Effects of Chirality on Gene Delivery Efficiency of Polylysine

Bin-wei Zhao Zhuxian Zhou Youqing Shen

Citation:  Bin-wei Zhao, Zhuxian Zhou, Youqing Shen. Effects of Chirality on Gene Delivery Efficiency of Polylysine[J]. Chinese Journal of Polymer Science, 2016, 34(1): 94-103. doi: 10.1007/s10118-016-1735-2 shu

Effects of Chirality on Gene Delivery Efficiency of Polylysine

    通讯作者: Youqing Shen,
  • 基金项目:

    This work was financially supported by the National Basic Research Program (No. 2014CB931900) and the National Natural Science Foundation of China (No. 21090352).

摘要: Chirality is a key factor in the biological activity of many biomolecules. Poly(L-lysine)(PLL), a polypeptide synthesized from L-lysine, is one of the mostly used cationic polymers for gene delivery. The effect of chirality of polylysine (PL) on its gene delivery remains unknown. Herein, we prepared three polylysines (PLs) with the similar molecular weight but different backbone chiralities including poly(L-lysine)(PLL), poly(D-lysine)(PDL) and poly(DL-lysine)(PDLL). The side chains of each PL were modified with propylene oxide (PO) of different chiralities including (R)PO, (S)PO and (R,S)PO. These PL-POs with distinct chirality in main and side chains could condense pDNA into polyplexes. The polyplexes had approximately the same size, zeta potential and binding ability, but showed distinct gene transfection efficiency. We found that the PLs of L-configuration in the main chain had higher transfection efficiency than that of D or DL configuration due to their faster cellular uptake, while the side chain chirality had no effect on transfection efficiency.

English

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  • 发布日期:  2016-01-05
  • 收稿日期:  2015-07-13
  • 修回日期:  2015-08-17
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