Production of Polyclonal Antibody of Morphine and Determination of Morphine in Urine by Capillary Electrophoresis Immunoassay with Laser-induced Fluorescence Detection

Jian Qiu MI Xiao Hua QI Xin Xiang ZHANG Wen Bao CHANG

引用本文: Jian Qiu MI,  Xiao Hua QI,  Xin Xiang ZHANG,  Wen Bao CHANG. Production of Polyclonal Antibody of Morphine and Determination of Morphine in Urine by Capillary Electrophoresis Immunoassay with Laser-induced Fluorescence Detection[J]. Chinese Chemical Letters, 2004, 15(8): 943-946. shu
Citation:  Jian Qiu MI,  Xiao Hua QI,  Xin Xiang ZHANG,  Wen Bao CHANG. Production of Polyclonal Antibody of Morphine and Determination of Morphine in Urine by Capillary Electrophoresis Immunoassay with Laser-induced Fluorescence Detection[J]. Chinese Chemical Letters, 2004, 15(8): 943-946. shu

Production of Polyclonal Antibody of Morphine and Determination of Morphine in Urine by Capillary Electrophoresis Immunoassay with Laser-induced Fluorescence Detection

  • 基金项目:

    This work was financially supported by the National Natural Science Foundation of China (NNSFC) (20075001,20075003) and the Excellent Young Teachers Program of Ministry of Education, China. Some organic synthesis was completed in the lab of Prof. Jian-Bo Wang and thanks were also given here.

摘要: N-Conjugated antigen was synthesized and polyclonal antibody with high specificity was obtained from immunizing animals.With this polyclonal antibody, a rapid and efficient CEIA-LIF method was developed to determine the free morphine in urine of abusers.The detection limit was calculated to be 40 ng/mL.Simulated urine samples were analyzed with good recoveries, which showed the feasibility of its application in specific morphine determination in urine of morphine abusers.

English

  • 加载中
计量
  • PDF下载量:  3
  • 文章访问数:  1857
  • HTML全文浏览量:  46
文章相关
  • 收稿日期:  2003-07-21
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

/

返回文章