Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

Citation: Qi-jia-yu Wu, Rui Wang, Ying Zhou, Ya-qin Huang, Raja Ghosh, Xiao-nong Chen. Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation[J]. Chinese Journal of Polymer Science, 2015, 33(7): 1048-1057. doi: 10.1007/s10118-015-1655-6 shu

Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

1.
1. Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
2.
2. Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada
3.
4.
Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
5.
Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada
基金项目:
This work was financially supported by the China Scholarship Council and the National Natural Science Foundation of China (No. 20874004).

摘要: Thermal and salt dual stimuli-responsive filter-paper-based membranes were prepared by UV-induced grafting of NIPAM-based polymers on paper surface. The grafting ratio could be controlled by monomer concentration during grafting polymerization. The results from pressure drop measurement of the mobile phase flowed cross the membrane demonstrate that an appropriate grafting ratio would be 8%-10%. Protein adsorption on the membrane through hydrophobic interaction could be promoted by increasing temperature and lyotropic salt concentration. The effect of grafted polymer structure on protein binding performance was studied. Filter paper grafted with NIPAM-based branched copolymer consisting of hydrophobic monomer moieties shows ten times higher protein binding capacity than that of the original filter paper. The separation of plasma proteins using the dual stimuli-responsive membrane was examined to demonstrate feasible application for hydrophobic interaction chromatographic separation of proteins.

关键词:

N-isopropyl acrylamide
/ Filter paper
/ Surface grafting
/ Stimuli-responsive hydrophobic transition
/ Protein separation

English

Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

1.
1. Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
2.
2. Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada
3.
4.
Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
5.
Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada
Received Date: 06 November 2014
Revised Date: 20 December 2014
Available Online: 05 July 2015
Fund Project:
This work was financially supported by the China Scholarship Council and the National Natural Science Foundation of China (No. 20874004).

Abstract: Thermal and salt dual stimuli-responsive filter-paper-based membranes were prepared by UV-induced grafting of NIPAM-based polymers on paper surface. The grafting ratio could be controlled by monomer concentration during grafting polymerization. The results from pressure drop measurement of the mobile phase flowed cross the membrane demonstrate that an appropriate grafting ratio would be 8%-10%. Protein adsorption on the membrane through hydrophobic interaction could be promoted by increasing temperature and lyotropic salt concentration. The effect of grafted polymer structure on protein binding performance was studied. Filter paper grafted with NIPAM-based branched copolymer consisting of hydrophobic monomer moieties shows ten times higher protein binding capacity than that of the original filter paper. The separation of plasma proteins using the dual stimuli-responsive membrane was examined to demonstrate feasible application for hydrophobic interaction chromatographic separation of proteins.

Key words:

N-isopropyl acrylamide
/ Filter paper
/ Surface grafting
/ Stimuli-responsive hydrophobic transition
/ Protein separation

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Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

English

Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

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Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

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Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

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