引用本文:
郭蒙, 杨景和. 叶酸的能量转移荧光测定法及其应用[J]. 应用化学,
2011, 28(1): 101-106.
doi:
10.3724/SP.J.1095.2011.00174
Citation: GUO Meng, YANG Jinghe. A Fluorescent Energy Transfer Method and Its Application in the Detection of Folic Acid[J]. Chinese Journal of Applied Chemistry, 2011, 28(1): 101-106. doi: 10.3724/SP.J.1095.2011.00174
Citation: GUO Meng, YANG Jinghe. A Fluorescent Energy Transfer Method and Its Application in the Detection of Folic Acid[J]. Chinese Journal of Applied Chemistry, 2011, 28(1): 101-106. doi: 10.3724/SP.J.1095.2011.00174
叶酸的能量转移荧光测定法及其应用
English
A Fluorescent Energy Transfer Method and Its Application in the Detection of Folic Acid
Abstract:
The detection of folic acid by a fluorescent energy transfer method has been developed. In the folic acid-KMnO4-Eu3+-H2O2 system with the presence of HAc-NaAc buffer solution(pH=4.7,0.2 mol/L), the folic acid can be oxidized by KMnO4 with the presence of H2O2 and converted into the purine of butterfly-6-carboxyl acid, which can coordinate with Eu3+ forming a complex. The experimental conditions including the concentration of buffer solution and pH value, choice of oxidant, the temperature and heating time, the concentration of Eu3+, etc., were optimized. This system can emit the characteristic fluorescence of Eu3+ through an intra-molecule energy transfer process. The fluorescent intensity is in proportional to the concentration of folic acid in the range of 4×10-8~4×10-5 mol/L with correlation coefficient of 0.9902. The detection limit is found to be 1.2×10-8 mol/L. The developed method has been successfully applied to the determination of folic acid in the actual samples.
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Key words:
- folic acid
- / Eu
- / fluorescence
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