In this study, a self-calibrating near-infrared fluorescence probe was designed and synthesized based on the dual-fluorophore strategy utilizing methylene blue and coumarin. The probe utilized methylene blue (emission spectrum range: 640-740 nm) and coumarin fluorophore (emission spectrum range: 440-600 nm) as signal output units, thereby achieving effective spectral separation and highly selective detection of HClO. Under physiological pH conditions, HClO triggers an oxidation-cleavage reaction, releasing methylene blue and coumarin, which emit distinct red and green fluorescence, respectively. This dualemission feature enabled rapid HClO detection with two-channel detection limits of 25.13 nmol·L^-1 (green channel) and 31.55 nmol·L^-1 (red channel). Furthermore, in cell imaging experiments, this probe demonstrated excellent cell membrane permeability and low cytotoxicity, successfully enabling the monitoring of both endogenous and exogenous HClO in living cells. By incorporating a two-channel self-calibration system, the probe effectively mitigated signal variations caused by instrumental or environmental interference, substantially improving detection sensitivity and reliability.

采用高温固相法制备了Ca_0.993-yScBO₄∶0.007Bi³⁺，yEu³⁺(y=0~0.100)系列荧光粉。通过在CaScBO₄基质中构建高效的Bi³⁺→Eu³⁺非辐射能量传递体系，成功实现了330 nm激发下发光颜色从蓝光区[CIE色坐标：(0.153，0.050)]到橙红光区[CIE色坐标：(0.530，0.270)]的连续调谐。该荧光粉的能量传递效率最高可达71.2%，机制为电偶极-电偶极相互作用，临界距离为1.36 nm。代表性橙红光样品Ca_0.893ScBO₄∶0.007Bi³⁺, 0.100Eu³⁺在423 K时仍保持室温积分强度的77.7%，热猝灭活化能为0.417 eV，表现出优异的热稳定性。相较于单掺的Ca_0.993ScBO₄∶0.007Bi³⁺蓝光荧光粉，该共掺体系在维持较高量子效率与优异热稳定性的同时，实现了蓝光至橙红光的宽范围颜色调谐。