引用本文:
Zhi Bo CHEN, Bo Xun ZHANG, Zhong Xiu HUANG, Qing Lin PENG, Jia CHEN, Yu WANG, Jian Guo ZHANG, Guang Zhi JIANG, Wen Shu LI, Lan Ying LIU. Synthesis and Kinetics of a Novel Mimic with Glutathione Peroxidase Activity-Tellurium-containing Hyaluronic Acid (TeHA)[J]. Chinese Chemical Letters,
2006, 17(7): 969-972.
Citation: Zhi Bo CHEN, Bo Xun ZHANG, Zhong Xiu HUANG, Qing Lin PENG, Jia CHEN, Yu WANG, Jian Guo ZHANG, Guang Zhi JIANG, Wen Shu LI, Lan Ying LIU. Synthesis and Kinetics of a Novel Mimic with Glutathione Peroxidase Activity-Tellurium-containing Hyaluronic Acid (TeHA)[J]. Chinese Chemical Letters, 2006, 17(7): 969-972.
Citation: Zhi Bo CHEN, Bo Xun ZHANG, Zhong Xiu HUANG, Qing Lin PENG, Jia CHEN, Yu WANG, Jian Guo ZHANG, Guang Zhi JIANG, Wen Shu LI, Lan Ying LIU. Synthesis and Kinetics of a Novel Mimic with Glutathione Peroxidase Activity-Tellurium-containing Hyaluronic Acid (TeHA)[J]. Chinese Chemical Letters, 2006, 17(7): 969-972.
Synthesis and Kinetics of a Novel Mimic with Glutathione Peroxidase Activity-Tellurium-containing Hyaluronic Acid (TeHA)
摘要:
A novel mimic was synthesized by modifying hyaluronic acid (HA) with tellurium,whose function is similar to that of glutathione peroxidase (GPX). The structure of TeHA was characterized by means of IR and NMR, the target-Te was located at -CH2OH of the N-acetyl-D-glucosamine of HA. The H2O2 reducing activity of TeHA, by glutathione (GSH), was 163.6 U/μmol according to Wilson's method. In contrast to other mimics, TeHA displayed the highest activity. Moreover, TeHA accepted many hydroperoxides as its substrates, such as H2O2, cumenyl hydroperoxide (CuOOH) and tert-butyl hydroperoxide (t-BuOOH), and CuOOH was the optimal substrate of TeHA. A ping-pong mechanism was observed in the steady-state kinetic studies of the reactions catalyzed by TeHA.
-
关键词:
- Hyaluronic acid
- / tellurium
- / glutathione peroxidase
- / synthesis
- / kinetics
English
Synthesis and Kinetics of a Novel Mimic with Glutathione Peroxidase Activity-Tellurium-containing Hyaluronic Acid (TeHA)
Abstract:
A novel mimic was synthesized by modifying hyaluronic acid (HA) with tellurium,whose function is similar to that of glutathione peroxidase (GPX). The structure of TeHA was characterized by means of IR and NMR, the target-Te was located at -CH2OH of the N-acetyl-D-glucosamine of HA. The H2O2 reducing activity of TeHA, by glutathione (GSH), was 163.6 U/μmol according to Wilson's method. In contrast to other mimics, TeHA displayed the highest activity. Moreover, TeHA accepted many hydroperoxides as its substrates, such as H2O2, cumenyl hydroperoxide (CuOOH) and tert-butyl hydroperoxide (t-BuOOH), and CuOOH was the optimal substrate of TeHA. A ping-pong mechanism was observed in the steady-state kinetic studies of the reactions catalyzed by TeHA.
-
Key words:
- Hyaluronic acid
- / tellurium
- / glutathione peroxidase
- / synthesis
- / kinetics
扫一扫看文章
计量
- PDF下载量: 0
- 文章访问数: 0
- HTML全文浏览量: 0
下载:
