引用本文:
Dan Dan Wang, Hai Yan Wang, Ye Hong Zhou, Chun Gui Zhao, Chuan Dong, Shao Min Shuang. Study on the effects of cefotaxime on intracellular Ca2+ in human peripheral lymphocytes by fluoremetry[J]. Chinese Chemical Letters,
2007, 18(4): 424-426.
doi:
10.1016/j.cclet.2006.12.044
Citation: Dan Dan Wang, Hai Yan Wang, Ye Hong Zhou, Chun Gui Zhao, Chuan Dong, Shao Min Shuang. Study on the effects of cefotaxime on intracellular Ca2+ in human peripheral lymphocytes by fluoremetry[J]. Chinese Chemical Letters, 2007, 18(4): 424-426. doi: 10.1016/j.cclet.2006.12.044
Citation: Dan Dan Wang, Hai Yan Wang, Ye Hong Zhou, Chun Gui Zhao, Chuan Dong, Shao Min Shuang. Study on the effects of cefotaxime on intracellular Ca2+ in human peripheral lymphocytes by fluoremetry[J]. Chinese Chemical Letters, 2007, 18(4): 424-426. doi: 10.1016/j.cclet.2006.12.044
Study on the effects of cefotaxime on intracellular Ca2+ in human peripheral lymphocytes by fluoremetry
摘要:
Characteristic of Fura-2-Ca2+ interaction was studied based on the fluorescence technique. The apparent dissociation constants (Kd) of the Fura-2-Ca2+ complex were determined at different temperature. The effect of cefotaxime (CEFA) on intracellular Ca2+ concentration ([Ca2+]i) was discussed by using a ratiometric fluorescence dye Fura-2 as a probe. The basal [Ca2+]i in resting human peripheral lymphocytes was 100±7 nmol/L but after treatment with cefotaxime, the changes of [Ca2+]i were observed in different conditions. In the concentration range of 1-30 μmol/L of cefotaxime [Ca2+]i increased, as a result of releasing intracellular Ca2+ stores. Higher concentration of cefotaxime (50-500 μmol/L) stimulated to decrease of [Ca2+]i.
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关键词:
- Fura-2
- / Lymphocytes
- / Cefotaxime
- / [Ca2+]i
English
Study on the effects of cefotaxime on intracellular Ca2+ in human peripheral lymphocytes by fluoremetry
Abstract:
Characteristic of Fura-2-Ca2+ interaction was studied based on the fluorescence technique. The apparent dissociation constants (Kd) of the Fura-2-Ca2+ complex were determined at different temperature. The effect of cefotaxime (CEFA) on intracellular Ca2+ concentration ([Ca2+]i) was discussed by using a ratiometric fluorescence dye Fura-2 as a probe. The basal [Ca2+]i in resting human peripheral lymphocytes was 100±7 nmol/L but after treatment with cefotaxime, the changes of [Ca2+]i were observed in different conditions. In the concentration range of 1-30 μmol/L of cefotaxime [Ca2+]i increased, as a result of releasing intracellular Ca2+ stores. Higher concentration of cefotaxime (50-500 μmol/L) stimulated to decrease of [Ca2+]i.
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Key words:
- Fura-2
- / Lymphocytes
- / Cefotaxime
- / [Ca2+]i
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