引用本文:
Shao Ming YANG, Yang Mei LI, Xiu Ming JIANG, Xian Fu LIN. A Novel Reagentless Biosensor Constructed by Layer-by-Layer Assembly of HRP and Nile Blue Premixed with Polyanion[J]. Chinese Chemical Letters,
2005, 16(7): 983-986.
Citation: Shao Ming YANG, Yang Mei LI, Xiu Ming JIANG, Xian Fu LIN. A Novel Reagentless Biosensor Constructed by Layer-by-Layer Assembly of HRP and Nile Blue Premixed with Polyanion[J]. Chinese Chemical Letters, 2005, 16(7): 983-986.
Citation: Shao Ming YANG, Yang Mei LI, Xiu Ming JIANG, Xian Fu LIN. A Novel Reagentless Biosensor Constructed by Layer-by-Layer Assembly of HRP and Nile Blue Premixed with Polyanion[J]. Chinese Chemical Letters, 2005, 16(7): 983-986.
A Novel Reagentless Biosensor Constructed by Layer-by-Layer Assembly of HRP and Nile Blue Premixed with Polyanion
摘要:
A novel reagentless biosensor constructed by the organic dye nile blue (NB) and horseradish peroxidase (HRP) has been fabricated via layer-by-layer (LBL) self-assembly technique. NB premixed with polyanion poly (sodium-p-styrenesulfonate) (PSS) acts as the mediator between the immobilized HRP and the electrode surface. The response of the biosensor to hydrogen peroxide has been investigated. The linear range of the biosensor to hydrogen peroxide was from 0.20 mmol/L to 7.03 mmol/L with a sensitivity of 8.45 μA/(mmol/L).
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关键词:
- Nile blue
- / premixed
- / layer-by-layer
- / horseradish peroxidase (HRP)
- / biosensor
English
A Novel Reagentless Biosensor Constructed by Layer-by-Layer Assembly of HRP and Nile Blue Premixed with Polyanion
Abstract:
A novel reagentless biosensor constructed by the organic dye nile blue (NB) and horseradish peroxidase (HRP) has been fabricated via layer-by-layer (LBL) self-assembly technique. NB premixed with polyanion poly (sodium-p-styrenesulfonate) (PSS) acts as the mediator between the immobilized HRP and the electrode surface. The response of the biosensor to hydrogen peroxide has been investigated. The linear range of the biosensor to hydrogen peroxide was from 0.20 mmol/L to 7.03 mmol/L with a sensitivity of 8.45 μA/(mmol/L).
-
Key words:
- Nile blue
- / premixed
- / layer-by-layer
- / horseradish peroxidase (HRP)
- / biosensor
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