Abstract: A macrocyclic compound containing three spiropyran subunits(macrocyclic spiropyrans) has been synthesized.Click chemistry has been conducted in a dilute solution to construct the cyclic structure of the target product.The yield of macrocylic compound is 64%.The structure of the macrocyclic compounds is characterized by FT-IR,¹H NMR,¹³C NMR and ESI-MS.Cyclization of the macrocyclic spiropyrans is confirmed via MS analysis.The pH-responsive chromic properties of macrocyclic spiropyrans and linear compounds have been studied by using UV-Vis absorption spectroscopy in solutions at pH near 4.The maximum UV-Vis absorption(464 nm) of the macrocylic compound exhibits a blue shift comparing with that of linear compounds(476 nm).It is found that the molar extinction coefficient of the maximum UV-Vis absorption of macrocyclic spiropyrans is greater than those of its linear precursors.Furthermore,macrocyclic spiropyrans shows more sensitive pH-responsivity than its linear precursors.