. Synthesis and Property Evaluations of a Nonfouling Polypeptide[J]. Acta Polymerica Sinica, ;2016, (4): 503-510. doi: 10.11777/j.issn1000-3304.2016.15250 shu

Synthesis and Property Evaluations of a Nonfouling Polypeptide

1.
Department of Pharmaceutical Engineering, School of Medical Engineering, Hefei University of Technology, Hefei 230009
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Department of Chemical Technology, School of Environmental and Chemical Engineering, Yanshan University, Qinghuangdao 066004
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Department of Chemical Technology, School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei 230009

Received Date: 14 September 2015
Revised Date: 11 November 2015

A nonfouling polypeptide(Poly(D)-K) with poly(aspartic acid) backbone and lysine side chains was developed by polycondensing a covalently bonded dimer of aspartic acid (D) and lysine (K).Gel permeation chromatography (GPC), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy(XPS), circular dichroism (CD) were conducted to determine the molecular weight, structure, secondary structure of the polypeptide and compositions of self-assembling monolayers (SAMs) of the polypeptide on gold chips, respectively.The results show that the polypeptide was successfully prepared, of which the average molecular weight (Mw) is about 3.8103, polydispersity index (PDI) is 1.33, average degree of polymerization (DP) is about 12.4; and secondary conformation of the polypeptide in water solution is random coil.The polypeptide could form SAMs on gold chips by the terminal thiol groups, and the lowest relatively nonspecific protein adsorption on SAMs surface determined by Enzyme-linked Immunosorbent Assay (ELISA) for anti-IgG and Fg was (8.53.6)% and (12.54.8)%, respectively, where the protein adsorption on tissue culture polystyrene (TCPS) surface was set as 100%.The cell viability of the polypeptide determined by MTT assay was (87.64.3)% even its concentration was up to 5 mg/mL.
- Polypeptide,
- Nonfouling,
- Biocompatibility,
- Self-assembling monolayers(SAMs)
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