引用本文:
Yan WANG, Bo Lin GONG, Xin Du GENG. Refolding of Denatured/Reduced Lysozyme Using Weak-Cation Exchange Chromatography[J]. Chinese Chemical Letters,
2003, 14(8): 828-831.
Citation: Yan WANG, Bo Lin GONG, Xin Du GENG. Refolding of Denatured/Reduced Lysozyme Using Weak-Cation Exchange Chromatography[J]. Chinese Chemical Letters, 2003, 14(8): 828-831.
Citation: Yan WANG, Bo Lin GONG, Xin Du GENG. Refolding of Denatured/Reduced Lysozyme Using Weak-Cation Exchange Chromatography[J]. Chinese Chemical Letters, 2003, 14(8): 828-831.
Refolding of Denatured/Reduced Lysozyme Using Weak-Cation Exchange Chromatography
摘要:
Oxidative refolding of the denatured/reduced lysozyme was investigated by using weak-cation exchange chromatography (WCX). The stationary phase of WCX binds to the reduced lysozyme and prevented it from forming intermolecular aggregates. At the same time urea and ammonium sulfate were added to the mobile phase to increase the elution strength for lysozyme. Ammonium sulfate can more stabilize the native protein than a common eluting agent, sodium chloride. Refolding of lysozyme by using this WCX is successfully. It was simply carried out to obtain a completely and correctly refolding of the denatured lysozyme at high concentration of 20.0 mg/mL.
English
Refolding of Denatured/Reduced Lysozyme Using Weak-Cation Exchange Chromatography
Abstract:
Oxidative refolding of the denatured/reduced lysozyme was investigated by using weak-cation exchange chromatography (WCX). The stationary phase of WCX binds to the reduced lysozyme and prevented it from forming intermolecular aggregates. At the same time urea and ammonium sulfate were added to the mobile phase to increase the elution strength for lysozyme. Ammonium sulfate can more stabilize the native protein than a common eluting agent, sodium chloride. Refolding of lysozyme by using this WCX is successfully. It was simply carried out to obtain a completely and correctly refolding of the denatured lysozyme at high concentration of 20.0 mg/mL.
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Key words:
- Weak-cation exchange chromatography
- / lysozyme
- / protein refolding
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