引用本文:
Yan Wang, Zhi Hua Chai, Hong Yan He, Yi Zhe Jiang, Guo Qi Fu. Polyacrylamide hydrogels with phenylboronic acid moieties for the imprinting of proteins[J]. Chinese Chemical Letters,
2010, 21(12): 1487-1489.
doi:
10.1016/j.cclet.2010.06.005
Citation: Yan Wang, Zhi Hua Chai, Hong Yan He, Yi Zhe Jiang, Guo Qi Fu. Polyacrylamide hydrogels with phenylboronic acid moieties for the imprinting of proteins[J]. Chinese Chemical Letters, 2010, 21(12): 1487-1489. doi: 10.1016/j.cclet.2010.06.005
Citation: Yan Wang, Zhi Hua Chai, Hong Yan He, Yi Zhe Jiang, Guo Qi Fu. Polyacrylamide hydrogels with phenylboronic acid moieties for the imprinting of proteins[J]. Chinese Chemical Letters, 2010, 21(12): 1487-1489. doi: 10.1016/j.cclet.2010.06.005
Polyacrylamide hydrogels with phenylboronic acid moieties for the imprinting of proteins
摘要:
In this letter, N-acryloyl-3-aminophenylboronic acid (AAPBA) was synthesized and then examined as a new functional monomer for protein imprinting. It was allowed to be copolymerized with acrylamide to produce hemoglobin-or lysozyme-imprinted hydrogels. In template rebinding tests, the imprinted gels showed significant increase in the specific binding with the increase of the AAPBA amounts in the prepolymerization recipes. These results indicate that AAPBA may be a useful functional monomer for its moderate interactions with protein molecules.
English
Polyacrylamide hydrogels with phenylboronic acid moieties for the imprinting of proteins
Abstract:
In this letter, N-acryloyl-3-aminophenylboronic acid (AAPBA) was synthesized and then examined as a new functional monomer for protein imprinting. It was allowed to be copolymerized with acrylamide to produce hemoglobin-or lysozyme-imprinted hydrogels. In template rebinding tests, the imprinted gels showed significant increase in the specific binding with the increase of the AAPBA amounts in the prepolymerization recipes. These results indicate that AAPBA may be a useful functional monomer for its moderate interactions with protein molecules.
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Key words:
- Protein imprinting
- / Hydrogels
- / Hemoglobin
- / Lysozyme
- / 3-Aminophenylboronic acid
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