Analysis of Toxin β-N-Oxalyl-α,β-diaminopropionic Acid (β-ODAP),its Isomer α-ODAP and Other Free Amino Acids in <i>Lathyrus sativus</i>

引用本文: Ze Yi YAN, Cheng Jin JIAO, Fen Min LI, Yong Min LIANG, Zhi Xiao LI. Analysis of Toxin β-N-Oxalyl-α,β-diaminopropionic Acid (β-ODAP),its Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus[J]. Chinese Chemical Letters, 2005, 16(5): 627-630. shu

Citation: Ze Yi YAN, Cheng Jin JIAO, Fen Min LI, Yong Min LIANG, Zhi Xiao LI. Analysis of Toxin β-N-Oxalyl-α,β-diaminopropionic Acid (β-ODAP),its Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus[J]. Chinese Chemical Letters, 2005, 16(5): 627-630. shu

Analysis of Toxin β-N-Oxalyl-α,β-diaminopropionic Acid (β-ODAP),its Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus

1.
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000;
2.
School of life science, Lanzhou University, Lanzhou 730000
基金项目:
This work was supported by NKBRSF Project (G2000018603) and the National Natural Science Foundation of China (No. 30270965).

摘要: A method has been presented for the simultaneous analysis of neurotoxin β-N-oxalyl-α,β-diaminopropionic acid (β-ODAP) and its non-or low-toxic isomer α-ODAP as well as other amino acids in Lathyrus sativus extracts after derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-C1) by reversed-phase (C₁₈ column) high performance liquid chromatography(RP-HPLC). Detection limits of isomers were 0.08 μg/mL for both β-, α-ODAP, for homoarginine 0.09 μg/mL at a signal-to-noise ratio of 3:1.

关键词:

HPLC
/ β-N-oxalyl-L-α,β-diaminopropanoic acid
/ α-ODAP
/ homoarginine
/ PNZ-Cl

English

Analysis of Toxin β-N-Oxalyl-α,β-diaminopropionic Acid (β-ODAP),its Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus

1.
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000;
2.
School of life science, Lanzhou University, Lanzhou 730000
Received Date: 13 April 2004
Fund Project:
This work was supported by NKBRSF Project (G2000018603) and the National Natural Science Foundation of China (No. 30270965).

Abstract: A method has been presented for the simultaneous analysis of neurotoxin β-N-oxalyl-α,β-diaminopropionic acid (β-ODAP) and its non-or low-toxic isomer α-ODAP as well as other amino acids in Lathyrus sativus extracts after derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-C1) by reversed-phase (C₁₈ column) high performance liquid chromatography(RP-HPLC). Detection limits of isomers were 0.08 μg/mL for both β-, α-ODAP, for homoarginine 0.09 μg/mL at a signal-to-noise ratio of 3:1.

Key words:

HPLC
/ β-N-oxalyl-L-α,β-diaminopropanoic acid
/ α-ODAP
/ homoarginine
/ PNZ-Cl

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of Toxin β-N-Oxalyl-α,β-diaminopropionic Acid (β-ODAP),its Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus

English

Analysis of Toxin β-N-Oxalyl-α,β-diaminopropionic Acid (β-ODAP),its Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

Analysis of Toxin β-N-Oxalyl-α,β-diaminopropionic Acid (β-ODAP),its Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus

English

Analysis of Toxin β-N-Oxalyl-α,β-diaminopropionic Acid (β-ODAP),its Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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