引用本文:
Zhi Ping Zhang, Yi Ming Li, Xiao Yun Chen, Qing Xiang Guo. Photoregulation of protein plasmid expression in vitro and in vivo using BHQ caging group[J]. Chinese Chemical Letters,
2011, 22(3): 338-341.
doi:
10.1016/j.cclet.2010.10.007
Citation: Zhi Ping Zhang, Yi Ming Li, Xiao Yun Chen, Qing Xiang Guo. Photoregulation of protein plasmid expression in vitro and in vivo using BHQ caging group[J]. Chinese Chemical Letters, 2011, 22(3): 338-341. doi: 10.1016/j.cclet.2010.10.007
Citation: Zhi Ping Zhang, Yi Ming Li, Xiao Yun Chen, Qing Xiang Guo. Photoregulation of protein plasmid expression in vitro and in vivo using BHQ caging group[J]. Chinese Chemical Letters, 2011, 22(3): 338-341. doi: 10.1016/j.cclet.2010.10.007
Photoregulation of protein plasmid expression in vitro and in vivo using BHQ caging group
摘要:
Green fluorescent protein (GFP) plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore (BHQ) for controlling its expression with exact spatiotemporal resolution. In vitro and in vivo experiments clearly verified that, comparing with Bhc caging, the expression level of caged GFP plasmid was dramatically decreased and then efficiently restored after subsequent photolysis.
English
Photoregulation of protein plasmid expression in vitro and in vivo using BHQ caging group
Abstract:
Green fluorescent protein (GFP) plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore (BHQ) for controlling its expression with exact spatiotemporal resolution. In vitro and in vivo experiments clearly verified that, comparing with Bhc caging, the expression level of caged GFP plasmid was dramatically decreased and then efficiently restored after subsequent photolysis.
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Key words:
- Photoregulation
- / Caging(uncaging)
- / BHQ
- / Green fluorescent protein plasmid
- / Photolysis
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