引用本文:
Fu Yong Zhao, Zong Hua Wang, Hui Wang, Rui Zhao, Ming Yu Ding. Determination of uric acid in human urine by ion chromatography with conductivity detector[J]. Chinese Chemical Letters,
2011, 22(3): 342-345.
doi:
10.1016/j.cclet.2010.10.008
Citation: Fu Yong Zhao, Zong Hua Wang, Hui Wang, Rui Zhao, Ming Yu Ding. Determination of uric acid in human urine by ion chromatography with conductivity detector[J]. Chinese Chemical Letters, 2011, 22(3): 342-345. doi: 10.1016/j.cclet.2010.10.008
Citation: Fu Yong Zhao, Zong Hua Wang, Hui Wang, Rui Zhao, Ming Yu Ding. Determination of uric acid in human urine by ion chromatography with conductivity detector[J]. Chinese Chemical Letters, 2011, 22(3): 342-345. doi: 10.1016/j.cclet.2010.10.008
Determination of uric acid in human urine by ion chromatography with conductivity detector
摘要:
A simple, fast, precise and eco-friendly analytical method for the determination of uric acid (UA) in human urine by ion chromatography (IC) was established. The sample pretreatment was not required, only needed centrifugation and filtration. The separation was carried out on a cation exchange column with 2.0 mmol/L nitric acid as mobile phase at the flow-rate 1.0 mL/min. A non-suppressed conductivity detector was used. The IC analysis time for one run was within 10 min under the optimized IC condition. The detection limits were 0.5 μg/L(S/N=3) for uric acid. The recovery was 100.1% while the relative standard deviation (RSD) was 1.8% from 10 measurements.
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关键词:
- Ion chromatography
- / Uric acid
- / Urine
English
Determination of uric acid in human urine by ion chromatography with conductivity detector
Abstract:
A simple, fast, precise and eco-friendly analytical method for the determination of uric acid (UA) in human urine by ion chromatography (IC) was established. The sample pretreatment was not required, only needed centrifugation and filtration. The separation was carried out on a cation exchange column with 2.0 mmol/L nitric acid as mobile phase at the flow-rate 1.0 mL/min. A non-suppressed conductivity detector was used. The IC analysis time for one run was within 10 min under the optimized IC condition. The detection limits were 0.5 μg/L(S/N=3) for uric acid. The recovery was 100.1% while the relative standard deviation (RSD) was 1.8% from 10 measurements.
-
Key words:
- Ion chromatography
- / Uric acid
- / Urine
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